Identification of bacterial protein markers and enolase as a plant response protein in the infection of Olea europaea subsp. europaea by Pseudomonas savastanoi pv. savastanoi

Abstract

Olive knot disease is characterised by the development of galls on Olea europaea stems as a result of infection by Pseudomonas savastanoi pv. savastanoi. Protein differential accumulation during the first week of infection was studied using twodimensional gel electrophoresis and MALDI-TOF mass spectrometry to investigate the biochemical changes occurring in infected tissues and to understand the factors involved in bacteria pathogenesis and plant response to infection. Common infection symptoms were obtained in 1 year-old plants of two Portuguese cultivars, 'Galega' and 'Cordovil de Serpa' using the strain NCPPB 2327. The comparison of protein patterns of non-inoculated stem tissues, stem tissues inoculated with water or with the strain NCPPB 2327 led to the detection of differentially expressed infection-related proteins. Moreover a distinct protein pattern was obtained between cultivars in response to infection. The differential protein expression was characterised by qualitative and quantitative variation. Among the differentially expressed proteins were the bacterial P. savastanoi orthologues of outer membrane porin F, the tellurium resistance protein, aconitate hydratase 2 and a hypothetical protein with unknown function. From O. europaea, protein orthologues of enolase and calcium-dependent protein kinase were found to be differentially expressed. Results are discussed in the context of the molecular basis of plant-pathogen interactions in the search for markers for the presence of the bacterium in plant tissues.

Comparative proteomic analysis in pea treated with microbial consortia of beneficial microbes reveals changes in the protein network to enhance resistance against Sclerotinia sclerotiorum

“…Its increased levels were also observed in response to plant pathogens P. savastanoi and Fusarium graminearum (Campos et al, 2009;Yang et al, 2010). It's up-regulation was also observed in all the challenged plants treated with BCAs either singly or in the form of consortia in comparison to challenged control plants.…”

“…europaea upon P. savastanoi pv. savastanoi infection which may be due to a change in bacterial metabolism that leads to an up-regulation of the enzymes of the aerobic respiratory pathway for energy production (Campos et al, 2009).…”

Early physiological and biochemical responses of rice seedlings to low concentration of microcystin-LR

“…After IEF, the gel strips were equilibrated using 10 mg/ mL dithiothreitol followed by 25 mg/mL iodoacetamide in urea (6 M), glycerol (30 %, v/v), SDS (2 %, w/v) as described in Campos et al (2009). This procedure reduces and alkylates the proteins.…”

Plant-bacterium interactions analyzed by proteomics

“…campestris in association with B. oleracea and Pseudomonas savastanoi pv. savastanoi resulted in comprehensive expression analysis including stress and metabolic proteins (Andrade et al, 2008; Campos et al, 2009). Increased levels of proteins involved in xanthan biosynthesis, stress response, and the metabolism were induced in X. campestris in planta conditions compared with in vitro grown cells (Andrade et al, 2008).…”

“…Proteins differentially expressed in response to P. savastanoi pv. savastanoi on O. europaea stems were related with photosynthesis and metabolism (Campos et al, 2009). Non-pathogenic bacteria ( P. putida ) had been reported to promote systemic resistance in different host plants as well.…”