Identification of apple cultivars using RAPD markers

Koller, Bernhard; Lehmann, Anton; McDermott, J. M.; Gessler, C.

doi:10.1007/bf00225036

Cited by 173 publications

(83 citation statements)

References 11 publications

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“…This confirms the usefulness of RAPD markers for fingerprinting purposes suggested in many previous studies (e.g. Koller et at., 1993;Keil & Griffin, 1994).…”

Section: Discussionsupporting

confidence: 91%

Empirical assessment of allozyme and RAPD variation in Pinus sylvestris (L.) using haploid tissue analysis

Szmidt

Wang

1996

Heredity

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We analysed 20 allozyme and 22 putative random amplified polymorphic DNA (RAPD) loci in two populations of Pinus sylvestris (L.) from northern Sweden. Genotypes for individual allozyme and RAPD loci were inferred from segregation patterns in haploid macrogametophytes. Therefore, it was possible to distinguish between homo-and heterozygotes carrying a RAPD fragment and to estimate directly the frequencies of RAPD fragments. The percentage of polymorphic loci and the expected and observed heterozygosity were lower for allozymes than for RAPDs. Average fixation indices for both types of markers were negative indicating a heterozygote excess over panmictic expectations. The apportionment of genetic variation within and among the investigated populations was similar for allozymes and RAPDs and showed that most of the variation resided within populations. RAPD genotypes inferred from haploid material were subsequently converted to diploid phenotypes and used to estimate indirectly the frequencies of RAPD fragments. Gene diversity measurements derived from indirectly estimated RAPD frequencies were distinctly lower than those based on directly estimated RAPD frequencies. This result was caused by the absence of the null homozygote at many loci which appeared as monomorphic in the diploid data set. Population differentiation coefficients based on the indirectly estimated RAPD frequencies were not concordant with those based on directly estimated RAPD frequencies. Our present results indicate that when complete genotype information can be obtained, RAPD analysis provides genetic information similar to that revealed by analysis of allozyme variation. On the other hand, our results are concordant with theoretical results suggesting that analysis of RAPD variation in diploid material can produce unreliable estimates of population-genetic parameters.

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“…This confirms the usefulness of RAPD markers for fingerprinting purposes suggested in many previous studies (e.g. Koller et at., 1993;Keil & Griffin, 1994).…”

Section: Discussionsupporting

confidence: 91%

Empirical assessment of allozyme and RAPD variation in Pinus sylvestris (L.) using haploid tissue analysis

Szmidt

Wang

1996

Heredity

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“…[147,159] This could also be used in the study of genotype identification. [160][161][162] In another study, Sasaki and Nagumo reported rapid identification of C. longa and C. aromatica by LAMP method. [163] A study conducted by Sigrist et al showed genetic diversity of turmeric germplasm (C. longa; Zingiberaceae) using microsatellite markers to discover genetics and molecular research.…”

Section: Molecular Marker Analysis Inmentioning

confidence: 99%

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Ashraf¹

2017

IJGP

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Curcuma longa Linn. is well-known and valued medicinal plant. It has a long history of traditional uses ranging from folk medicine to several culinary preparations. The phytochemical, pharmacological, and molecular studies of C. longa are reviewed. The rhizome is rich in essential oils, and various numbers of chemical constituents with biomedical significance have been isolated from it. The management of indigenous knowledge by appropriate documentation is recommended. This review was compiled to provide recent consolidated information covering different aspects of the plant, phytochemical, pharmacological, and molecular study to provide a basis on which to plan future studies and to promote sustainable use of C. longa.

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“…Both RAPD and IS-SR marker, based on PCR techniques have proven to be a reliable, easy to generate, inexpensive and versatile set of marker that rely on repeatable amplification of DNA se-quence using single primers. The RAPD and ISSR markers can be used in the study of the genetic variability of species or natural populations and in the identification of genotypes [7][8][9][10][11][12][13][14]. In this communication, we report the feasibility of PCR-based DNA (RAPD and ISSR) marker for phylogeny study and identification for conservation of Phyllanthus species.…”

Section: Introductionmentioning

confidence: 99%