Identification of Bartonella (Rochalimaea) species among fastidious gram-negative bacteria on the basis of the partial sequence of the citrate-synthase gene

Joblet, Christine; Roux, Véronique; Drancourt, Michel; Gouvernet, Joanny; Raoult, Didier

doi:10.1128/jcm.33.7.1879-1883.1995

Cited by 63 publications

(24 citation statements)

References 33 publications

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“…The presence of the genes encoding the citrate synthase and the 16S to 23S ribosomal RNA intergenic spacer region was determined by PCR using previously described protocols. 12,13 In addition, we extracted the DNA from 200 µL of patients' serum, when available, using the QIAamp blood kit (QIAGEN). Bartonella species were detected from serum using a nested PCR assay that incorporated primers derived from the riboflavin synthase-encoding gene (Zaher Zeaiter, P.-E.F., Gilbert Greub, MD, D.R., unpublished data, 2002).…”

Section: Microbiological and Molecular Methodsmentioning

confidence: 99%

Outcome and Treatment of Bartonella Endocarditis

Raoult¹,

Fournier²,

Vandenesch³

et al. 2003

Arch Intern Med

216

147

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Section: Microbiological and Molecular Methodsmentioning

confidence: 99%

Outcome and Treatment of Bartonella Endocarditis

Raoult¹,

Fournier²,

Vandenesch³

et al. 2003

Arch Intern Med

216

147

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“…DNA extracts were prepared from suspect colonies or from immunofluorescence-positive shell vials for use as templates in PCR amplification with the QIAmp blood kit (Qiagen, Hilden, Germany), according to the manufacturer's instructions. During the first 3 years of this study, molecular detection and identification were based on 16S rRNA gene and citrate synthase gene amplification and sequencing as previously described (26,53). Subsequently, a new procedure for citrate synthase gene amplification was designed.…”

Section: Methodsmentioning

confidence: 99%

Culture of Bartonella quintana and Bartonella henselae from Human Samples: a 5-Year Experience (1993 to 1998)

1999

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Bartonella quintana and Bartonella henselaeare fastidious gram-negative bacteria responsible for bacillary angiomatosis, trench fever, cat scratch disease, and endocarditis. During a 5-year period, we received 2,043 samples for culture ofBartonella sp. We found Bartonella sp. to be the etiologic agent in 38 cases of endocarditis, 78 cases of cat scratch disease, 16 cases of bacteremia in homeless people, and 7 cases of bacillary angiomatosis. We correlated the results of positive cultures with the clinical form of the disease, type of sample, culture procedure, PCR-based genomic detection, and antibody determination. Seventy-two isolates of B. quintana and nine isolates ofB. henselae from 43 patients were obtained. Sixty-three of the B. quintana isolates and two of the B. henselae isolates, obtained from patients with no prior antibiotic therapy, were stably subcultured. The sensitivity of culture was low when compared with that of PCR-based detection methods in valves of patients with endocarditis (44 and 81%, respectively), skin biopsy samples of patients with bacillary angiomatosis (43 and 100%, respectively), and lymph nodes of cat scratch disease (13 and 30%, respectively). Serological diagnosis was also more sensitive in cases of endocarditis (97%) and cat scratch disease (90%). Among endocarditis patients, the sensitivity of the shell vial culture assay was 28% when inoculated with blood samples and 44% when inoculated with valvular biopsy samples, and the sensitivity of both was significantly higher than that of culture on agar (5% for blood [P = 0.045] and 4% for valve biopsy samples [P < 0.0005]). The most efficient culture procedure was the subculture of blood culture broth into shell vials (sensitivity, 71%). For patients with endocarditis, previous antibiotic therapy significantly affected results of blood culture; no patient who had been administered antibiotics yielded a positive blood culture, whereas 80% of patients with no previous antibiotic therapy yielded positive blood cultures (P = 0.0006). Previous antibiotic therapy did not, however, prevent isolation ofBartonella sp. from cardiac valves but did prevent the establishment of strains, as none of the 15 isolates from treated patients could be successfully subcultured. For the diagnosis ofB. quintana bacteremia in homeless people, the efficiency of systematic subculture of blood culture broth onto agar was higher than that of direct blood plating (respective sensitivities, 98 and 10% [P < 10−7]). Nevertheless, both procedures are complementary, since when used together their sensitivity reached 100%. All homeless people with positive blood cultures had negative serology. The isolation rate of B. henselae from PCR-positive lymph nodes, in patients with cat scratch disease, was significantly lower than that from valves of endocarditis patients and skin biopsy samples from bacillary angiomatosis patients (13 and 33%, respectively [P = 0.084]). In cases of bacillary angiomatosis for which an agent was identified to species level, the isolation rate of B. henselae was lower than the isolation rate of B. quintana (28 and 64%, respectively [P = 0.003]). If culture is to be considered an efficient tool for the diagnosis of several Bartonella-related diseases, methodologies need to be improved, notably for the recovery of B. henselae from lymph nodes of patients with cat scratch disease.

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“…were isolated from 5 of the 14 patients whose blood samples were inoculated onto the appropriate media prior to antibiotic therapy and from the excised valves of two others. DNA specific for B. henselae or B. quintana was demonstrated in the blood or excised valves of an additional six patients by PCR amplification, followed by determination of amplicon nucleotide base sequence (15). Fiftyeight serum samples from 58 patients with antibody levels indicative of B. henselae CSD and with a local adenopathy associated with a cat scratch were also tested.…”

Section: Serum Selectionmentioning

confidence: 99%

Serological cross-reactions between Bartonella quintana, Bartonella henselae, and Coxiella burnetii

1996

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The clinical manifestations of Q fever and bartonelloses can be confused, especially in cases of infectious endocarditis. Differential diagnosis of the diseases is important because the treatments required for Q fever and bartonelloses are different. Laboratory confirmation of a suspected case of either Q fever or bartonelloses is most commonly made by antibody estimation with an indirect immunofluorescence assay. With an indirect immunofluorescence assay, 258 serum samples from patients with Q fever were tested against Bartonella henselae and Bartonella quintana antigens, and 77 serum samples from patients with infection by Bartonella sp. were tested against Coxiella burnetii antigen. Cross-reactivity was observed: more than 50% of the chronic Q fever patients tested had antibodies which reacted against B. henselae antigen to a significant level. This cross-reaction was confirmed by a cross-adsorption study and protein immunoblotting. However, because the levels of specific antibody titers in cases of Bartonella endocarditis are typically extremely high, low-level cross-reaction between C. burnetii antibodies and B. henselae antigen in cases of Q fever endocarditis should not lead to misdiagnosis, provided serology testing for both agents is performed.

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Identification of Bartonella (Rochalimaea) species among fastidious gram-negative bacteria on the basis of the partial sequence of the citrate-synthase gene

Cited by 63 publications

References 33 publications

Outcome and Treatment of Bartonella Endocarditis

Outcome and Treatment of Bartonella Endocarditis

Culture of Bartonella quintana and Bartonella henselae from Human Samples: a 5-Year Experience (1993 to 1998)

Serological cross-reactions between Bartonella quintana, Bartonella henselae, and Coxiella burnetii

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