Identification of an SCF ubiquitin-ligase complex required for auxin response in Arabidopsis thaliana

Gray, William M.; Pozo, Juan C.; Walker, Loni M.; Hobbie, Lawrence; Risseeuw, Eddy; Banks, Travis; Crosby, William L.; Yang, Ming; Mā, Hong; Estelle, Mark

doi:10.1101/gad.13.13.1678

Cited by 471 publications

(526 citation statements)

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“…Generally, F-box proteins interact with the CUL1 -RBX1 subcomplex through the ASK1 or ASK2 proteins. TIR1 immunoprecipitates with CUL1, ASK1 and RBX1, indicating that these subunits form an SCF TIR1 complex [15,17]. In line with this, the tir1, ask1 and axr6 mutants are resistant to auxin [15,18].…”

Section: Auxin Modulates Gene Transcriptionmentioning

confidence: 70%

“…Auxin signaling is mediated through the ubiquitinproteasome pathway In Arabidopsis, the characterization of the auxin resistant mutants axr1 and tir1 led to the discovery that the ubiquitin-proteasome pathway is involved in auxin response [12,14,15]. Ubiquitin-mediated protein degradation has emerged as a vital process that regulates the growth and development of eukaryotic organisms.…”

Section: Auxin Modulates Gene Transcriptionmentioning

confidence: 99%

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Auxin signaling and regulated protein degradation

Dharmasiri

Estelle

2004

Trends in Plant Science

233

141

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Auxin regulates many aspects of plant growth and development. Auxin is known to regulate gene expression through degradation of the AUX/IAA proteins, short-lived nuclear proteins that regulate gene transcription. The AUX/IAA proteins are degraded through the action of a ubiquitin protein ligase called SCF TIR1 . Auxin promotes the interaction between AUX/IAA proteins and SCF TIR1 in a soluble extract, suggesting that the auxin receptor is a soluble factor. These studies also indicate that the auxin-induced AUX/IAA -SCF TIR1 interaction does not depend on phosphorylation or hydroxylation of AUX/IAA proteins. Although the mechanism of auxin-induced AUX/IAA -SCF TIR1 interaction is not yet clear, auxin might induce a modification in TIR1, AUX/IAA proteins or an adaptor protein that is required for the interaction.Auxin plays a pivotal role in many processes throughout the plant life cycle. These include embryogenesis, lateral root development, vascular differentiation, apical dominance, tropic responses and flower development. Auxin was first discovered decades ago [1]. In spite of the tremendous amount of information that has accumulated since then, the auxin signaling pathways have not been fully elucidated. Nevertheless, through the combined application of genetic, molecular and biochemical methods, we are beginning to understand some aspects of auxin action. In this article, we discuss recent developments in the study of auxin signaling, particularly focusing on auxin-regulated transcription.

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Section: Auxin Modulates Gene Transcriptionmentioning

confidence: 70%

Section: Auxin Modulates Gene Transcriptionmentioning

confidence: 99%

Auxin signaling and regulated protein degradation

Dharmasiri

Estelle

2004

Trends in Plant Science

233

141

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“…We carried out immunoprecipitation assays as described 28 using A. thaliana protein extracts prepared from 12-d-old to 14-d-old seedlings grown under long-day photoperiod conditions in solid nutrient medium. We prepared extracts by homogenizing seedlings in ice-cold buffer containing 50 mM Tris Cl, 150 mM NaCl, 0.5% Nonidet P-40, 1 mM phenylmethylsulfonyl fluoride and 1× protease inhibitor cocktail.…”

Section: Methodsmentioning

confidence: 99%

Regulation of flowering time by FVE, a retinoblastoma-associated protein

et al. 2004

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The initiation of flowering in plants is controlled by environmental and endogenous signals 1,2 . Molecular analysis of this process in Arabidopsis thaliana indicates that environmental control is exerted through the photoperiod and vernalization pathways, whereas endogenous signals regulate the autonomous and gibberellin pathways. The vernalization and autonomous pathways converge on the negative regulation of FLC 3,4 , a gene encoding a MADS-box protein that inhibits flowering 3,4 . We cloned FVE, a component of the autonomous pathway that encodes AtMSI4, a putative retinoblastomaassociated protein. FVE interacted with retinoblastoma protein in immunoprecipitation assays, and FLC chromatin was enriched in acetylated histones in fve mutants. We conclude that FVE participates in a protein complex repressing FLC transcription through a histone deacetylation mechanism. Our data provide genetic evidence of a new developmental function of these conserved proteins and identify a new genetic mechanism in the regulation of flowering. shown in the upper part, capital letters correspond to the wild-type or mutated nucleotides. In the protein schematic, gray boxes represent WD repeats, the narrow black box represents a putative nuclear localization signal and the asterisk indicates a putative retinoblastoma-binding motif.

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“…Recent genetic and biochemical analyses have suggested that ubiquitin-related proteolysis is central to several aspects of auxin response (Gray et al, 1999(Gray et al, , 2001Dharmasiri and Estelle, 2002;Kepinski and Leyser, 2002;Leyser, 2002). As a model system, some advances in how auxin promotes lateral root formation have been reported recently (Xie et al, 2000;Casimiro et al, 2001;Xie et al, 2002).…”

Section: Introductionmentioning

confidence: 99%