Identification of ace inhibitory cryptides in Tilapia protein hydrolysate by UPLC–MS/MS coupled to database analysis

Yesmine, Ben Henda; Antoine, Bonnet; Leocádia, Nunes Gonzalez da Silva Ortência; Rogério, Boscolo Wilson; Fruitier-Arnaudin, Ingrid; Bridiau, Nicolas; Maugard, Thierry; Piot, Jean–Marie; Frédéric, Sannier; Stéphanie, Bordenave-Juchereau

doi:10.1016/j.jchromb.2017.02.015

Cited by 22 publications

(11 citation statements)

References 34 publications

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“…VW was documented to be an ACEI dipeptide identied in many hydrolysates, including rapeseed, salmon protein, and tilapia proteins. [27][28][29] Furthermore, several other ACEI peptides were reported to share VW in their sequence, such as VWP (IC 50 ¼ 4.5 mM), VWY (IC 50 ¼ 9.4 mM), and DVWY (IC 50 ¼ 581.24 mM). Likewise, the ACEI peptides that shared the common FR fragment as part of their sequences were as follows: LFRQ (IC 50 ¼ 17 mM), AFKDEDTEEVPFR (IC 50 ¼ 80.2 mM), MILLLFR (IC 50 ¼ 0.12 mM), and LFR (IC 50 ¼ 31.8 mM).…”

Section: Identication and In Silico Analysis Of Peptidesmentioning

confidence: 99%

Two novel potent ACEI peptides isolated from Pinctada fucata meat hydrolysates using in silico analysis: identification, screening and inhibitory mechanisms

Chen

et al. 2021

RSC Adv.

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Section: Identication and In Silico Analysis Of Peptidesmentioning

confidence: 99%

Two novel potent ACEI peptides isolated from Pinctada fucata meat hydrolysates using in silico analysis: identification, screening and inhibitory mechanisms

Chen

et al. 2021

RSC Adv.

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“…Usually, methodologies are the same as those used to identify proteins in a shotgun proteomics approach: comparing fragmentation mass spectra within in silico species‐specific databank or towards de novo sequencing. To investigate the smallest peptides (di‐ or tri‐), however, time‐consuming sample preparation via membrane dialysis and chromatographic fractioning are necessary 10 …”

Section: Introductionmentioning

confidence: 99%

“…Liquid chromatography (LC) coupled to tandem mass spectrometry (MS/MS) has been one of the most commonly used apparatus for biopeptide identification in complex mixtures 10–13 . Therefore, our study aims to develop a fast LC–MS/MS method based on the neutral loss (NL) of 46 Da, in order to identify dipeptides and tripeptides candidates directly from protein hydrolysates, without the need of any laborious pre‐treatment or time‐consuming chromatographic separation.…”

Section: Introductionmentioning

confidence: 99%

Tri‐ and dipeptides identification in whey protein and porcine liver protein hydrolysates by fast LC–MS/MS neutral loss screening and de novo sequencing

et al. 2021

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We describe a fast (5 min) liquid chromatography tandem mass spectrometry method (LC–MS/MS) based on a 46 Da neutral loss of formic acid (H2O and CO) to identify tri‐ and dipeptides (DIPEP) in whey protein and porcine liver protein hydrolysates and confirmed by further de novo sequencing. Sample solutions were acidified to favor [dipep + H]+ ions, and a m/z range of 50–300 was used to improve sensitivity. All dipeptide candidates were selected based on all possibilities of the 20 amino acid combinations, and their collision‐induced dissociation fragments were screened via de novo sequencing. To determine their biological activities, sequenced dipeptides were compared with the Biopep database and other data from literature. Altogether, 18 dipeptides and 7 tripeptides were identified from the whey protein hydrolysate; they seemed to be broadly active, and peptides were identified as active dipeptidyl peptidase IV inhibitors and active angiotensin‐converting enzyme (ACE), according to available information. Porcine liver hydrolysate showed 14 dipeptides which exhibit similar biological activities to whey protein hydrolysate.

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“…Tilapia hydrolysate has many medicinal advantages, including high antioxidant activity in the skin [ 25 ]; the viscera suppress intracellular ROS in vitro [ 26 ]; the skin, bone, frame, head, muscle, and tail have high angiotensin-converting enzyme (ACE) inhibition activity [ 27 , 28 ]; and its viscera also reduce blood pressure, interleukin-6, and tumor necrosis factor-α expression [ 29 ]. Our preliminary study showed that tilapia hydrolysate contains adequate necessary amino acids and improved chemical characteristics, which could be met adult human nutritional needs.…”

Section: Introductionmentioning

confidence: 99%

Tilapia viscera hydrolysate extract alleviates oxidative stress and renal damage in deoxycorticosterone acetate-salt-induced hypertension rats

et al. 2020

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Background and Aim: Hypertension is closely related to oxidative stress conditions, which increases malondialdehyde (MDA) expression and renal damage. Tilapia viscera hydrolysate extract (TVHE) contains compounds and peptides that act as antioxidants. This study aimed to investigate TVHE therapy effect on MDA levels and renal histological conditions in deoxycorticosterone acetate (DOCA)-salt-induced hypertension rats. Materials and Methods: Tilapia viscera were defatted and hydrolyzed using Alcalase enzyme to obtain TVHE. TVHE antioxidant activity was measured using the 1,1-diphenyl-2-picrylhydrazyl method. Fifteen Wistar male rats were divided into five groups: Normal control (without induced DOCA-salt), DOCA-salt, DOCA-salt+Captopril 5 mg/kg body weight (BW), DOCA-salt+TVHE 150 mg/kg BW, and DOCA-salt+TVHE 300 mg/kg BW. MDA level and renal histology were observed in each group. Results: TVHE half maximal inhibitory concentration values ranged from 3.87±0.35 μg/mL to 42.03±3.55 μg/mL, which were identified as in the very strong Blois category. TVHE and captopril therapy reduced MDA expression significantly (p<0.05) compared to DOCA-salt only. TVHE and captopril therapy also improved glomerular damage in DOCA-salt-induced hypertension rats. Conclusion: TVHE has antioxidant ability, decreased MDA level, and decreased glomerular damage in DOCA-salt-induced hypertension rats.

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Identification of ace inhibitory cryptides in Tilapia protein hydrolysate by UPLC–MS/MS coupled to database analysis

Cited by 22 publications

References 34 publications

Two novel potent ACEI peptides isolated from Pinctada fucata meat hydrolysates using in silico analysis: identification, screening and inhibitory mechanisms

Two novel potent ACEI peptides isolated from Pinctada fucata meat hydrolysates using in silico analysis: identification, screening and inhibitory mechanisms

Tri‐ and dipeptides identification in whey protein and porcine liver protein hydrolysates by fast LC–MS/MS neutral loss screening and de novo sequencing

Tilapia viscera hydrolysate extract alleviates oxidative stress and renal damage in deoxycorticosterone acetate-salt-induced hypertension rats

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