Identification of Alkaloid’s Profile in Ficus benjamina L. Extracts with Higher Antioxidant Power

Abstract

Different classes of known alkaloid compounds were identified for the first time in leaves and barks of Ficus benjamina L. from Moraceae family. The identification of 28 alkaloids in F. benjamina leaves and 14 alkaloids in its barks was identified using chromatographic analysis as Gas Chromatography associated to Mass Spectrometry (GC-MS). This characterization analysis allows to verifying the presence of different alkaloids type-classes in both leaves and barks, such as isoquinoline class, indole class and quinolizidine class. The quantification results are deduced by analysis with spectrophotometric assay, using a calibration curve of boldine as standard alkaloid that reveals the presence of 26 ± 0.1 μg of alkaloids boldine equivalent in 1 gram of F. benjamina leaves and 3.8 ± 0.1 μg alkaloids boldine equivalent in 1 gram of its barks. A significant correlation was observed between the total alkaloid content of crude extracts and 2,2-diphenyl-1-picrylhydrazyl assay (DPPH), suggesting that the level of antioxidant activity in this species is strongly correlated to the alkaloid content. These findings should provide useful information for future on the pharmaceutical properties of these alkaloids from F. benjamina.

Phytochemical Screening and Antioxidant Activity of Selekop (Lepisanthes amoena) Fruit

“…Alkaloids are one of the secondary metabolites found in plants, which can be found in leaves, branches, seed, and bark, (Aksara, Musa, & Alio, 2013). Novelli, Lorena, & Antonella (2014) reported that alkaloid contained in Ficus benjamina L. crude extract, had a strong correlation with its antioxidant.…”

Heat stress increases the use of cytosolic pyruvate for isoprene biosynthesis

“…For example, P. aquatica accumulates high contents of the fungitoxin isohemigossypolone in the outer bark of the below-ground part of the swollen trunk (Shibatani et al , 1999) and it has been found to be a weak isoprene emitter (Taylor et al , 2018). Ficus species contain numerous secondary plant metabolites such as alkaloids (Baumgartner et al , 1990; Khan et al , 1993; Novelli et al , 2014) and in contrast to P. aquatica they have been found to be strong isoprene emitters (Wyche et al , 2014). Many bioactive constituents such as cinnamic acids, lactose, naringenin, quercetin, caffeic acid, and stigmasterol as well as 28 different alkaloids have been identified in F. benjamina (Novelli et al , 2014).…”

The Plant Natural Products: Their Antioxidants, Free Radical Scavengers, DNA Protection and Antimicrobial Activities

“…In addition, the purified alkaloids from Amaranthus viridis leaves have protective effects in vitro conditons against hydrogen peroxide induced oxidative damage in human erythrocytes through decreasing Lipid peroxidation levels [55]. On the same side, Novelli et al [56] identified of 28 alkaloids in leaves of Ficus benjamina and 14 alkaloids in its barks. A significant correlation was observed between the total alkaloid content of crude extracts and 2,2-diphenyl-1-picrylhydrazyl assay (DPPH), suggesting that the level of antioxidant activity in this species is strongly correlated to the alkaloid content.…”