Identification of an adaptor protein‐2 mu gene (AccAP2m) in Apis cerana cerana and its role in oxidative stress responses

Abstract

The adaptor protein‐2 (AP‐2) complex refers to a class of proteins that play an important role in cargo recognition and are involved in clathrin‐mediated endocytosis of cargo proteins from the plasma membrane in animal cells. However, there have been few studies of AP‐2 in insects. Here, we isolated an AP‐2 gene from Apis cerana cerana (A. cerana cerana) and explored the function of this gene, named AccAP2m. The obtained amino acid sequence of AccAP2m is highly conserved across species and contains conserved features of the AP‐2 family. Quantitative real‐time PCR analysis showed that AccAP2m mRNA levels were high in newly emerged workers, and the expression levels were higher in muscle than in other tissues. The expression of AccAP2m was altered in response to various environmental stress factors. Moreover, overexpression of recombinant AccAP2m protein enhanced the resistance of the bacteria to HgCl2, CdCl2, and cumyl hydroperoxide. In addition, after knockdown of AccAP2m in A. cerana cerana by RNA interference (RNAi), the transcription of some antioxidant genes was downregulated, and the activities of some antioxidant enzymes decreased. Taken together, these results indicate that AccAP2m may play an indispensable role in response to oxidative stresses.

Role of c‐Jun NH2‐terminal kinase‐mediated mitogen‐activated protein kinase pathway in response to pesticides in Apis cerana cerana

“…On the basis of the cDNA sequences, primers (as shown in Table 1) were designed and synthesized by Sangon Biotech Company (Shanghai, China). The ORFs of AccJNK (GenBank ID: XM_017055351.2) and AccAP‐1 (GenBank ID: MF994311.1) were cloned by PCR using the following procedure: 10 min at 94 °C, 34 cycles of 40 s at 94 °C, 40 s at 56 °C, and 2 min at 72 °C and 10 min at 72 °C (Wang et al ., 2019b). The PCR products were purified using a Gel Extraction Kit (Solarbio, Beijing, China) and ligated individually into the cloning vector pMD19‐T (TransGen Biotech).…”

Molecular genetic study on GATA5 gene promoter in acute myocardial infarction

“…Theses TFs were involved in a variety of biological processes such as DNA repair, DNA replication, differentiation, proliferation, apoptosis, anti-inflammatory activity and lipid metabolism [23][24][25][26][27][28][29][30][31][32].…”