Identification of an Arabidopsis Nodulin-Related Protein in Heat Stress

Fu, Qi; Li, Shujia; Yu, Diqiu

doi:10.1007/s10059-010-0005-3

Cited by 17 publications

(8 citation statements)

References 51 publications

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“…At2g03440 encodes the nodulin-related protein AtNRP1. Overexpression of AtNRP1 results in reduced synthesis of ABA and lower resistance to heat stress in seedlings (Fu et al, 2010). This result suggests that papillae express factors involved in ABA synthesis, regulating the response to environmental stresses, such as drought, heat and salinity (Cutler et al, 2010;Golldack et al, 2014).…”

Section: Resultsmentioning

confidence: 97%

Abscisic acid-mediated developmental flexibility of stigmatic papillae in response to ambient humidity in <i>Arabidopsis thaliana</i>

et al. 2018

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Stigmatic papillae develop at the apex of the gynoecium and play an important role as a site of pollination. The papillae in Brassicaceae are of the dry and unicellular type, and more than 15,000 genes are expressed in the papillae; however, the molecular and physiological mechanisms of their development remain unknown. We found that the papillae in Arabidopsis thaliana change their length in response to altered ambient humidity: papillae of flowers incubated under high humidity elongated more than those under normal humidity conditions. Genetic analysis and transcriptome data suggest that an abscisic acid-mediated abiotic stress response mechanism regulates papilla length. Our data suggest a flexible regulation of papilla elongation at the post-anthesis stage, in response to abiotic stress, as an adaptation to environmental conditions.

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Section: Resultsmentioning

confidence: 97%

Abscisic acid-mediated developmental flexibility of stigmatic papillae in response to ambient humidity in <i>Arabidopsis thaliana</i>

et al. 2018

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“…In addition, a nodulin-related protein 1 (NPR1, spot 45) was more abundant in PsCA+CS fruit peel than in the control. As NRP1 was required for defense responses against cold stress and avirulent bacteria (Quirino et al, 2004 ; Fu et al, 2010 ), this suggests that NRP1 may play a role in PsCA-induced defense against chilling and bacterial diseases.…”

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of Differentially Accumulated Proteins in Cucumber (Cucumis sativus) Fruit Peel in Response to Pre-storage Cold Acclimation

2018

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Harvested fruits are still living organs and respond to environmental stimuli. Low temperature storage is effective in extending life of harvested fruit, but it may also cause chilling injury. Cold acclimation has been shown to induce chilling tolerance in plants, but what proteomic changes caused by cold acclimation are related to defense against chilling stress remains largely unclear. Here, 3 d of pre-storage cold acclimation (PsCA) at 10°C reduced chilling injury and secondary disease severity in cucumber stored at 5°C by 51 and 94%, respectively, compared with the control which was directly stored at 5°C. Proteomic analysis of cucumber peel identified 21 significant differentially-accumulated proteins (SDAPs) right after PsCA treatment and 23 after the following cold storage (PsCA+CS). These proteins are mainly related to stress response and defense (SRD), energy metabolism, protein metabolism, signal transduction, primary metabolism, and transcription. The SRD proteins, which made up 37% of the 21 and 47% of the 23, respectively, represented the largest class of SDAPs, and all but one protein were up-regulated, suggesting accumulation of proteins involved in defense response is central feature of proteomic profile changes brought about by PsCA. In fruit just after PsCA treatment, the identified SDAPs are related to responses to various stresses, including chilling, salt stress, dehydration, fungi, bacteria, insects, and DNA damage. However, after prolonged cold storage, the targeted proteins in acclimated fruit were narrowed down in scope to those involved in defense against chilling and pathogens. The change patterns at the transcription level of the majority of the up-regulated differentially-accumulated proteins were highly consistent with those at protein level. Taken all, the results suggest that the short-time cold acclimation initiated comprehensive defense responses in cucumber fruit at first, while the long term storage thereafter altered the responses more specifically to chilling. These findings add to the understanding of plants' molecular responses to cold acclimation.

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“…NHX1, involved in preventing leakage of electrolytes and nutrients through the plant cell membrane, which appears during the bacterial pathogen infection in plants (Brisset et al 1990), is putatively targeted by four novel miRNAs: mdm-miR142N, mdmmiR143N, mdm-miR144N, and mdm-miR145N. Nodulin, which is known to be expressed in response to bacterial infection (Fu et al 2010;Becker et al 2001), is potentially targeted by apple-specific mdm-miR535b,c,d. The third gene, a transcript highly similar to dinelactone hydrolase protein family, was predicted to be targeted by conserved miR159c.…”

Section: Discussionmentioning

confidence: 99%

“…Nodulins are expressed in response to bacterial infection and are involved in nodule formation in legumes. Nodulin-related proteins were also reported to play a role in pathogen and heat stress tolerance in A. thaliana (Fu et al 2010) as well as they may be markers of Rhisobium infection in Vicia faba (Becker et al 2001). The third gene, a transcript highly similar to dinelactone hydrolase protein family, was predicted to be targeted by conserved miR159c.…”

Section: Target Predictionmentioning

confidence: 99%

Identification of apple miRNAs and their potential role in fire blight resistance

Kaja

Szcześniak

Jensen

et al. 2014

Tree Genetics & Genomes

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MicroRNAs (miRNAs) are key players in multiple biological processes; therefore, analysis and characterization of these small regulatory RNAs is a critical step toward a better understanding of animal and plant biology. In apple (Malus domestica), 200 microRNAs are known, which most probably represent only a fraction of miRNAome diversity. As a result, more effort is required to better annotate miRNAs and their functions in this economically important species. We performed deep sequencing of 12 small RNA libraries obtained for fire blight-resistant and fire blight-sensitive trees. In the sequencing results, we identified 116 novel microRNAs and confirmed a majority of previously reported apple miRNAs. We then experimentally verified selected candidates with RT-PCR and stem-loop quantitative PCR (qPCR) and performed differential expression analysis. Finally, we identified and characterized putative targets of all known apple miRNAs. The gene ontology (GO) enrichment analysis suggests prominent roles of miRNAs in response to stresses, including pathogen infection. In this study, we identified 116 new and confirmed the expression of 143 already known miRNAs. Moreover, our data suggests that apple microRNAs might be considered as regulators and markers of fire blight resistance. The analyses we performed allowed us to define four apple miRNAs potentially involved in fire blight resistance in apple trees: mdm-miR169a, mdm-miR160e, mdm-miR167b-g, and mdm-miR168a,b. These miRNAs are known to be involved in response to stresses across other plant species, usually by targeting stress response proteins. The relatively low number of candidates may result from the high variance of biological replicates and the fact that stress response miRNAs are usually induced by the stress factors and frequently expressed at a low level, or not expressed at all, in normal conditions. The results of our studies are freely available in an online database at

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Identification of an Arabidopsis Nodulin-Related Protein in Heat Stress

Cited by 17 publications

References 51 publications

Abscisic acid-mediated developmental flexibility of stigmatic papillae in response to ambient humidity in <i>Arabidopsis thaliana</i>

Abscisic acid-mediated developmental flexibility of stigmatic papillae in response to ambient humidity in <i>Arabidopsis thaliana</i>

Proteomic Analysis of Differentially Accumulated Proteins in Cucumber (Cucumis sativus) Fruit Peel in Response to Pre-storage Cold Acclimation

Identification of apple miRNAs and their potential role in fire blight resistance

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