Identification of an epithelial-mesenchymal transition-related lncRNA prognostic signature for patients with glioblastoma

Xiao-jun, Yang; Niu, Sha; Liu, JiaQiang; Fang, Jincheng; Wu, Zeyu; Ling, Shizhang; Di, Guangfu; Jiang, Xiaochun

doi:10.1038/s41598-021-03213-y

Cited by 12 publications

(9 citation statements)

References 45 publications

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“…Accumulating studies have uncovered that circRNAs contributing to stronger proliferation ability and weaker apoptosis ability of cancer cells are also validated to facilitate GBM tumorigenesis and progression in vivo [47,48]. Moreover, the aberrant expression of genes has been suggested to relate to the prognosis of GBM patients [49,50]. In this study, animal experiments and clinical samples were lacked, thus the role of circGLIS3 in GBM progression and the association between circGLIS3 expression and the prognosis of GBM patients with could not be investigated.…”

Section: Discussionmentioning

confidence: 97%

A circular RNA derived from GLIS3 accelerates the proliferation of glioblastoma cells through competitively binding with miR-449c-5p to upregulate CAPG and GLIS3

et al. 2022

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Background Glioblastoma (GBM) is an aggressive and malignant brain tumor with extremely poor prognosis. Despite advances in treatment, the pathogenesis of GBM remains elusive. Mounting studies have revealed the critical role of circular RNAs (circRNAs) in the development and progression of human cancers including GBM, but the comprehension of their functions is still insufficient. In this study, we investigated the expression profile of a circRNA derived from GLIS family zinc finger 3 (GLIS3) in GBM and normal astrocytes. CircGLIS3 expression was detected through quantitative real-time polymerase chain reaction (qRT-PCR) analysis. Functional experiments were performed to analyze the influence of circGLIS3 on GBM cell proliferation and apoptosis. In addition, mechanism assays were to uncover the potential regulatory mechanism of circGLIS3. Results CircGLIS3 was up-regulated in GBM cells and knockdown of circGLIS3 significantly hampered proliferation and promoted apoptosis of GBM cells. Furthermore, circGLIS3 positively regulated CAPG and GLIS3 by sponging miR-449c-5p to affect GBM cell proliferation and apoptosis. Conclusions In summary, our study identified that circGLIS3 could promote proliferation and inhibit apoptosis of GBM cells via targeting miR-449c-5p/GLIS3/CAPG axis in vitro. This study could offer a novel molecular perspective for further investigation into mechanisms essential to GBM progression.

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Section: Discussionmentioning

confidence: 97%

A circular RNA derived from GLIS3 accelerates the proliferation of glioblastoma cells through competitively binding with miR-449c-5p to upregulate CAPG and GLIS3

et al. 2022

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“…Additionally, several research reported GSEC could promote the malignant process of triple-negative breast cancer and hepatocellular carcinoma via the GSEC/miR-202-5p/AXL axis and the GSEC/miR-101-3p/SNX16/PAPOLG axis [38,39], respectively. Furthermore, lncRNA AL606834.1 has been used for prognostic models of lung adenocarcinoma and pancreatic cancer [40,41], AL365181.2 has been applied in multiple prognostic models of lung adenocarcinoma [42,43], and AC012615.1 has been studied in a prognostic model of glioblastoma [44]. These ndings provided the robust evidence for the construction of our DRLS score based on these 5 lncRNAs.…”

Section: Discussionmentioning

confidence: 80%

Disulfidptosis-related lncRNA signatures in lung adenocarcinoma: Predicting prognosis and evaluating the tumor immune microenvironment

