Identification of antibacterial constituents from the indigenous Australian medicinal plant Eremophila duttonii F. Muell. (Myoporaceae)

Smith, John Grieve; Tucker, David J.; Watson, Kenneth; Jones, Graham P.

doi:10.1016/j.jep.2007.03.031

Cited by 51 publications

(48 citation statements)

References 20 publications

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“…Serrulatane quinonoid biflorin, which is isolated from the root tissue of Capraria biflora , has been reported to exhibit antimicrobial activities against Gram-positive bacteria [38]. This activity is consistent with the traditional use to treat conjunctivitis, which may be associated with infections by S. aureus , S. epidermidis and Propionibacterium spp.…”

Section: Resultsmentioning

confidence: 62%

A screening for antimicrobial activities of Caribbean herbal remedies

Luciano-Montalvo

Boulogne

Gavillán-Suárez

2013

BMC Complement Altern Med

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BackgroundThe TRAMIL program aims to understand, validate and expand health practices based on the use of medicinal plants in the Caribbean, which is a “biodiversity hotspot” due to high species endemism, intense development pressure and habitat loss. The antibacterial activity was examined for thirteen plant species from several genera that were identified as a result of TRAMIL ethnopharmacological surveys or were reported in ethnobotanical accounts from Puerto Rico. The aim of this study was to validate the traditional use of these plant species for the treatment of bacterial infections, such as conjunctivitis, fever, otitis media and furuncles.MethodsAn agar disc diffusion assay was used to examine five bacterial strains that are associated with the reported infections, including Staphylococcus saprophyticus (ATCC 15305), S. aureus (ATCC 6341), Escherichia coli (ATCC 4157), Haemophilus influenzae (ATCC 8142), Pseudomonas aeruginosa (ATCC 7700) and Proteus vulgaris (ATCC 6896), as well as the fungus Candida albicans (ATCC 752). The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values were determined for each of the extracts that showed inhibitory activity.ResultsThe decoctions of Pityrogramma calomelanos, Tapeinochilus ananassae, and Syzygium jambos, as well as the juice of Gossypium barbadense, showed > 20% growth inhibition against several bacteria relative to the positive control, which was the antibiotic Streptomycin. Extracts with the best antimicrobial activities were S. jambos that showed MIC = 31 μg/mL and MBC = 1.0 mg/mL against P. vulgaris and T. ananassae that showed MIC = 15 μg/mL against S. aureus.ConclusionThis report confirms the traditional use of P. calomelanos for the treatment of kidney infections that are associated with stones, as well as the antimicrobial and bactericidal effects of T. ananassae against P. vulgaris and S. saprophyticus and the effects of S. jambos against S. aureus and S. saprophyticus.

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Section: Resultsmentioning

confidence: 62%

A screening for antimicrobial activities of Caribbean herbal remedies

Luciano-Montalvo

Boulogne

Gavillán-Suárez

2013

BMC Complement Altern Med

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“…Bioautography was performed based on the method of Smith et al (2007) with minor modifications. Briefly, 30 μL (300 μg) of the stock extract (10 mg mL −1 ) was spotted onto silica gel 60F 254 aluminium thin-layer chromatography (TLC) plates (Merck, Germany), together with 30 μL of ethyl acetate as a negative control and 0.5 μg of novobiocin (Sigma, Ireland) as a positive control.…”

Section: Methodsmentioning

confidence: 99%

“…Both disc diffusion and bioautography are qualitative measurements that indicate presence or absence of antibacterial agents, while the broth dilution method is a quantitative assay used for assessment of the minimum inhibitory concentration (MIC) (Rios et al 1988;Silva et al 2005;Valgas et al 2007;Wikler et al 2009a). The disc diffusion assay is simple and practical and is the most commonly used method for antimicrobial screening recommended by the Clinical and Laboratory Standards Institute (CLSI) (Wikler et al 2009b) while bioautography facilitates a more comprehensive investigation of the extract through a combination of sub-fractionation coupled with visualization of antibacterial efficacy of the fractions (Martini and Eloff 1998;Smith et al 2007). Ulva lactuca (Ulvophyceae; Ulvaceae), commonly known as 'sea lettuce', is found in abundance in Ireland and can be a nuisance in nutrient-rich areas such as harbours or ports.…”

Section: Introductionmentioning

confidence: 99%

Extraction and bioautographic-guided separation of antibacterial compounds from Ulva lactuca

et al. 2011

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This study aimed to optimize an extraction and separation procedure to obtain a concentrated fraction with antibacterial activity from the macroalga Ulva lactuca. Antibacterial compounds were extracted using eight solvents, and consistent activity against Staphylococcus aureus, Bacillus subtilis and methicillin-resistant (MR) S. aureus was observed from a dilute (1:100, w/v) ethyl acetate extract. Seasonal analysis revealed that antibacterial activity was the lowest in spring/summer and the highest in autumn/winter. Bioautography was found to be a more appropriate assay compared to disc diffusion when screening crude extracts, as it separates the masking compounds from the antibacterial compounds and a direct assessment of the bands responsible for the antibacterial effect could be made. The antibacterial compounds were first separated from the crude extract using preparative thin-layer chromatography, followed by column chromatography to obtain a semi-pure sub-fraction. Using this approach, the antibacterial compounds were successfully concentrated from a crude extract (300 μg) to semi-pure fractions (6 μg) in which antibacterial activities were greatly enhanced. This study also revealed that prolonged storage (9 months) under a nitrogen atmosphere at −20°C resulted in a considerable increase in antibacterial activity. This is the first report of seasonal assessment of antibacterial compounds from seaweeds collected in Ireland. In addition, an antibacterial fraction was successfully isolated from U. lactuca which exhibited potent anti-MR S. aureus activity.

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“…Further, the cuticle has antibiotic properties and helps to prevent organ fusions (Luo et al, 2007;Smith et al, 2007;Weng et al, 2010). Thus, it is a structure vital for a plant´s survival on land.…”

Section: Discussionmentioning

confidence: 99%

Plant lipid transfer proteins: Evolution, expression and function

Edstam¹

2013

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Identification of antibacterial constituents from the indigenous Australian medicinal plant Eremophila duttonii F. Muell. (Myoporaceae)

Cited by 51 publications

References 20 publications

A screening for antimicrobial activities of Caribbean herbal remedies

A screening for antimicrobial activities of Caribbean herbal remedies

Extraction and bioautographic-guided separation of antibacterial compounds from Ulva lactuca

Plant lipid transfer proteins: Evolution, expression and function

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