Identification of Candida species in vaginal flora using conventional and molecular methods

Ameen, Fuad; Moslem, Mohamed A.; Tami, Mona Al; AlAjlan, Hisham; AlQahtani, Nasser Ayidh

doi:10.1016/j.mycmed.2017.04.105

Cited by 21 publications

(9 citation statements)

References 18 publications

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“…It is an opportunistic pathogen causing VVC. However, C. albicans has been reported to be the dominant fungal species in asymptomatic non-pregnant women and it colonizes 20% of women (Drell et al, 2013;Ameen et al, 2017;Chee et al, 2020). Our recent study supported the previous findings that C. albicans and/or other Candida spp.…”

Section: Bacteria and Fungisupporting

confidence: 90%

Healthy Vaginal Microbiota and Influence of Probiotics Across the Female Life Span

Lehtoranta¹,

Ala-Jaakkola²,

Laitila³

et al. 2022

Front. Microbiol.

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Vaginal microbiota plays a central role in women’s health and reproduction. Vaginal microbiota is dynamic and shaped by hormonal shifts in each stage of a woman’s life from pre-puberty to postmenopause. Current research has mainly focused on vaginal bacterial and fungal members of the community and emphasized their role in disease. However, the impact of balanced vaginal microbiota on health and its interaction with the host is yet poorly understood. High abundance of vaginal lactobacilli is most strongly associated with health, but the concept of health may vary as vaginal dysbiosis may be asymptomatic. Furthermore, there is a lot of variation between ethnic groups in terms of dominating vaginal bacteria. Probiotic lactobacilli could be a safe and natural means to balance and maintain healthy vaginal microbiota. Research evidence is accumulating on their role in supporting women’s health throughout life. This review describes the current literature on vaginal microbiota, the major factors affecting its composition, and how the communities change in different life stages. Furthermore, we focused on reviewing available literature on probiotics and their impact on vaginal microbiota and health.

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Section: Bacteria and Fungisupporting

confidence: 90%

Healthy Vaginal Microbiota and Influence of Probiotics Across the Female Life Span

Lehtoranta¹,

Ala-Jaakkola²,

Laitila³

et al. 2022

Front. Microbiol.

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“…Regarding the age of the patients, the majority of the patients were between [18-40 years old]. This is similar to the results of several studies carried out on vaginal candidiasis where the age group most affected by vaginal candidiasis was that of women in the period of genital activity [7][8][9][10][11][12]14,16,17,26]. However, the majority of these studies did not specifically focus on Candida non albicans candidiasis.…”

Section: Discussionsupporting

confidence: 69%

“…It is the most common genital infection in women and affects nearly 75% of sexually active women [4][5][6][7]. While Candida albicans has long been implicated in the occurrence of vaginal candidiasis, in recent years we have seen an emergence of Candida non albicans in women of childbearing age [4,8,9]. Among these species, Candida glabrata was the most frequent [2,10,11].…”

Section: Introductionmentioning

confidence: 99%

Vaginal Candidiasis Caused by Candida Non Albicans: Epidemiological and Mycological Aspects at the University Hospital Center of Brazzaville

