Identification of Active Principles of M. balsamina (Balsam Apple) Leaf Extract

Karumi, Y; Onyeyili, P. A.; Ogugbuaja, V. O.

doi:10.3923/jms.2004.179.182

Cited by 68 publications

(9 citation statements)

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“…Identification of phytochemicals in the extract was performed following earlier reported methods. Briefly test for phenolics (ferric chloride test, phosphomolybdic acid test), flavonoids (NaOH test, Zn-HCl test, Shinoda test), saponins (foam test), tannins (Braemer’s test), steroids (Salkowski test), reducing (Fehling test) and non-reducing sugars (Iodine test) were performed [22].…”

Section: Methodsmentioning

confidence: 99%

1α, 25-dihydroxy Vitamin D3 containing fractions of Catharanthus roseus leaf aqueous extract inhibit preadipocyte differentiation and induce lipolysis in 3T3-L1 cells

Borah

Singh

Yasmin

et al. 2019

BMC Complement Altern Med

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BackgroundTo investigate the potential of Catharanthus roseus leaf aqueous crude extract (CRACE) as a regulator of adipocyte development and function.Methods3T3-L1 adipogenesis model was used to investigate the effect of CRACE on adipogenesis. 3T3-L1 preadipocytes (for adipogenic differentiation) and mature 3T3-L1 adipocytes (for adipocyte function) were treated with non-toxic doses of CRACE. The outcomes were corroborated by intracellular lipid accumulation, expression of pro-and anti-adipogenic effector molecules. To investigate CRACE mediated lipolysis, cAMP accumulation, glycerol release and phosphorylation of key effector molecules were tested in treated mature adipocytes. Finally, the extract was fractionated to identify the active molecule/s in the extract.ResultsCRACE significantly reduced adipocyte differentiation by modulating PPARγ expression. At early stage CRACE directly targeted Lipin1 expression and consequently impacted KLF7, subsequently expression of GATA2, CEBPα, SREBP1c were targeted, with PPARγ expression, particularly curtailed. While CRACE significantly reduced several lipogenic genes like FAS and GPD1 in mature adipocytes, concomitantly, it greatly increased lipolysis resulting in decreased lipid accumulation in mature adipocytes. The increase in lipolysis was due to decreased Akt activation, increased cAMP level, and PKA activity. The fractionation of CRACE allowed identification of two fractions with potent anti-adipogenic activity. Both the fractions contained 1α, 25-dihydroxy Vitamin D3 as major component.Conclusions1α, 25-dihydroxy Vitamin D3 containing CRACE can be developed into an effective anti-obesity formulation that decreases adipogenesis and increases lipid catabolism.

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Section: Methodsmentioning

confidence: 99%

1α, 25-dihydroxy Vitamin D3 containing fractions of Catharanthus roseus leaf aqueous extract inhibit preadipocyte differentiation and induce lipolysis in 3T3-L1 cells

Borah

Singh

Yasmin

et al. 2019

BMC Complement Altern Med

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“…Standard phytochemical analyses were carried out to test for the presence of the phytoconstituents in the extracts. Chemical tests were carried out on hydro-acetone, hydro-methanolic and aqueous extracts using standard methods for the detection of tannins (Stiasny test followed by ferric chloride test), flavonoids (Shibata's test) [10], steroids and triterpenoids (Liebermann-Buchard test) [11] alkaloïds (Valser-Mayer and Dragendorff's reagents tests), saponins (foaming index) [12].…”

Section: Qualitative Phytochemical Screeningmentioning

confidence: 99%

Antioxidant Activity of Hydro-Acetonic, Hydro-Methanolic and Aqueous Leaf and Bark Extracts of <i>Sclerocaria birrea</i> (A. Rich.) Hochst

Lahat¹,

Mahamat²,

Ayessou³

et al. 2021

FNS

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Bioactive compounds in plants are associated with the reduction of chronic diseases. The free radical scavenging activity of different extracts of a medicinal plant, Sclerocarya birrea, has been investigated using the DPPH test, ABTS test and FRAP. Three extracts were prepared from the leaves and bark: hydro-methanolic, hydro-acetonic and aqueous. Phytochemical screening was carried out the standard methods followed by the determination of the polyphenol by Folin-Ciocalteu method. The analysis of variance (ANOVA) with the STATISTICA 7.1 and statistical significance was set at p < 0.05. Evolution of percent inhibition (PI) as well as the IC 50 of the extracts was obtained using the Origin Pro 8.5 software and Microsoft Excel. The results show that the bark extracts are about twice as rich in polyphenols as the leaves. With DPPH at 1.25 mg/mL, the bark has a PI of 91.04% ± 0.001% while leaves, reach 99.80% ± 0.021%. As for the ABTS test, the bark extract reached its maximum activity at 1.25 mg/mL with a PI of 99.80% ± 0.003% while leaves extract greater value of PI is 99.75 ± 0.003 at 2.5 mg/mL. With FRAP test at 1.25 mg/mL, the bark has a PI of 79.29% ± 0.005% while leaves, reach 80.33% ± 0.001%. The IC 50 of the bark and leaf extracts on the smallest DPPH are 0.156 ± 0.001 mg/mL in hydro-methanol, 0.301 ± 0.00 mg/mL in hydro-acetone and 0.407 ± 0.00 mg/mL in aqueous extract. With ABTS test, the best IC 50 are obtained with hydro-acetone extracts with value of 0.247 ± 0.001 mg/mL for bark and 0.248 ± 0.0005 mg/mL for leaves while in hydro-methanolic and aqueous extracts the best IC 50 are respectively 0.255 ± 0.00 mg/mL and 0.463 ± 0.00 mg/mL. Using ascorbic acid as our standard, the PI was 94.86% ± 0.008% with an IC 50 of 0.213 ± 0.00 mg/mL. According to these results, the reducing power of the bark is slightly higher than that of

