Identification of an oncofetal antigen (gp90) on murine B16 melanoma cells

Nishio, Chieko; Ishii, Yoshifumi; Ishii, Keita; Kikuchi, Kokichi

doi:10.1016/0277-5379(82)90228-0

Cited by 3 publications

(2 citation statements)

References 9 publications

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“…A number of B16 melanoma-associated surface markers have now been defined with antisera and MoAbs [2,6,7,8,11,12,16,23,[27][28][29][30]. Molecular weight estimates of molecules defined in those studies have ranged from 20-375 kD, with most in the 40-100kD range.…”

Section: Discussionmentioning

confidence: 99%

Synthesis and expression of metastasis-associated, Met-72/83 antigens

1988

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In the present study, we report a more detailed biochemical analysis of the B16 melanoma, metastasis-associated, Met-72 antigen. Specifically, we have examined (1) the molecular forms of Met-72 isolated during synthesis, surface expression and 'shedding' and (2) the cell-surface expression of Met-72 during the cell cycle. These experiments show that the 72 kD species originally described has an isoelectric point of between 6.3 and 6.9, but is the desialylated derivative of an 83 kD native molecule whose isoelectric point ranges between pH 4.9 and 5.6. In addition, a 90 kD glycoprotein doublet was immunoprecipitated from biosynthetically labelled B16 melanoma cells, but does not appear to be a precursor of the 83 kD or 72 kD molecule. These findings have led us to interchangeably use the terminology Met-72 and Met 72/83. The latter terminology more accurately describes the physical forms which can be identified by different labelling procedures. When culture supernatants from 3H-leucine labelled cells were subjected to anti-Met-72 immunoprecipitation, a 35 kD species was identified as a possible 'shed' product of these cells. Met-72/83 expression during the cell cycle was analyzed by flow cytometry and found not to be restricted to any particular stage. In addition, experiments were performed to determine whether low levels of Met-72 expression on poorly metastatic B16 melanomal clones was a direct result of low levels of synthesis, or if other control mechanisms regulated intracellular pools of Met-72 prior to cell-surface expression.

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Section: Discussionmentioning

confidence: 99%

Synthesis and expression of metastasis-associated, Met-72/83 antigens

1988

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“…These antibodies have been used, in turn, to examine the quantities and distributions of gp90 on tumor and normal cell lines and tissues of fetal and adult origin. Examination of various B16 melanoma cell lines by immunoprecipitation and ELISA assays indicated that gp90 is expressed on melanoma cells in the following relative amounts from highest to lowest: Since Nishio et al (27) reported that a melanoma-associated oncofetal antigen of M T -90 000 is expressed on melanoma cells as well as on some carcinoma, myeloma, leukemia cells, and on fetal fibroblasts, we examined the relative amounts and tissue distributions of gp90 using peroxidase-labeled antibodies. Fetal mouse tissues showed little reactivity with anti-gp90 until the 19th day of development, when gut epithelium stained with the anti-gp90 reagents.…”

Section: Cell Surface Glycoproteins and Brain Metastasismentioning

confidence: 99%

Brain surface invasion and metastasis of murine malignant melanoma variants

Nicolson

Kawaguchi

et al. 1987

J Neuro-Oncol

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Mouse B16 melanoma sublines were selected sequentially for their abilities to colonize brain meninges and leptomeninges of C57BL/6 mice. After 14 selections subline B16-B14b was established that formed significantly more brain tumor colonies than the parental B16 line. Examination of brains at various times after intravenous or intra-arterial injection of B16 cells by electron microscopy revealed that B14b melanoma cells lodged in small brain blood vessels, proliferated and invaded through vessel walls into brain parenchyma and also along small blood vessels at perivascular sites. Invasion into brain parenchyma was characterized by extension of melanoma cell filopodia resulting in fragmentation and sometimes enfulgment of glial and neural cells. Analysis of cell surface proteins of B16 melanoma sublines revealed increased exposure of a Mr approximately 90,000 glycoprotein on the high brain-colonizing cells. Antibodies against the Mr approximately 90,000 glycoprotein reacted with a variety of human melanoma cell lines and with some fetal and adult tissues, indicating that this melanoma-associated component is not species-, tumor- or tissue-specific. The glycoprotein could be a cell surface receptor important in the survival and growth properties of melanoma cells in brain microenvironments.

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Detection of S-100 protein in melanocytic and neurogenic cutaneous tumors

et al. 1984

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Quantitative determination of S-100 protein content on human melanoma tissues and in sera of melanoma patients was made possible through radioimmunoassay (RIA). Melanoma tissue extracts prepared from metastatic sites in both skin and lymph nodes contained 0.08-2.80% of S-100 out of the total extractable proteins, and it was also noticed that the tumors obtained from lymph-node metastasis possessed slightly higher levels of S-100 as compared with those obtained from the skin lesions. Serum S-100 levels of 12 melanoma patients with Stage 1 to 3 diseases were also examined by means of RIA, but only two patients showed a slight elevation of serum S-100 levels. When tissue sections prepared from a variety of cutaneous tumors, including pigmented nevus, melanoma and Schwannoma, were stained for S-100 by an immunoperoxidase technique, these tumors of neuroectodermal origin were demonstrated to contain a detectable amount of S-100, whereas other tested nonmelanocytic and non-neurogenic tumors were entirely negative for S-100. These data stress the usefulness of S-100 for the diagnosis of neurogenic and melanocytic tumors, especially melanomas, despite the difficulty of its detection in the sera of the tumor bearers, which might reflect the low tendency of S-100 to be shed from the tumor cells.

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Identification of an oncofetal antigen (gp90) on murine B16 melanoma cells

Cited by 3 publications

References 9 publications

Synthesis and expression of metastasis-associated, Met-72/83 antigens

Synthesis and expression of metastasis-associated, Met-72/83 antigens

Brain surface invasion and metastasis of murine malignant melanoma variants

Detection of S-100 protein in melanocytic and neurogenic cutaneous tumors

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