Identification of biomarkers associated with partial epithelial to mesenchymal transition in the secretome of slug over-expressing hepatocellular carcinoma cells

Karaosmanoğlu, Oğuzhan; Banerjee, Sreeparna; Sivas, Hülya

doi:10.1007/s13402-018-0384-6

Cited by 48 publications

(40 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Complete EMT is observed in individuals with vital role of it in metastasis initiation. Karaosmanoğlu Oand their group demonstrated reduction of E-cadherin and upregulation of ZEB2 in complete EMT, and meanwhile partial EMT involves increase of E-cadherin and decrease of vimentin and ZEB2 [87]. It is worth notifying that the EMT might causean increasein the cancer stem-like cells (CSCs), which refers to the highly tumorigenic subpopulation of tumor cells that exists at the top of the hierarchical tumor cell society [88,89], leading to tumor heterogeneity and therapeutic resistance [89].…”

Section: Exosomes Participate In Liver Cancer Epithelial-mesenchymal mentioning

confidence: 99%

The significance of exosomes in the development and treatment of hepatocellular carcinoma

et al. 2020

View full text Add to dashboard Cite

Hepatocellular carcinoma (HCC) is the most commonmalignancy. Exsome plays a significant role in the elucidation of signal transduction pathways between hepatoma cells, angiogenesis and early diagnosis of HCC. Exosomes are small vesicular structures that mediate interaction between different types of cells, and contain a variety of components (including DNA, RNA, and proteins). Numerous studies have shown that these substances in exosomes are involved in growth, metastasis and angiogenesis in liver cancer, and then inhibited the growth of liver cancer by blocking the signaling pathway of liver cancer cells. In addition, the exosomal substances could also be used as markers for screening early liver cancer. In this review, we summarized to reveal the significance of exosomes in the occurrence, development, diagnosis and treatment of HCC, which in turn might help us to further elucidate the mechanism of exosomes in HCC, and promote the use of exosomes in the clinical diagnosis and treatment of HCC.

show abstract

Section: Exosomes Participate In Liver Cancer Epithelial-mesenchymal mentioning

confidence: 99%

The significance of exosomes in the development and treatment of hepatocellular carcinoma

et al. 2020

View full text Add to dashboard Cite

show abstract

“…HCC represents > 90% of primary liver cancers and is a major health problem due to its poor prognosis [8,20]. A number of risk factors for the development of HCC has been identified, and over 50% of HCC cases are attributable to persistent HBV The data shown are from one of three independent experiments which yielded similar results.…”

Section: Discussionmentioning

confidence: 77%

“…However, those biomarkers display limited sensitivity and specificity, especially with respect to early HCC stages and, therefore, combinations with other newly-identified candidate biomarkers are currently being evaluated [6]. Over the past years, technologies including whole genome sequencing (WGS), RNA-sequencing (RNA-seq) and proteomic profiling have led to a new era in biomarker development that has improved our understanding of complex interactions between proteins, genes and noncoding RNAs in hepatocarcinogenesis in different settings [7][8][9]. Most studies did, however, not distinguish HBV-related HCCs from HCCs resulting from other factors, and the analyzed tumor tissues were rarely matched with adjacent peritumor tissues (APTs) from the same patients.…”

Section: Introductionmentioning

confidence: 99%

Multiple novel hepatocellular carcinoma signature genes are commonly controlled by the master pluripotency factor OCT4

Zhang

Chen

et al. 2019

Cell Oncol.

View full text Add to dashboard Cite

Background Worldwide, hepatocellular carcinoma (HCC) is a common solid tumor with a poor prognosis. HCC is often due to hepatitis B virus (HBV) infection. As yet, efficacious HCC treatment regimens for late-stage HCC patients are lacking. Therefore, the identification of more specific and sensitive biomarkers for its early diagnosis and treatment remains an urgent need. Methods Total RNAs from paired HBV-derived HCC tumors and adjacent peritumor tissues (APTs) were subjected to RNA sequencing (RNA-seq), and differentially expressed genes (DEGs) between HCC tumors and APTs were selected and verified. Results We identified 166 DEGs and found that eight top-ranked and verified DEGs (TK1, CTTN, CEP72, TRIP13, FTH1, FLAD1, CHRM2, AMBP) all contained putative OCT4 binding motifs in their promoter regions. TK1, TRIP13 and OCT4 were found to exhibit concurrent higher expression levels in HCC tumors than in APTs. The mRNA levels of TK1, TRIP13 and OCT4 in a cohort of 384 HCC samples from the TCGA database were all found to be negatively correlated with patient overall survival, relapse-free survival and progression-free survival, underscoring the HCC biomarker status of TK1 and TRIP13 on one hand, and implicating their association with OCT4 on the other hand. Furthermore, OCT4 proteins were found to bind to the promoters of both genes in vitro and in vivo. Knocking out OCT4 in HCC-derived cell lines reduced the expression of TK1 and TRIP13 and significantly decreased their tumorigenicity. Chao Ye, Xiaoqian Zhang and Xinyu Chen contributed equally to this work.

show abstract

“…Neutral red uptake assay was performed to determine the viability of cells as previously described . Briefly, cells were seeded at a density of 1 × 10 4 cm −2 .…”

Section: Methodsmentioning

confidence: 99%

ZnO microparticle‐loaded chitosan/poly(vinyl alcohol)/acacia gum nanosphere‐based nanocomposite thin film wound dressings for accelerated wound healing

Güldiken

Karaosmanoğlu

Sivas

et al. 2019

J of Applied Polymer Sci

Self Cite

View full text Add to dashboard Cite

The present study deals with the development of novel ZnO microparticle‐loaded chitosan/poly(vinyl alcohol)/acacia gum nanosphere‐based nanocomposite thin films through electrospraying and evaluation of their potential use in wound healing applications for skin. ZnO microparticles were synthesized and used as bioactive agents. Morphology, size distribution, structure, and dispersion of the synthesized ZnO microparticles were analyzed by scanning electron microscopy (SEM), energy‐dispersive X‐ray spectroscopy, Fourier transform infrared spectroscopy, and transmission electron microscopy (TEM). ZnO microparticles were incorporated into the ternary nanocomposite films by electrospraying technique. Thermogravimetric analyses reveal that incorporation of ZnO microparticles into the nanocomposite structure improves the thermal stability. Mechanical analyses show that tensile strength reaches to the maximum value of 12.75 MPa with 0.6 wt % ZnO content. SEM and TEM micrographs demonstrate that the nanocomposite films consist of nanospheres with nanocapsular structures whose sizes are mostly between 250 and 550 nm. Viability tests established prevailing cellular performance of the fibroblasts on 0.6 wt % ZnO microparticle‐loaded nanocomposite films with a viability percentage of 160% compared to the control group. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020, 137, 48445.

show abstract

Identification of biomarkers associated with partial epithelial to mesenchymal transition in the secretome of slug over-expressing hepatocellular carcinoma cells

Abstract: From our data we conclude that the exosomal proteins identified may be considered as potential non-invasive biomarkers for chemo-resistance and partial EMT in HCC.

Cited by 48 publications

References 70 publications

The significance of exosomes in the development and treatment of hepatocellular carcinoma

The significance of exosomes in the development and treatment of hepatocellular carcinoma

Multiple novel hepatocellular carcinoma signature genes are commonly controlled by the master pluripotency factor OCT4

ZnO microparticle‐loaded chitosan/poly(vinyl alcohol)/acacia gum nanosphere‐based nanocomposite thin film wound dressings for accelerated wound healing

Contact Info

Product

Resources

About