Identification of bioactive metabolites from corn silk extracts by a combination of metabolite profiling, univariate statistical analysis and chemometrics

Hora, Natan R.S. da; Santana, Lucia Fonseca; Silva, Victor Diógenes A. da; Costa, Sílvia Lima; Zambotti-Villela, Leonardo; Colepicolo, Pio; Ferraz, Caline G.; Ribeiro, Paulo R.

doi:10.1016/j.foodchem.2021.130479

Cited by 19 publications

(8 citation statements)

References 34 publications

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“…They have shown various properties of interest such as antidiabetic [ 2 ], anticoagulant [ 3 ], antifungal [ 4 ] and antioxidant [ 5 , 6 ] activities, and as inhibitors of IgE antibodies by glycoproteins in the context of allergic diseases [ 7 ]. The composition of corn silk extract, which depends on the variety, determines its biological activity [ 8 , 9 ]. The polysaccharides contained in corn silk may have an antidepressant activity.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Corn Silk Extract Using HPLC/HRMS/MS Analyses and Bioinformatic Data Processing

Fougère

Zubrzycki

Elfakir

et al. 2023

Plants

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In addition to having different biological activities of interest, corn silks play a role in the defense of plants. While benzoxamines and flavonoids have already been identified as molecules of plant defense and growth mechanisms, knowledge on the phytochemical composition of corn silk is lacking. Such knowledge would make it possible to better select the most effective varieties to improve resistance or bioactive properties. In this article, an approach was implemented to map a corn silk extract in two complementary ways. The first one involved working with UHPLC/HRMS data and Kendrick and van Krevelen plots to highlight a homologous series of compounds, such as lipids from 17 to 23 carbons, monoglycosylated flavonoids from 21 to 24 carbons, diglycosylated flavonoids of 26 to 28 carbons and organic acids of 14 to 19 carbons. The second way was to analyze the sample in UHPLC/HRMS2 and to plot mass spectral similarity networks with the GNPS platform and Cytoscape software to refine identification. By combining the information obtained, we were able to propose an identification for 104 detected molecules, including 7 nitrogenous, 28 lipidic and 67 phenolic compounds, leading to the first detailed phytochemical analysis of corn silk extract.

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Section: Introductionmentioning

confidence: 99%

Characterization of Corn Silk Extract Using HPLC/HRMS/MS Analyses and Bioinformatic Data Processing

Fougère

Zubrzycki

Elfakir

et al. 2023

Plants

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“…MTT was added in each well at a final concentration of 2 mg/mL, and the cells were incubated for 2 h. After that, cells were lysed with 20% (w/v) sodium dodecyl sulfate (SDS) and 50% (v/v) dimethylformamide (DMF) solution (pH 4.7), in an overnight incubation at room temperature [54,55]. The absorbance was measured with a spectrophotometer microplate reader (570 nm), Thermo Scientific ® Flash Varioskan (Version 3001, Thermo Fisher Scientific, Vantaa, Finland).…”

Section: Cytotoxic Activity Assaymentioning

confidence: 99%

Structural Design, Synthesis and Antioxidant, Antileishmania, Anti-Inflammatory and Anticancer Activities of a Novel Quercetin Acetylated Derivative

et al. 2021

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Quercetin (Q) is a bioflavonoid with biological potential; however, poor solubility in water, extensive enzymatic metabolism and a reduced bioavailability limit its biopharmacological use. The aim of this study was to perform structural modification in Q by acetylation, thus, obtaining the quercetin pentaacetate (Q5) analogue, in order to investigate the biological potentials (antioxidant, antileishmania, anti-inflammatory and cytotoxicity activities) in cell cultures. Q5 was characterized by FTIR, 1H and 13C NMR spectra. The antioxidant potential was evaluated against the radical ABTS•+. The anti-inflammatory potential was evaluated by measuring the pro-inflammatory cytokine tumor necrosis factor (TNF) and the production of nitric oxide (NO) in peritoneal macrophages from BALB/c mice. Cytotoxicity tests were performed using the AlamarBlue method in cancer cells HepG2 (human hepatocarcinoma), HL-60 (promyelocytic leukemia) and MCR-5 (healthy human lung fibroblasts) as well as the MTT method for C6 cell cultures (rat glioma). Q and Q5 showed antioxidant activity of 29% and 18%, respectively, which is justified by the replacement of hydroxyls by acetyl groups. Q and Q5 showed concentration-dependent reductions in NO and TNF production (p < 0.05); Q and Q5 showed higher activity at concentrations > 40µM when compared to dexamethasone (20 µM). For the HL-60 lineage, Q5 demonstrated selectivity, inducing death in cancer cells, when compared to the healthy cell line MRC-5 (IC50 > 80 µM). Finally, the cytotoxic superiority of Q5 was verified (IC50 = 11 µM), which, at 50 µM for 24 h, induced changes in the morphology of C6 glioma cells characterized by a round body shape (not yet reported in the literature). The analogue Q5 had potential biological effects and may be promising for further investigations against other cell cultures, particularly neural ones.

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“…maydis is considered to be a precious food-based by-product of corn cultivation and is widely utilized in food and pharmaceutical industries. − S. maydis has received worldwide attention and can be utilized commercially as a nutritional and medicinal supplement to produce various value-added products such as corn silk tea, snacks, cosmetics, powder, medicines, and capsules. − On the basis of China National Intellectual Property Office, various types of healthy foods and pharmaceutical formulations, which were relevant to S. maydis, have been patented .…”

Section: Introductionmentioning

confidence: 99%

Utilization of the By-Product of Corn: Guided Identification of Bioactive Terpenoids from Stigma Maydis (Corn Silk)

Zhou

Niu

et al. 2023

J. Agric. Food Chem.

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Stigma maydis (corn silk) (S. maydis) is a food-based by-product of maize and possesses great nutritional and pharmaceutical value. This study aimed to explore bioactive components from S. maydis. By the guidance of bioactivity-guided approach and Global Natural Products Social (GNPS) molecular networking, 12 terpenoids were discovered from S. maydis. The structures of 11 undescribed compounds (1–11) were determined by detailed spectroscopic analyses, single-crystal X-ray diffraction analysis, specific rotation calculations, electronic circular dichroism (ECD) calculations, and NMR calculations. The neuroprotective and acetylcholinesterase (AChE) inhibitory effects of 1–12 were examined, and most of them showed significant or moderate activities. The underlying neuroprotective mechanism of 4 and 5 was revealed by Hoechst 33258, AO-EB, and JC-1 staining assays. This work illustrated the potential of S. maydis as a prospective natural source of bioactive compounds in food and pharmaceutical industries.

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Identification of bioactive metabolites from corn silk extracts by a combination of metabolite profiling, univariate statistical analysis and chemometrics

Cited by 19 publications

References 34 publications

Characterization of Corn Silk Extract Using HPLC/HRMS/MS Analyses and Bioinformatic Data Processing

Characterization of Corn Silk Extract Using HPLC/HRMS/MS Analyses and Bioinformatic Data Processing

Structural Design, Synthesis and Antioxidant, Antileishmania, Anti-Inflammatory and Anticancer Activities of a Novel Quercetin Acetylated Derivative

Utilization of the By-Product of Corn: Guided Identification of Bioactive Terpenoids from Stigma Maydis (Corn Silk)

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