Identification of Bacteria in Soil by MALDI-TOF MS and Analysis of Bacillus spp., Paenibacillus spp. and Pseudomonas spp. with PCA

Cevik, Yasemin Numanoglu; Öğütçü, Hatice

doi:10.1080/22297928.2021.1877194

Cited by 9 publications

(6 citation statements)

References 19 publications

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“…The three (3) bacterial isolates were identified using Matrix assisted laser desorption ionisation time of flight mass spectrometry (MALDI-TOF MS). This method of bacterial identification involves mass spectrometry and was carried out in a MALDI-TOF MS device (Bruker Corporation, MA, Billerica, USA) using Flex control software (version 3.4, build 135.10) [ 36 ]. The database used to identify strains was the MBT Compass Library revision K, version 12 (2022).…”

Section: Methodsmentioning

confidence: 99%

Effect of the Application of Ochrobactrum sp.-Immobilised Biochar on the Remediation of Diesel-Contaminated Soil

Dike,

Rani Batra,

Khudur

et al. 2024

Toxics

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The immobilisation of bacteria on biochar has shown potential for enhanced remediation of petroleum hydrocarbon-contaminated soil. However, there is a lack of knowledge regarding the effect of bacterial immobilisation on biosolids-derived biochar for the remediation of diesel-contaminated soil. This current study aimed to assess the impact of the immobilisation of an autochthonous hydrocarbonoclastic bacteria, Ochrobacterium sp. (BIB) on biosolids-derived biochar for the remediation of diesel-contaminated soil. Additionally, the effect of fertiliser application on the efficacy of the BIB treatment was investigated. Biochar (BC) application alone led to significantly higher hydrocarbon removal than the control treatment at all sampling times (4887–11,589 mg/kg higher). When Ochrobacterium sp. was immobilised on biochar (BIB), the hydrocarbon removal was greater than BC by 5533 mg/kg and 1607 mg/kg at weeks 10 and 22, respectively. However, when BIB was co-applied with fertiliser (BIBF), hydrocarbon removal was lower than BIB alone by 6987–11,767 mg/kg. Quantitative PCR (q-PCR) analysis revealed that the gene related to Ochrobacterium sp. was higher in BIB than in the BC treatment, which likely contributed to higher hydrocarbon removal in the BIB treatment. The results of the q-PCR analysis for the presence of alkB genes and FTIR analysis suggest that the degradation of alkane contributed to hydrocarbon removal. The findings of this study demonstrate that bacterial immobilisation on biosolids-derived biochar is a promising technique for the remediation of diesel-contaminated soil. Future studies should focus on optimising the immobilisation process for enhanced hydrocarbon removal.

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Section: Methodsmentioning

confidence: 99%

Effect of the Application of Ochrobactrum sp.-Immobilised Biochar on the Remediation of Diesel-Contaminated Soil

Dike,

Rani Batra,

Khudur

et al. 2024

Toxics

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“…Laser pulses consisting of 40 packets of 240 were applied to measure each colony. 26 To ensure accuracy, each sample was studied in triplicate, providing multiple spectra for analysis.…”

Section: Methodsmentioning

confidence: 99%

Exploring stress-tolerant plant growth-promoting rhizobacteria from groundnut rhizosphere soil in semi-arid regions of Ethiopia

Beshah,

Muleta,

Legese

et al. 2024

Plant Signaling & Behavior

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“…A 60 Hz nitrogen laser was employed at 337 nm as the ion source. To obtain the spectra, laser pulses consisting of 40 packets of 240 were applied in the measurement of each colony [ 20 ]. Each sample was studied in triplicate.…”

Section: Methodsmentioning

confidence: 99%

In Vitro Evaluation of Extracellular Enzyme Activity and Its Biocontrol Efficacy of Bacterial Isolates from Pepper Plants for the Management of Phytophthora capsici

Admassie

Wolde-Hawariat

Alemu

2022

BioMed Research International

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Phytophthora capsici is one of the most devastating fungal pathogens, causing severe diseases that lead to economic loss in the pepper industry. As a result of the infections, the chemical approach is becoming more popular. Biological control, on the other hand, is better suited to controlling fungal pathogens. The biological control approach significantly reduces the problems associated with chemical applications while restoring natural environmental balance. As a result, the overall findings indicate that certain bacterial isolates play a beneficial role in lytic enzyme production and biocontrol activities against P. capsici. Bacterial isolates obtained from the pepper plants were screened for lytic enzyme and anti-oomycete activity against Phytophthora capsici in Ethiopia. Sixty bacterial isolates were isolated and tested against Phytophthora capsici. From these bacterial isolates, different inhibition zones and hydrolytic enzyme production were detected. Biochemical tests using an automated machine (MALDI-TOF, VITEK 2 compact and 16S rRNA) revealed that three of them, AAUSR23, AAULE41, and AAULE51, showed a high inhibition zone and high production of hydrolytic enzymes and were identified as Enterobacter cloacae (AAUSR23), Pseudomonas fluorescens (AAULE41), and undetermined (AAULE51). The effects of diffusable metabolite isolate AAULE51 has a 66.7% inhibition zone against Phytophthora capsici, followed by AAULE41 and AAUSR23, which have 59.7% and 14.1% inhibition zones, respectively. These bacterial isolates showed high production of hydrolytic enzymes like protease, cellulase, chitinase, and lipase (5-34 diameter of inhibition zone). As a result, the overall findings show that selected bacterial isolates play a beneficial role in lytic enzyme production and for their biocontrol activities against P. capsici.

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Identification of Bacteria in Soil by MALDI-TOF MS and Analysis of Bacillus spp., Paenibacillus spp. and Pseudomonas spp. with PCA

Cited by 9 publications

References 19 publications

Effect of the Application of Ochrobactrum sp.-Immobilised Biochar on the Remediation of Diesel-Contaminated Soil

Effect of the Application of Ochrobactrum sp.-Immobilised Biochar on the Remediation of Diesel-Contaminated Soil

Exploring stress-tolerant plant growth-promoting rhizobacteria from groundnut rhizosphere soil in semi-arid regions of Ethiopia

In Vitro Evaluation of Extracellular Enzyme Activity and Its Biocontrol Efficacy of Bacterial Isolates from Pepper Plants for the Management of Phytophthora capsici

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