Song,

Cao,

Wang

et al. 2023

Preprint

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Background As a novel form of regulated cell death (RCD), disulfidptosis has been reported recently, which brought the significant probability in better understanding for pathogenesis and therapeutic strategies of tumors. Long non-coding RNAs (LncRNAs) regulate the viability of tumor cells by engaging with a range of targets, including DNA, RNA, and proteins. Nonetheless, the understanding about the prognostic value of disulfidptosis-related LncRNAs (DRlncRNAs) in lung adenocarcinoma (LUAD) remains incomplete. Therefore, our study aimed at establishing a prognostic model for LUAD patients based on DRLncRNAs. Methods RNA-seq data and corresponding clinical information were acquired from The Cancer Genome Atlas (TCGA) database, enabling the identification of DRlncRNAs. Subsequently, a prognostic model was formulated for LUAD by utilizing a series of analyses including univariate COX, LASSO, and multivariate COX regression. Patients were then categorized into two groups with distinct level of DRLS score, and subsequently subjected to the consensus clustering analysis for assigning LUAD patients to distinct subtypes by employing the DRlncRNAs. Subsequent studies investigated disparities among groups with distinct risk and molecular subtypes in terms of overall survival (OS), functional enrichment, the tumor immune microenvironment (TIME), somatic mutations, and drug sensitivity. Finally, in vitro experiments were conducted to validate the LUAD cellular proliferation and migratory behavior upon GSEA knockdown. Results Using the prognostic model consists of 5 DRlncRNAs (AL365181.2, GSEC, AC093673.1, AC012615.1, AL606834.1), the low-risk group exhibited a markedly superior survival in comparison to the high-risk group. The significant differences were observed among patients from different risk groups in OS, immune cell infiltration, immune checkpoint expression, immunotherapy response, and mutation landscape. Experimental results from cellular studies demonstrate the knockdown of lncRNA GSEC leading to a significant reduction in the proliferation and migration abilities of LUAD cells. Conclusion Our prognostic model, constructed using 5 DRlncRNAs, exhibited the capacity to independently predict the survival of LUAD patients, providing the potentially significant assistance in prognosis prediction, and treatment effects optimization. Moreover, our study established a foundation for further research on disulfidptosis in LUAD and proposed new perspectives for the treatment of LUAD.

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“…It has been found that lncRNA LINC01057 can promote the invasion and radio‐resistance of GBM cells, as well as inducing EMT by promoting the nuclear translocation of IKKα to activate the NF‐κB signalling pathway 87 . Yang et al 88 constructed EMT‐related lncRNA prognostic signatures for patients with GBM, and confirmed that EMT and metastasis‐related pathways are risk indicators for patients with GBM. Moreover, by exploiting invasion‐related lncRNAs from single‐cell RNA sequencing data, it was found that patients with GBM exhibiting high NEAT1 expression had poor OS and DFS, and could promote the occurrence and progression of the malignant phenotype in patients with GBM 89 …”

Section: Long Non‐coding Rnasmentioning

confidence: 99%

Systematic characterization and biological functions of non‐coding RNAs in glioblastoma

et al. 2022

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Glioblastoma multiforme (GBM) is the most malignant and aggressive type of glioma. Non‐coding RNAs (ncRNAs) are RNAs that do not encode proteins but widely exist in eukaryotic cells. The common characteristics of these RNAs are that they can all be transcribed from the genome without being translated into proteins, thus performing biological functions, particularly microRNAs (miRNAs), long non‐coding RNAs (lncRNAs) and circular RNAs. Studies have found that ncRNAs are associated with the occurrence and development of GBM, and there is a complex regulatory network among ncRNAs, which can regulate cell proliferation, migration, apoptosis and differentiation, thus provide a basis for the development of highly specific diagnostic tools and therapeutic strategies in the future. The present review aimed to comprehensively describe the biogenesis, general features and functions of regulatory ncRNAs in GBM, and to interpret the potential biological functions of these ncRNAs in GBM as well as their impact on clinical diagnosis, treatment and prognosis and discusses the potential mechanisms of these RNA subtypes leading to cancer in order to contribute to the better design of personalized GBM therapies in the future.

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Identification of an epithelial-mesenchymal transition-related lncRNA prognostic signature for patients with glioblastoma

Cited by 12 publications

References 45 publications

A circular RNA derived from GLIS3 accelerates the proliferation of glioblastoma cells through competitively binding with miR-449c-5p to upregulate CAPG and GLIS3

A circular RNA derived from GLIS3 accelerates the proliferation of glioblastoma cells through competitively binding with miR-449c-5p to upregulate CAPG and GLIS3

Disulfidptosis-related lncRNA signatures in lung adenocarcinoma: Predicting prognosis and evaluating the tumor immune microenvironment

Systematic characterization and biological functions of non‐coding RNAs in glioblastoma

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