Identification of Azadirachtin in Tissue-Cultured Cells of Neem (Azadirachta Indica)

Analysis of small samples of limonoids of neem (Azadirachta indica) using solid phase extraction from tissue culture

“…In the course of our collaborative work (Allan et al, 1994;Jarvis et al, 1997) concerning the tissue culture of dispersed neem cells, many small samples of dried tissue have been generated and it was required to have information about the kinds and amounts of limonoids, especially 1, being accumulated therein (Jarvis et al, 1997). We describe here a system that has been devised for the analysis of limonoids in neem using the principle of SPE for the separation of the initial mixture into fractions in which individual compounds can then be quantified by SFC or HPLC.…”

“…EXPERIMENTAL Initial solvent extraction. Cell cultures of callus tissue, suspension and hairy root cultures that had been grown on a modified Murashige and Skoog (Jarvis et al, 1997) medium were used. Freeze-dried samples (0.5-1.0 g) of neem cell cultures were ground under liquid nitrogen in a mortar to break down the cells.…”

Neem: Today and in the New Millennium

“…These suspension cultures were initiated from callus cultures induced from seedling hypocotyls (seeds originated from Togo). A sure chemically acceptable proofs that azadirachtin A was produced in suspension cultures of neem was reported by Jarvis et al (1997). Other compounds also produced by the suspension cultures were azadirachtin I, nimbin, 6desacetylnimbin, salannin, 3-desacetylsalannin, 3-tigloylazadirachtol (azadirachtin B) and 3-acetyl-1-tigloylazadirachtinin.…”

Neem Plant Tissue Culture Today and the Outlook for the New Millennium

“…These suspension cultures were initiated from callus cultures induced from seedling hypocotyls (seeds originated from Togo). A sure chemically acceptable proofs that azadirachtin A was produced in suspension cultures of neem was reported by Jarvis et al (1997). Other compounds also produced by the suspension cultures were azadirachtin I, nimbin, 6-desacetylnimbin, salannin, 3-desacetylsalannin, 3-tigloylazadirachtol (azadirachtin B) and 3-acetyl-1-tigloylazadirachtinin.…”