Identification of Acid Fast Bacteria From Caseous Lesions in Cattle in Sudan

Abstract

One-hundred-and-twenty caseous lesions collected from slaughtered cattle at selected slaughterhouses in Sudan were processed for the detection of acid-fast bacteria (AFB). Sixty-four of the 120 samples showed AFB on microscopic examination after staining with the Ziehl-Neelsen method. Accordingly, it was estimated that 64 (53.3%) of the 120 caseous (purulent) lesions among the samples were due to AFB whereas 56 (46.7%) were due to other causes. Growth on Lowenstein-Jensen slants was obtained in 54 of the 120 samples. The isolated AFB were tentatively identified using microscopic and cultural characteristics. Confirmation of the phenotypic clusters was achieved by analysing the mycolic acids contents and PCR-amplification of the IS6110 insertion sequences. The above two methods have allowed the identification of Mycobacterium bovis and M. farcinogenes, the major AFB isolated from cattle in Sudan. The remaining AFB, which were negative for the above two tests, were further identified by sequencing the 16S rRNA gene. The above strategy thus allowed the identification of the isolated strains as follows: 25 (46%) M. bovis; 21 (39.9%) M. farcinogenes; 4 (7.4%) M. tuberculosis; 1 (1.9%) M. avium; 1 (1.9%) Nocardia sp., 2 (3.7%) unidentified AFB. The isolation of M. farcinogenes and M. tuberculosis, from pulmonary lymph nodes represented important findings.

Occurrence of bovine tuberculosis at Nyala abattoirs in South Darfur State, Sudan

“…Sulieman and Hamid (22) found that 64 (53.3%) of 120 caseous lesions from cattle in Eastern and Central Sudan were due to acid-fast bacilli (AFB), whereas 56 (46.7%) were due to other causes. Growth on Lowenstein-Jensen slants was obtained in 52 of the 120 samples and identified as follows: 25 (48.1%) Mycobacterium bovis, 21 (40.4%) M. farcinogenes, 4 (7.7%) M. tuberculosis, 1 (1.…”

Mycobacterial infections in carcasses of ruminants slaughtered at the two slaughterhouses of Kassala, Sudan

“…Animal reproduction and breeding as well as livestock trade at national and international levels are directly and adversely affected by mycobacterial infections (Radostits et al, 2007;Ehsan and Nganwa, 2014;Ramos et al, 2015). TB and other mycobacterial infections are quite prevalent in dairy growing mycobacterium species have also been detected (Sulieman and Hamid, 2002;Oloya et al, 2007;Osman, 2007;Manal et al, 2010;Asil et al, 2013;Aljameel et al, 2014;Nalapa et al, 2017). Environmental risk factors, malnutrition, presence of other diseases, and heavy infestation with endoparasites are important factors that increase the susceptibility of animals to TB (Radostits et al, 2007;Ehsan and Nganwa, 2014).…”

Diagnóstico histopatológico e molecular de lesões sugestivas de tuberculose em búfalos abatidos nos municípios de Macapá e Santana, estado do Amapá

“…A detecção de bacilos álcool-ácido resistentes (BAAR) em somente três búfalos demonstra a baixa sensibilidade da técnica, uma vez que 13 animais apresentaram resultados positivos nas técnicas de Nested-PCR. A baixa sensibilidade da coloração de Ziehl-Neelsen é relatada em bubalinos (Garine-Wichatitsky et al 2010, Laisse et al 2011) e em bovinos (Cancela & Marín 1993, Sulieman & Hamid 2002, Watrelot-Virieux et al 2006. Um animal que apresentava BAAR foi negativo aos testes moleculares.…”

“…(Muller et al 2011), Nocardia sp. (Sulieman & Hamid 2002), Streptococcus spp., ou serem decorrentes de micobacterioses atípicas, capazes de interferir em sistemas de PCR pouco sensíveis (Sahraoui et al 2009, Thacker et al 2011. Outra possibilidade seria a falha de sensibilidade das técnicas de Nested-PCR, uma vez que o diagnóstico diretamente em tecidos tem apresentado sensibilidade clínica média de até 75% (Araújo et al 2014a, Araújo et al 2014b.…”