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“…Standard phytochemical analysis methods were used to test for the presence of phytoconstituents in the extracts. Chemical tests were carried out on hydro-acetone, hydro-methanolic and aqueous extracts using standard methods for the detection of tannins (Stiasny test followed by ferric chloride test), flavonoids (Shibata's test) [15], steroids and triterpenoids (Liebermann-Buchard test) [16] alkaloïds (Valser-Mayer and Dragendorff's reagents tests), saponins (foaming index) [17].…”

Section: Qualitative Phytochemical Screeningmentioning

confidence: 99%

Phytochemical Screening, Total Phenolic and Antioxidant Activity of Numerous Extracts from Leaves and Bark of <i>Bauhinia rufescens</i> Lam

Mahamat¹,

Lahat²,

Cyrille³

et al. 2021

FNS

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Researches for natural antioxidants that play an important role in the prevention of diseases linked to free radicals have increased in recent years. In this study, antioxidant activity of Bauhinia rufescens Lam which is a medicinal plant was investigated. The methanol extract, acetone extract and water extract of leaf and trunk bark had antioxidant activity. Three methods were used: 2,2-diphenyl-1picryl-hydrazyl (DPPH), 2,2'-Azino-bis-(3-ethylbenzoth iazoline-6-sulfonic acid) (ABTS) and ferric ion reducing power (FRAP) by spectrophotometry. Phytochemical screening was carried out followed by the quantification of the total phenolic by the Folin-Ciocalteu method. The analysis of variance (ANOVA) with the STATISTICA 7.1 software revealed significant differences (p < 0.05). Percentage inhibition (PI) and IC 50 of the extracts were obtained using the OriginPro 8.5 software. These tests show that the bark is as rich in total phenolic as the leaves. The DPPH test shows that at 2.5 mg/ml, the bark and the leave have similar activity. Bark has a percentage inhibition (PI) of 86.55% ± 0.026% compared to the leave which PI is 85.63% ± 0.02%. As for the ABTS test, the bark extract reached its maximum activity at 2.5 mg/ml with a PI of 99.81% ± 0.012% compared to the leaf extract which has a PI of 99.61% ± 0.025%. The best IC 50 of the extracts obtained with the DPPH radical is 0.139 ± 0.001 mg/ml (hydro-acetonic), 0.354 ± 0.001 mg/ml (hydromethanolic) and 0.840 ± 0.001 mg/ml (aqueous) on the bark. With ABTS test, it was obtained 0.351 ± 0.001 mg/ml (hydro-acetone; bark), 0.403 ± 0.001 mg/ml (hydro-acetone; leaves) and 0.474 ± 0.001 mg/ml (aqueous; bark). The reducing power of the leaves is slightly higher than that of the bark. Standard of ascorbic acid, has a PI of 94.86 ± 0.008% with an IC 50 of 0.213 ± 0.001 mg/ml. Thus, it is easy to conclude that the bark of Bauhinia rufescens has a better activity than the leaves and the alcoholic extracts have given better re-

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Identification of Active Principles of M. balsamina (Balsam Apple) Leaf Extract

Cited by 68 publications

References 1 publication

1α, 25-dihydroxy Vitamin D3 containing fractions of Catharanthus roseus leaf aqueous extract inhibit preadipocyte differentiation and induce lipolysis in 3T3-L1 cells

1α, 25-dihydroxy Vitamin D3 containing fractions of Catharanthus roseus leaf aqueous extract inhibit preadipocyte differentiation and induce lipolysis in 3T3-L1 cells

Antioxidant Activity of Hydro-Acetonic, Hydro-Methanolic and Aqueous Leaf and Bark Extracts of <i>Sclerocaria birrea</i> (A. Rich.) Hochst

Phytochemical Screening, Total Phenolic and Antioxidant Activity of Numerous Extracts from Leaves and Bark of <i>Bauhinia rufescens</i> Lam

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Identification of Active Principles of M. balsamina (Balsam Apple) Leaf Extract

Cited by 68 publications

References 1 publication

1α, 25-dihydroxy Vitamin D3 containing fractions of Catharanthus roseus leaf aqueous extract inhibit preadipocyte differentiation and induce lipolysis in 3T3-L1 cells

1α, 25-dihydroxy Vitamin D3 containing fractions of Catharanthus roseus leaf aqueous extract inhibit preadipocyte differentiation and induce lipolysis in 3T3-L1 cells

Antioxidant Activity of Hydro-Acetonic, Hydro-Methanolic and Aqueous Leaf and Bark Extracts of &lt;i&gt;Sclerocaria birrea&lt;/i&gt; (A. Rich.) Hochst

Phytochemical Screening, Total Phenolic and Antioxidant Activity of Numerous Extracts from Leaves and Bark of &lt;i&gt;Bauhinia rufescens&lt;/i&gt; Lam

Contact Info

Product

Resources

About

Antioxidant Activity of Hydro-Acetonic, Hydro-Methanolic and Aqueous Leaf and Bark Extracts of <i>Sclerocaria birrea</i> (A. Rich.) Hochst

Phytochemical Screening, Total Phenolic and Antioxidant Activity of Numerous Extracts from Leaves and Bark of <i>Bauhinia rufescens</i> Lam