Identification of Bovine Mastitis Associated Pathogens by Multiplex PCR

Sk, Kashyap

doi:10.19080/jdvs.2017.03.555622

Cited by 6 publications

(5 citation statements)

References 12 publications

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“…Many studies rely solely on culture techniques and biochemical tests [8,32,36,65]. Our research and other publications [66][67][68][69] confirm the necessity of complementing these methods with molecular techniques such as PCR or real-time PCR reactions. However, it should be considered that the higher sensibility of PCR can be related to the DNA amplification of a not significant number of bacterial colonies in milk (that cannot be considered as responsible for mastitis), or dead bacterial cells.…”

Section: Discussionsupporting

confidence: 73%

“…The crucial preliminary step before amplification is the extraction of bacterial DNA. In certain publications the standard isolation procedures [66,67,[69][70][71] are described as involving direct extraction from milk samples by use of commercial kits. In our research, as well as employing the standard approach, we also evaluated the effect of an additional 18-hour pre-incubation of milk samples in TSB on the sensitivity of the detection of micro-organisms in the PCR reaction.…”

Section: Discussionmentioning

confidence: 99%

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Molecular and phenotypic identification of bacterial species isolated from cows with mastitis from three regions of Poland

Dobrut,

Siemińska,

Sroka-Oleksiak

et al. 2024

BMC Vet Res

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Background Bovine mastitis is a widespread disease affecting dairy cattle worldwide and it generates substantial losses for dairy farmers. Mastitis may be caused by bacteria, fungi or algae. The most common species isolated from infected milk are, among others, Streptococcus spp., Escherichia coli, Staphylococcus aureus and non-aureus staphylococci and mammaliicocci. The aim of this paper is to determine the frequency of occurrence of bacterial species in milk samples from cows with mastitis from three regions of Poland: the north-east, the south-west and the south. To this end 203 milk samples taken from cows with a clinical form (CM) of mastitis (n = 100) and healthy animals (n = 103) were examined, which included culture on an appropriate medium followed by molecular detection of E. coli, S. aureus, Streptococcus agalactiae and Streptococcus uberis, as one of the most common species isolated from mastitis milk. Results The results obtained indicated that S. uberis was the most commonly cultivated CM species (38%, n = 38), followed by S. aureus (22%, n = 22), E. coli (21%, n = 21) and S. agalactiae (18%, n = 18). Similar frequencies in molecular methods were obtained for S. uberis (35.1%) and S. aureus (28.0%). The variation of sensitivity of both methods may be responsible for the differences in the E. coli (41.0%, p = 0.002) and S. agalactiae (5.0%, p = 0.004) detection rates. Significant differences in composition of species between three regions of Poland were noted for E. coli incidence (p < 0.001), in both the culture and molecular methods, but data obtained by the PCR method indicated that this species was the least common in north-eastern Poland, while the culture method showed that in north-eastern Poland E. coli was the most common species. Significant differences for the molecular method were also observed for S. uberis (p < 0.001) and S. aureus (p < 0.001). Both species were most common in southern and south-western Poland. Conclusions The results obtained confirm the need to introduce rapid molecular tests for veterinary diagnostics, as well as providing important epidemiological data, to the best of our knowledge data on Polish cows in selected areas of Poland is lacking.

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Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Molecular and phenotypic identification of bacterial species isolated from cows with mastitis from three regions of Poland

Dobrut,

Siemińska,

Sroka-Oleksiak

et al. 2024

BMC Vet Res

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“…In non mastitc group, Respective values are reveled 10.89±0.23, 33.4 ±0.84, 7.1±0.12, 6.84±0.41, 50.96 ± 0.59, 60.2±0.86, 3.2±0. 28. The current study reveled significant decrease in PCV, RBCc and Hb with increase ratio in WBCc in infected cows compared to control cows.…”

Section: Resultssupporting

confidence: 63%

“…Among different bacterial pathogens isolated, The current results showed that E. coli at (33.8%), Its agreement pattern was noticed in Iraq by reference [26], which is 36 % as predominant coliform pathogen than other species from milk contamination .further the our result high relatively show than by [27]which is 30% in Egypt. on the other hand, k.pneumonia constituted 11.7 % are the second prevalent bacteria finally Proteus mirabilis investigate as a low presented 10.2% from total isolate similar to [28,29]. However over all prevalence of these pathogens in this study is contrast to the other authors results were reported low situation of K.pneumonia and proteus mirabilis respectively [30,31] bad hygiene due to increases the prevalence of risk infected to intra mammary with coliform pathogens [31].…”

Section: Identification and Isolationmentioning

confidence: 45%

Evaluation Effects of Coliform Mastitis on Hematological and Biochemical Parameters in Dairy Cows, in Kirkuk City, Iraq

Al-Bayati

2023

IOP Conf. Ser.: Earth Environ. Sci.

