Identification of Acinetobacter baumannii and Determination of MDR and XDR Strains

Ahmad, Noor Hussein; Mohammad, Ghada A.

doi:10.21123/bsj.2020.17.3.0726

Cited by 5 publications

(7 citation statements)

References 9 publications

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“…Once considered a low-grade pathogen, this aerobic Gram-negative coccobacillus is now a successful pathogen that causes opportunistic skin, bloodstream, urinary tract, and other soft tissue infections which is a dramatic increase in the number of A. baumannii infections among Iraq and Afghanistan war veterans and military. [32][33][34][35] As an example of an infection caused by A. baumannii, inflammation of the airways in the lungs, also known as pneumonitis. An infection with A. baumannii can spread from the mouth or nose to the lungs.…”

Section: Acinetobacter Baumanniimentioning

confidence: 99%

“…These are essential for blaOXA gene expression and carbapenem resistance. [34][35][36][37][38][39][40][41][42][43] Other MBL families, including VIM, IMP, GIM, SIM, and NDM enzymes, have been connected to the resistant phenotype in A. baumannii. [32][33][34][35][36][37][38][39][40] Finally, the significant prevalence of MDR A. baumannii in Saudi hospitals underscores the need of doing substantial research into the molecular basis of MDR and discovering novel therapeutics for CRAB.…”

Section: Acinetobacter Baumanniimentioning

confidence: 99%

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A Review: Hospital Acquired Infection in Some Gram Negative Pathogenic Bacterial Strains

Alkhowaiter,

AL-Jaddawi,

Abu-Zeid

et al. 2024

J. Contemp. Med. Sci.

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Objective: This review aims to update healthcare workers on the current scientific understanding of hospital-acquired infections, witha focus on describing the pathophysiology and patterns of antimicrobial resistance, particularly concerning Pseudomonas aeruginosa,Acinetobacter baumannii, and Klebsiella pneumoniae. Methods: Data on hospital-acquired infections were collected globally, with a specific emphasis on the Eastern Mediterranean, South-East Asia, Europe, and the Western Pacific regions. Infection rates, predominant pathogens, and antimicrobial resistance patterns were analyzed to provide insights into the current landscape of nosocomial infections. Results: Hospitals in the Eastern Mediterranean and South-East Asia regions reported the highest rates of nosocomial infections (11.8%and 10.0%, respectively), while rates in Europe and the Western Pacific were 7.7% and 9.0%, respectively. Infections typically arise frominvasive medical equipment and surgical operations, with lower respiratory tract and bloodstream infections being particularly hazardous. Gram-negative bacterial infections, notably, exhibit worrisome antibiotic resistance patterns, potentially developing multiple mechanisms against various antibiotics. Conclusion: The emergence of antimicrobial resistance presents a significant threat to patient safety, compounded by challenges indiscovering new antibiotics. Factors such as high costs and lengthy drug development processes contribute to this concern. Healthcareworkers must remain abreast of evolving antimicrobial resistance patterns, especially concerning pathogens like Pseudomonas aeruginosa, Acinetobacter baumannii, and Klebsiella pneumoniae, to implement effective infection control measures and preserve the efficacy of existing antimicrobial agents.

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Section: Acinetobacter Baumanniimentioning

confidence: 99%

Section: Acinetobacter Baumanniimentioning

confidence: 99%

A Review: Hospital Acquired Infection in Some Gram Negative Pathogenic Bacterial Strains

Alkhowaiter,

AL-Jaddawi,

Abu-Zeid

et al. 2024

J. Contemp. Med. Sci.

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“…Nineteen bacterial isolates of A.baumannii were taken from previous work [7] isolated from different clinical cases from Mosul hospitals/ Iraq, which included specimens from urine wounds, sputum, burns, CSF, and samples from the hospital environment including samples from intensive care unit, surgical instruments and appliances, patient beds, emergency lobby, and baby incubators. As well, standard strains of A. baumannii 19606 were used.…”

Section: Bacterial Isolatesmentioning

confidence: 99%

“…The results obtained in Table (3) were analyzed using the Jaccard coefficient mediated by the past3 program to find the similarity between bacterial isolates by obtaining the evolutionary tree diagram As shown in figure (7); the similarity between the isolates, six main clusters were obtained to combine. The first cluster (A) contained isolate No.…”