G¹,

Br²,

NSB³

2022

J Inter Med Res & Rep

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Introduction: Vaginal candidiasis is a mycosis caused by a yeast of the genus Candida. Candida albicans was for a long time the first etiology of these mycoses. However, in recent years, an increasing involvement of non-albicans Candida species has been observed. Objective: To describe the epidemiological and mycological aspects of vaginal candidiasis due to Candida non albicans in women who have performed an analysis of the vaginal sample in the Parasitology Mycology laboratory of the University Hospital Center of Brazzaville. Patients, Materials And Method: This was a descriptive and analytical cross-sectional study from July to October 2019 (i.e. 3 months). It concerned all the patients received at the Parasitology-mycology and parasitic immunology laboratory of the CHUB for an analysis of the vaginal sample. The diagnosis was made after direct examination and culture of samples on Sabouraud Chloramphenicol medium with and without Actidione*. The filamentation test on calf serum and the study of the biochemical characteristics of the yeast colonies using the API candida strips from Biomérieux were carried out for each positive culture. Statistical analysis was performed using Epi-info 7.2.2.6 softwares. Results: The frequency of Candida non albicans yeast infection was 64.7%. The median age of patients with Candida non albicans vaginal candidiasis was 35.5 years (25.0 years - 47.0 years) with extremes of 18 and 59 years old. The age group between 18 and 40 was the most represented (68.2%). These were women with secondary education (n=12 ; 54.5%), with a notion of a single sexual partner (n=17 ; 77.3%), pregnant (n=6 ; 27, 3%). In 22.7% there was poor faecal hygiene. The main reasons for taking the samples were… and the non-albicans Candida species were represented by Candida glabrata (36.4%), Candida tropicalis (18.2%), Candida krusei (13.6%), Candida famata (13.6%), Candida parapsilosis (9.1%) and Candida spp (4.6%). Analytical Study Conclusion: Candida non albicansremains the common fungus in our context with a predominance of Candida glabrata. Age, poor faecal hygiene could have a link with the occurrence of these fungal infections, hence the importance of good awareness and systematic research in young women.

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“…The majority of the cervicovaginal infections and vaginal discomfort, are due to either bacteria such as Gardenella vaginalis or Atopobium ( Liu M.B. et al, 2013 ) or by fungi such as Candida albicans ( Ameen et al, 2017 ). The cervical epithelium is therefore vulnerable under the influence of infectious microbiome agents, including viruses such as HPV ( Widschwendter et al, 2003 ; Smith et al, 2012 ; Piyathilake et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Cervicovaginal Fungi and Bacteria Associated With Cervical Intraepithelial Neoplasia and High-Risk Human Papillomavirus Infections in a Hispanic Population

et al. 2018

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The human cervicovaginal microbiota resides at an interface between the host and the environment and may affect susceptibility to disease. Puerto Rican women have high human papillomavirus (HPV) infection and cervical cancer rates. We hypothesized that the population structure of the cervicovaginal bacterial and fungal biota changed with cervical squamous intraepithelial lesions and HPV infections. DNA was extracted from cervix, introitus, and anal sites of 62 patients attending high-risk San Juan clinics. The 16S rRNA V4 region and ITS-2 fungal regions were amplified and sequenced using Illumina technology. HPV genotyping was determined by reverse hybridization with the HPV SPF10-LiPA25 kit. HPV prevalence was 84% of which ∼44% subjects were infected with high-risk HPV, ∼35% were co-infected with as many as 9 HPV types and ∼5% were infected with exclusively low-risk HPV types. HPV diversity did not change with cervical dysplasia. Cervical bacteria were more diverse in patients with CIN3 pre-cancerous lesions. We found enrichment of Atopobium vaginae and Gardnerella vaginalis in patients with CIN3 lesions. We found no significant bacterial biomarkers associated with HPV infections. Fungal diversity was significantly higher in cervical samples with high-risk HPV and introitus samples of patients with Atypical Squamous Cells of Undetermined Significance (ASCUS). Fungal biomarker signatures for vagina and cervix include Sporidiobolaceae and Sacharomyces for ASCUS, and Malassezia for high-risk HPV infections. Our combined data suggests that specific cervicovaginal bacterial and fungal populations are related to the host epithelial microenvironment, and could play roles in cervical dysplasia.

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Identification of Candida species in vaginal flora using conventional and molecular methods

Cited by 21 publications

References 18 publications

Healthy Vaginal Microbiota and Influence of Probiotics Across the Female Life Span

Healthy Vaginal Microbiota and Influence of Probiotics Across the Female Life Span

Vaginal Candidiasis Caused by Candida Non Albicans: Epidemiological and Mycological Aspects at the University Hospital Center of Brazzaville

Cervicovaginal Fungi and Bacteria Associated With Cervical Intraepithelial Neoplasia and High-Risk Human Papillomavirus Infections in a Hispanic Population

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