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This study conducted to investigate sub clinical mastitis caused by some coliform pathogens as a common causes mastitis in the cows and to detect some hematological & biochemical parameters, for this purpose 100 milk samples and100 blood samples were collected from apparently cows brought to the veterinary hospital & from different heifer’s in Kirkuk governate, Iraq. our results showed that the incidence of coliform mastitis by using different diagnostic methods. the incidence mastitis to CMT according of management & hygiene milk of processing recorded 35 (42.6 %), 47 ( 57.3%) respectively., However the bacteriologically examination depending on isolation, biochemical manually and vitek system identification. revealed, the incidence as 82.9% positive for bacterial identification reveled that E.coli was predominant ( 33.8 %) bacteria Followed by K. pneumonia and. Proteus mirabilis (11.7 %) &(10.2)% respectively. further hematological parameters we noted that showed anemia with significant decrease (p<0.05) in Hemoglobin, Total red blood cell count, Paced cell volume, lymphocyte and slightly decrease in, while record increase in granulocyte and Wight blood cell count. On the other hand the biochemical profile we showed significant increase in ALT, AST enzymes, While decrease significant(p<0.05) in ALP, Ca, Cl, P, Mg & zn. We conclude from this study, The prevalence of pathogenic bacteria in the milk resulted in a significant alteration in the hamato-biochemical profile in the current study reveled significant monitors for Bovine mastitis and estimate the efficiency and quality of milk consumption.

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“…Bovine mastitis (BM) is an inflammatory disease of dairy cows caused by various opportunistic bacteria that reduce milk yield and deteriorate the quality of milk composition [37,38]. BM is a threat to dairy farms and farmers [39]. The findings of the current study showed that the prevalence of S. aureus in mastitis milk samples was 100% (n = 48).…”

Section: Discussionmentioning

confidence: 74%

Molecular Detection of Multidrug Resistant Staphylococcus aureus Isolated from Bovine Mastitis Milk in Bangladesh

Salauddin

Akter

Hossain

et al. 2020

Veterinary Sciences

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The current study was conducted to isolate and identify multidrug-resistant Staphylococcus aureus (MDR-SA) from mastitis milk samples and to determine their antimicrobial susceptibility pattern. A total of 48 bovine mastitis (BM) milk samples were collected from different parts of the Rangpur division, Bangladesh. After the collection of milk samples, mastitis was confirmed using the California mastitis test. Isolation and identification of Staphylococcus aureus were performed using conventional cultural and biochemical tests as well as using molecular methods of PCR. Nucleotide sequence analysis of the 23S rRNA gene of Staphylococcus aureus was determined. The antibiogram of the isolated bacteria was conducted using the disc diffusion method. Phylogenetic analysis of 23S rRNA was done using MEGA 7, ClustalW multiple sequence alignment, and NCBI-BLAST tools, where the sequence of the isolate showed 98% to 99% identity. Antibiogram test using 15 antimicrobial agents showed that all of the Staphylococcus aureus isolates were classified as multidrug-resistant (MDR). It was found that the isolates were resistant to tetracycline, novobiocin, methicillin, vancomycin, and cephradine, and the isolates were sensitive to ciprofloxacin, azithromycin, norfloxacin, levofloxacin, gentamicin, and amoxicillin. The detection of MDR-SA in mastitis milk is alarming and represents a great public health concern. The findings of the present study help identify Staphylococcus aureus at the molecular level using 23S rRNA gene sequencing and will help select the appropriate and effective antimicrobial agent to control BM in the northern part of Bangladesh.

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Identification of Bovine Mastitis Associated Pathogens by Multiplex PCR

Abstract: Isolation and identification of bacteriaCulture characteristics: The milk samples were subjected to aerobic cultivation. Each milk sample was streaked on Nutrient

Cited by 6 publications

References 12 publications

Molecular and phenotypic identification of bacterial species isolated from cows with mastitis from three regions of Poland

Molecular and phenotypic identification of bacterial species isolated from cows with mastitis from three regions of Poland

Evaluation Effects of Coliform Mastitis on Hematological and Biochemical Parameters in Dairy Cows, in Kirkuk City, Iraq

Molecular Detection of Multidrug Resistant Staphylococcus aureus Isolated from Bovine Mastitis Milk in Bangladesh

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