Identification of atranorin and related potential allergens in oakmoss absolute by high-performance liquid chromatography–tandem mass spectrometry using negative ion atmospheric pressure chemical ionization

Lichen extracts as raw materials in perfumery. Part 1: oakmoss

“…[35,36] GC-MS with or without silylation gives good results with mono-aromatic compounds. Obviously, HPLC-MS/MS is the method of choice for the quantitative determination of these compounds, whether it be for measuring large concentrations in industrial extracts [37] or for trace determinations in cosmetic products. [38] Direct analysis of the volatile fraction of an oakmoss extract (an absolute) by GC, for example, is not straightforward if one bothers about the non-volatile fraction, which remains stuck in the chromatographic system, and, consequently, the necessity to use standardization.…”

“…[74] In oakmoss extracts (absolute oils from E. prunastri), the constituents that are known to induce ACD are atranorin 3 and its degradation product, atranol 18, through haematommic acid 26 and chloroatranorin 4 and its degradation product chloroatranol 38 (through chlorohaematommic acid). [34,37,75,76] The alleged identification of 'a second atranol isomer' requires confirmation. [37] As the free haematommic acids are very labile, or because they are mainly present as ethyl esters, which are formed during the processing of the resinoids with ethanol, attention was rather focused on ethyl haematommate 28 and ethyl chlorohaematommate 36.…”

“…[34,37,75,76] The alleged identification of 'a second atranol isomer' requires confirmation. [37] As the free haematommic acids are very labile, or because they are mainly present as ethyl esters, which are formed during the processing of the resinoids with ethanol, attention was rather focused on ethyl haematommate 28 and ethyl chlorohaematommate 36. [22,34] Actually, very little is known about the sensitizing potential of the acids, either isolated or present at low levels, in oakmoss extracts.…”

A liquid chromatography‐mass spectrometric method for the determination of oak moss allergens atranol and chloroatranol in perfumes

“…A liquid chromatography-tandem mass spectrometry (LC-MS-MS) method using negative ion atmospheric pressure chemical ionization (APCI) for the identification of potential allergens in oak moss, including atranol and chloroatranol, has recently been reported [12].This method was focused towards establishing the identity of compounds causing the allergic response, so a quality controlled oak moss with reduced allergenic potential could be prepared. The sensitivity of the method is not known and it has not yet been demonstrated to be applicable for quantification of the allergens.…”

“…The sensitivity of the method is not known and it has not yet been demonstrated to be applicable for quantification of the allergens. In the present study, the method [12] has been further developed and validated for quantitative analysis of atranol and chloroatranol in perfumes and similar products. The method is based on direct injection of the sample and analysis by reverse phase liquid chromatography coupled to tandem mass spectrometry.…”