Identification of alternative oxidase encoding genes in Caulerpa cylindracea by de novo RNA-Seq assembly analysis

Ünlü, Ercan Selçuk; Ünüvar, Ömer Can; Aydın, Meryem

doi:10.1016/j.margen.2019.03.004

Cited by 6 publications

(5 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has also been confirmed that AOX plays an important role in maintaining the normal operation of photosynthesis when plants are under abiotic stresses [ 22 , 26 , 44 ]. In macroalgae, AOX plays an important role during the stress resistance process, and AOX is essential in maintaining the redox homeostasis of algal chloroplast development during greening [ 30 , 37 ]. Compared with the control group (NL), the AOX activity of thalli increased significantly under PAR + UVB radiation, while there was no significant change in the thalli under HL radiation.…”

Section: Discussionmentioning

confidence: 99%

“…There is relatively little research on the role of AOX during the environmental response of macroalgae. RNA-seq analysis demonstrated that AOX played an important role in stress resistance and invasion processes in the macroalgae Caulerpa cylindracea [ 37 ]. In Auxenochlorella protothecoides , AOX was essential in promoting ROS scavenging and maintaining redox homeostasis for algal chloroplast development during greening [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

“…Alternative oxidase (AOX) exists in plants in addition to the cytochrome oxidase (COX) respiratory pathway. The electrons that accept reductive ubiquinone directly reduce oxygen to water [22][23][24][25], so the electron flow of the AOX respiratory pathway bypasses the last two energy conservation sites (complexes III and IV) related to the COX respiratory pathway [26]. Because of this property, which has nothing to do with energy conservation, this can alleviate an imbalance in carbon and energy metabolism caused by stress [27].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Acclimation of intertidal macroalgae Ulva prolifera to UVB radiation: the important role of alternative oxidase

Xu,

Zhao,

Zhong

et al. 2024

BMC Plant Biol

View full text Add to dashboard Cite

Background Solar radiation is primarily composed of ultraviolet radiation (UVR, 200 − 400 nm) and photosynthetically active radiation (PAR, 400 − 700 nm). Ultraviolet-B (UVB) radiation accounts for only a small proportion of sunlight, and it is the primary cause of plant photodamage. The use of chlorofluorocarbons (CFCs) as refrigerants caused serious ozone depletion in the 1980s, and this had led to an increase in UVB. Although CFC emissions have significantly decreased in recent years, UVB radiation still remains at a high intensity. UVB radiation increase is an important factor that influences plant physiological processes. Ulva prolifera, a type of macroalga found in the intertidal zone, is intermittently exposed to UVB. Alternative oxidase (AOX) plays an important role in plants under stresses. This research examines the changes in AOX activity and the relationships among AOX, photosynthesis, and reactive oxygen species (ROS) homeostasis in U. prolifera under changes in UVB and photosynthetically active radiation (PAR). Results UVB was the main component of solar radiation impacting the typical intertidal green macroalgae U. prolifera. AOX was found to be important during the process of photosynthesis optimization of U. prolifera due to a synergistic effect with non-photochemical quenching (NPQ) under UVB radiation. AOX and glycolate oxidase (GO) worked together to achieve NADPH homeostasis to achieve photosynthesis optimization under changes in PAR + UVB. The synergism of AOX with superoxide dismutase (SOD) and catalase (CAT) was important during the process of ROS homeostasis under PAR + UVB. Conclusions AOX plays an important role in the process of photosynthesis optimization and ROS homeostasis in U. prolifera under UVB radiation. This study provides further insights into the response of intertidal macroalgae to solar light changes.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Acclimation of intertidal macroalgae Ulva prolifera to UVB radiation: the important role of alternative oxidase

Xu,

Zhao,

Zhong

et al. 2024

BMC Plant Biol

View full text Add to dashboard Cite

show abstract

“…In 2007 and 2009, the genes encoding AOX were isolated from methylotrophic yeast Pichia pastoris , the thermogenic tissue of Symplocarpus renifolius , and nonthermogenic Lysichiton camtschatcensis . In recent decades, numerous new technologies have emerged permitting researchers to acquire the AOX proteins, such as cloning and functional expression of the AOX1 of Aspergillus niger in Lactococcus lactis , expression of recombinant S. guttatum AOX in Escherichia coli membranes, heterologous expression of the AOX from Crassostrea gigas (Pacific oyster) and Moniliophthora perniciosa in the yeast S. cerevisiae , , self-assembled proteoliposomes to functionally characterize the AOX from M. perniciosa , and identification of AOX encoding genes in Caulerpa cylindracea by de novo RNA sequencing (RNA-Seq) assembly analysis . The heterologous expression of AOX has made significant contributions to the research of function and mechanism of AOX.…”