Section: Genetic Variation Of Abaumannii Isolatesmentioning

confidence: 99%

Detection of Some Virulence Genes and Genetic Variations of Acinetobacter Baumannii

Mohammad

Hussein

2021

Middle East Journal of Science

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Acinetobacter baumannii is gram-negative bacteria associated with hospital-acquired illnesses. The study involved molecular investigation of virulence genes of A. baumannii isolated from different sources using specific primers. Six genes were used for important virulence factors in this bacteria (ompA, plcN, csgA, lasB, iutA, fyuA), partially investigated using PCR, results showed the presence of these genes as 100% and 52.6% and 63.1% and 21% and 21% respectively, while the gene fyu A was not found in any A. baumanni isolate. The study also involved genetic variation in the DNA of these isolates using a universal primer (ERIC2). The results showed that 12 genotypes among 20 isolates of A.baumannii bacteria were revealed. The twelve isolates were divided into four groups, each of which contained genotype-identical isolates linked by bands with comparable molecular weights, as well as (8) single isolates that did not belong to any of the four groups.

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“…Acinetobacter baumannii has become a global health threat and burden, especially in hospitals. Acinetobacter species were found to be the reason behind a wide range of hospital-acquired infections such as nosocomial pneumonia, urinary tract infections, meningitis, septicemia, endocarditis, burn, and wound infections [1][2][3] . A. baumannii is the most outstanding and virulent reason for bacterial pathogens, the infections which are caused by A. baumannii complex (ACB) predominantly referred to A. baumannii infections 4 .…”

Section: Introductionmentioning

confidence: 99%

A Developed Colorimetric Assay Using Unmodified Gold Nanoparticles for the Identification of Acinetobacter baumannii Isolates from Different Clinical Samples

Sehree¹,

Al-Kaysi

Abdullah

2023

Baghdad Sci.J

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Acinetobacter baumannii (A. baumannii ) is considered a critical healthcare problem for patients in intensive care units due to its high ability to be multidrug-resistant to most commercially available antibiotics. The aim of this study is to develop a colorimetric assay to quantitatively detect the target DNA of A. baumannii based on unmodified gold nanoparticles (AuNPs) from different clinical samples (burns, surgical wounds, sputum, blood and urine). A total of thirty-six A. baumannii clinical isolates were collected from five Iraqi hospitals in Erbil and Mosul provinces within the period from September 2020 to January 2021. Bacterial isolation and biochemical identification of isolates were carried out followed by DNA extraction from 36 isolates and six negative ATCC strains (Salmonalle typhi, Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa, Enterobacter aeruginosa, Staphylococcus aures) and only one positive control ATCC A. baumannii using Phenol/Chloroform method. AuNPs were synthesized using the citrate reduction method and examined by XDR, FTIR, UV-VIS, FE-SEM, and TEM. The optimized colorimetric assay was employed based on unmodified spherical AuNPs and PCR amplification of 16S rRNA intergenic spacer sequences (ITS) with species-specific DNA oligo-targeters. Detection and optimization of A. baumannii amplicons using unmodified AuNPs were performed based on species-specific DNA oligonucleotide. The AuNPs assay was able to colorimetrically detect and distinguish A. baumannii from other ATCC bacterial isolates. The turnaround time of this assay was about 3 hours, including sample preparation and amplification, to show (0.025-6 ngµl-1) as a detection limit of DNA concentration. The efficacy of colorimetric detection was proved to effectively diagnose A. baumannii isolates with high sensitivity, simplicity, and robustness to rapidly diagnose A. baumannii isolates from different clinical samples.

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Identification of Acinetobacter baumannii and Determination of MDR and XDR Strains

Cited by 5 publications

References 9 publications

A Review: Hospital Acquired Infection in Some Gram Negative Pathogenic Bacterial Strains

A Review: Hospital Acquired Infection in Some Gram Negative Pathogenic Bacterial Strains

Detection of Some Virulence Genes and Genetic Variations of Acinetobacter Baumannii

A Developed Colorimetric Assay Using Unmodified Gold Nanoparticles for the Identification of Acinetobacter baumannii Isolates from Different Clinical Samples

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