Identification of autoantibody to melanocytes and characterization of vitiligo antigen in vitiligo patients

Park, Yoon‐Kee; Kim, Nam Soo; Hann, Seung Kyung; Im, Sang Hee

doi:10.1016/0923-1811(95)00427-0

Cited by 32 publications

(15 citation statements)

References 23 publications

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“…Identification was made by immunoprecipitation and autoradiography of radioiodinated cell antigens, a procedure which labels preferentially the cell surface antigens 14 . Park et al 16 . suggested that immunoblotting of unlabeled cell antigens might detect different antigens according to the lysate extraction method used.…”

Section: Discussionmentioning

confidence: 99%

Recognition of melanoma cell antigens with antibodies present in sera from patients with vitiligo

et al. 2000

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Section: Discussionmentioning

confidence: 99%

Recognition of melanoma cell antigens with antibodies present in sera from patients with vitiligo

et al. 2000

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“…92 In immunoblotting studies, melanocyte antigens of 45, 65, 70, 88 and 110 kDa were identified. 94 while, more recently, vitiligo antibodies were demonstrated to recognize melanoma cell proteins of 68, 90 and 165 kDa. 95 Although the identity of these particular antigens has not been confirmed, several specific vitiligo autoantigens have been reported and these are summarized in Table 4.…”

Section: Autoantibodies To Melanocytes In Vitiligomentioning

confidence: 99%

Autoimmunity as an aetiological factor in vitiligo

Rezaei¹,

Gavalas²,

Weetman³

et al. 2007

Acad Dermatol Venereol

147

105

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Vitiligo is a common dermatological disorder characterized by the presence on the skin of depigmented macules resulting from the destruction of cutaneous melanocytes. Autoimmunity is an important hypothesis with regard to vitiligo aetiology and the evidence for autoimmune responses being involved in the pathogenesis of this disorder will be discussed in the present review. All immune system compartments, including innate and adaptive immunity have been implicated in vitiligo development. Particularly relevant are autoantibodies and autoreactive T cells in vitiligo patients that have cytotoxic effects upon pigment cells. Furthermore, predisposition to vitiligo appears to be associated with certain alleles of the major histocompatibility complex class II antigens as well as with other autoimmune-susceptibility genes. Moreover, the association of vitiligo with autoimmune disorders, the animal models of the disease, and the positive response to immunosuppressive therapeutic agents emphasize the role of autoimmunity in the development of this disorder.

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“…The first studies linking vitiligo to autoimmune response involved the detection of serum autoantibodies in vitiligo patients, with autoantibody levels found to be elevated in patients with progressive vitiligo (Park et al, 1996;Kemp et al, 1997b;Li et al, 2000;Lee and Yu, 2004). These antibodies (Abs) mainly target MC Ags including melanosomal membrane-fixed proteins, such as tyrosinase-related proteins 1 and 2 (TYRP-1 and TYRP-2), some structural melanosomal proteins (Kemp et al, 1997a;Kemp et al, 1998a;Kemp et al, 1998bKemp et al, , 1999Okamoto et al, 1998;Palermo et al, 2001), and a 75-kDa membrane protein that had been previously observed but not identified, and named VIT75 (Cui et al, 1992;Cui et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Vitiligo Autoantigen VIT75 Is Identified as Lamin A in Vitiligo by Serological Proteome Analysis Based on Mass Spectrometry

et al. 2011

Journal of Investigative Dermatology

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VIT75 is a 75-kDa melanocyte membrane antigen (Ag) that had been observed, but not identified until now. Its immunopathogenic role in vitiligo remains unknown. In this study, serological proteome analysis based on mass spectrometry was employed to identify VIT75. Three disparate 75-, 60-, and 45-kDa proteins on two-dimensional (2D) gel were, respectively, identified as lamin A, tyrosinase-related protein 1, and melanin-concentrating hormone receptor 1. The latter two proteins are well-known Ags. Immunoreactivity analysis showed that the 75-kDa protein displayed on the 2D gel was recognized by human anti-lamin A IgG. Antibody (Ab) reactivity to lamin A was positive in 28.6% of patients' sera. Only 3.1% healthy sera reacted with the lamin A. A total of 91.7% of the positive sera was from the active non-segmental vitiligo (NSV). The positive rate and mean titer of anti-lamin A Ab are higher for NSV with autoimmune disease than for NSV without autoimmune disease. These data demonstrate that VIT75 is lamin A. To our knowledge, this is a previously unreported vitiligo Ag. Anti-lamin A Ab may be a potential marker of NSV with autoimmune disease. The study indicates that the targets of autoantibodies in vitiligo patients can be revealed by serological proteome analysis.

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Identification of autoantibody to melanocytes and characterization of vitiligo antigen in vitiligo patients

Cited by 32 publications

References 23 publications

Recognition of melanoma cell antigens with antibodies present in sera from patients with vitiligo

Recognition of melanoma cell antigens with antibodies present in sera from patients with vitiligo

Autoimmunity as an aetiological factor in vitiligo

Vitiligo Autoantigen VIT75 Is Identified as Lamin A in Vitiligo by Serological Proteome Analysis Based on Mass Spectrometry

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