Section: Discovery Of Aoxmentioning

confidence: 99%

Alternative Oxidase: From Molecule and Function to Future Inhibitors

Li,

Yang,

et al. 2024

ACS Omega

View full text Add to dashboard Cite

In the respiratory chain of the majority of aerobic organisms, the enzyme alternative oxidase (AOX) functions as the terminal oxidase and has important roles in maintaining metabolic and signaling homeostasis in mitochondria. AOX endows the respiratory system with flexibility in the coupling among the carbon metabolism pathway, electron transport chain (ETC) activity, and ATP turnover. AOX allows electrons to bypass the main cytochrome pathway to restrict the generation of reactive oxygen species (ROS). The inhibition of AOX leads to oxidative damage and contributes to the loss of adaptability and viability in some pathogenic organisms. Although AOXs have recently been identified in several organisms, crystal structures and major functions still need to be explored. Recent work on the trypanosome alternative oxidase has provided a crystal structure of an AOX protein, which contributes to the structure−activity relationship of the inhibitors of AOX. Here, we review the current knowledge on the development, structure, and properties of AOXs, as well as their roles and mechanisms in plants, animals, algae, protists, fungi, and bacteria, with a special emphasis on the development of AOX inhibitors, which will improve the understanding of respiratory regulation in many organisms and provide references for subsequent studies of AOX-targeted inhibitors.

show abstract

“…The enzyme is characterized by cyanide resistance and is associated with maintenance of metabolic homeostasis via mitochondrial electron transport in plants subjected to various stress conditions 1 , 2 . In plants, AOX is considered a ubiquitous enzyme; however, in algae 3 , fungi 4 – 9 , protozoa 10 – 13 and several invertebrate species 14 it is quite sporadic. AOX is involved in the mitochondrial respiratory chain and provides an alternative pathway for the direct transfer of electrons from ubiquinone to oxygen, thus forming water 15 .…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and gene expression modulation of the alternative oxidase in a scuticociliate parasite by hypoxia and mitochondrial respiration inhibitors

Folgueira

Lamas

Sueiro

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Philasterides dicentrarchi is a marine benthic microaerophilic scuticociliate and an opportunistic endoparasite that can infect and cause high mortalities in cultured turbot (Scophthalmus maximus). in addition to a cytochrome pathway (cp), the ciliate can use a cyanide-insensitive respiratory pathway, which indicates the existence of an alternative oxidase (AoX) in the mitochondrion. Although AoX activity has been described in P. dicentrarchi, based on functional assay results, genetic evidence of the presence of AoX in the ciliate has not previously been reported. in this study, we conducted genomic and transcriptomic analysis of the ciliate and identified the AOX gene and its corresponding mRNA. The AOX gene (size 1,106 bp) contains four exons and three introns that generate an open reading frame of 915 bp and a protein with a predicted molecular weight of 35.6 kDa. The amino acid (aa) sequence of the AoX includes an import signal peptide targeting the mitochondria and the protein is associated with the inner membrane of the mitochondria. Bioinformatic analysis predicted that the peptide is a homodimeric glycoprotein, although monomeric forms may also appear under native conditions, with eXXH motifs associated with the diiron active centers. the aa sequences of the AOX of different P. dicentrarchi isolates are highly conserved and phylogenetically closely related to AOXs of other ciliate species, especially scuticociliates. AOX expression increased significantly during infection in the host and after the addition of CP inhibitors. This confirms the important physiological roles of AoX in respiration under conditions of low levels of o 2 and in protecting against oxidative stress generated during infection in the host. Alternative oxidase (AOX), also known as ubiquinol-oxidoreductase, is included within the diiron carboxylate superfamily. The enzyme is characterized by cyanide resistance and is associated with maintenance of metabolic homeostasis via mitochondrial electron transport in plants subjected to various stress conditions 1,2. In plants, AOX is considered a ubiquitous enzyme; however, in algae 3 , fungi 4-9 , protozoa 10-13 and several invertebrate species 14 it is quite sporadic. AOX is involved in the mitochondrial respiratory chain and provides an alternative pathway for the direct transfer of electrons from ubiquinone to oxygen, thus forming water 15. It facilitates mitochondrial respiratory activity and therefore the functioning of the Krebs cycle after inhibition of the cytochrome route due to the presence of nitric oxide, cyanide (both inhibitors of the complex IV) or antimycin A (complex III inhibitor) 1,12,16. However, AOX reduces the energy efficiency of respiration as only 1/3 of the ATP is produced

show abstract

Identification of alternative oxidase encoding genes in Caulerpa cylindracea by de novo RNA-Seq assembly analysis

Cited by 6 publications

References 41 publications

Acclimation of intertidal macroalgae Ulva prolifera to UVB radiation: the important role of alternative oxidase

Acclimation of intertidal macroalgae Ulva prolifera to UVB radiation: the important role of alternative oxidase

Alternative Oxidase: From Molecule and Function to Future Inhibitors

Molecular characterization and gene expression modulation of the alternative oxidase in a scuticociliate parasite by hypoxia and mitochondrial respiration inhibitors

Contact Info

Product

Resources

About