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The aim of this study is to investigate genetic diversity in 48 einkorn (Triticum monococcum L. ssp. monococcum) landraces grown in agricultural areas of Bolu and Kastamonu. Therefore, variation in seven agro-morphological traits was investigated. Agro-morphological traits such as leaf weight (mg), coleoptile length (cm), root number (n), root length (cm), fresh root weight (mg), and dry root weight (mg) were examined by the coefficient of variation, ANOVA, and principal components analysis. The highest coefficient of variation (%) was observed in fresh root weight (FRW = 52.09%), while the lowest was in leaf weight (LW = 8.9%). Principal Component Analysis (PCA) was calculated as 76.93% variation in two main components. For molecular characterization, data obtained with iSSR primers were analyzed with the population genetics analysis program PopGene (ver. 1.32). According to PopGene results, the mean number of alleles, the mean number of effective alleles, and average genetic diversity values were calculated as na = 2, nea = 1.33, and h = 0.13, respectively. Among the agro-morphological traits, germination power, root number, and coleoptile length appeared to be reliable traits. The results show that the use of morphological characters alone for genetic diversity in populations is not sufficient to determine the difference between populations and their genetic structure.

Tetradesmus obliquus is a unicellular green microalga and considered as a potential source for biotechnological production of pigments such as lutein. No genome-related data is available for T. obliquus that would increase the ability to develop new approaches in biotechnological applications. We present the first transcriptome data for T. obliquus. The aim of this study is to provide a comprehensive transcriptome annotation and identification of conserved genes involved in lutein pigment biosynthesis in Tetradesmus obliquus cells by analyzing pooled RNA-Seq data. Next-Generation Sequencing was applied for the pooled cDNAs library prepared by combining the cell cultures collected from samples exposed to dark and high light intensity conditions. Transcripts were assembled by the de novo assembly approach. Trinotate software was used for functional annotation of assembled transcripts. We also carried out BLAST analysis comparing the transcriptome data against known lutein biosynthesis genes. The 49.15% of the assembled sequences were functionally annotated, providing a total of 21490 unigenes. Our data also revealed the transcript sequences for ten conserved genes required for lutein biosynthesis. The data produced in this study can be used for molecular approaches in biotechnological applications related to T. obliquus, such as increasing the yield of pigment production.

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