Identification of Candida Species Using MP65 Gene and Evaluation of the Candida albicans MP65 Gene Expression in BALB/C Mice

Bineshian, Farahnaz; Yadegari, Mohammad Hossein; Sharifi, Zohreh; Eidgahi, Mohammadreza Akbari; Nasr, Reza

doi:10.5812/jjm.8(5)2015.18984

Cited by 10 publications

(9 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…albicans complex spp. are the most frequently isolated from clinical samples [33,41]. However, cryptic species within this complex such as C. dubliniensis and C. africana, are routinely misidentified or unidentified.…”

Section: Discussionmentioning

confidence: 99%

Identification of Cryptic Species of Four Candida Complexes in a Culture Collection

Fontecha¹,

Montes²,

Ortiz³

et al. 2019

Preprint

View full text Add to dashboard Cite

Candida spp. are the most common cause of fungal infections worldwide. The taxonomy of Candida is controversial and has undergone recent changes due to novel genetically related species. Therefore, some complexes of cryptic species have been proposed. In clinical settings, the correct identification of Candida species is relevant since some species are associated with high resistance to antifungal drugs and increased virulence. This study aimed to identify the species of four Candida complexes (C. albicans, C. glabrata, C. parapsilosis, and C. haemulonii) by molecular methods. This is the first report of six cryptic Candida species in Honduras: C. dubliniensis, C. africana, C. duobushaemulonii, C. orthopsilosis, and C. metapsilosis, and it is also the first report of the allele hwp1-2 of C. albicans sensu stricto. It was not possible to demonstrate the existence of C. auris among the isolates of the C. haemulonii complex. We also propose a simple method based on PCR-RFLP for the discrimination of the multi-resistant pathogen C. auris within the C. haemulonii complex.

show abstract

Section: Discussionmentioning

confidence: 99%

Identification of Cryptic Species of Four Candida Complexes in a Culture Collection

Fontecha¹,

Montes²,

Ortiz³

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…The mean mRNA levels for each gene were calculated from at least three independent biological replicates. The expression levels of target genes were analyzed according to the 2 -∆∆Ct method (28). Differences between resistant and susceptible groups were analyzed with independent sample t-test.…”

Section: Quantitative Real-time Pcrmentioning

confidence: 99%

Gene Expression Analysis of Key Players Associated with Fluconazole Resistance in Candida albicans

Zhang

Xu²,

et al. 2019

Jundishapur J Microbiol

View full text Add to dashboard Cite

Background: Fluconazole resistance in Candida albicans has become a serious public health problem. Most previous studies have focused on deciphering the relationship between fluconazole resistance and amino acid substitutions in ERG11, which encodes cytochrome P450 lanosterol 14α-demethylase whose enzymatic activity is inhibited by azoles. However, azole resistance in C. albicans is a multifactorial phenomenon and several lines of evidence indicate that other genes and mechanisms may contribute to the development of fluconazole resistance. Objectives: The present study aimed to investigate the underlying role of six genes in fluconazole-resistant clinical strains of C. albicans, including ERG11, RTA2, and the efflux pump genes CDR1, CDR2, MDR1, and FLU1. Methods: We collected 40 fluconazole-resistant isolates and 40 susceptible isolates from patients with Candida infections in the First Affiliated Hospital of Nanchang University in China from 2005 to 2008. The susceptibility of the isolates to antifungal agents was tested by the M27-A3 broth microdilution method following the Clinical and Laboratory Standards Institute (CLSI) guidelines. Then, the gene expression levels of several key players in azole resistance were quantified. Results: Most fluconazole-resistant strains analyzed in this study were found to be cross-resistant to ketoconazole, itraconazole, and clotrimazole. We observed that the FLU1 gene expression significantly increased (P < 0.05) and exhibited major changes in most of the fluconazole-resistant isolates (75.0%). In addition, the expression of a novel gene, RTA2, was remarkably upregulated (P < 0.05). Interestingly, we found that 10% of the fluconazole-resistant isolates were simultaneously associated with ERG11 mutation and overexpression of RTA2, CDR1, and FLU1 genes. Unlike other studies, we did not find any difference in the expression of CDR2, MDR1, and ERG11 genes between the fluconazole-susceptible and resistant isolates. Conclusions: Our findings suggested that the overexpression of FLU1 and RTA2 genes may cause azole resistance; this finding had not been reported previously in clinical isolates of C. albicans. The upregulation of FLU1 and RTA2 genes was the predominant mechanism of fluconazole resistance in C. albicans in China.

show abstract

“…Candidiasis is a widespread fungal infection caused by various Candida species, especially in acquired immune deficiency syndrome (AIDS) patients, immunocompromised individuals, patients with hematopoietic stem cells or organ transplantations, and those who present underlying valvular heart diseases or have received long-term antibiotic therapy (Bineshian et al, 2015;Rezazadeh et al, 2016;Ortiz et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Probe-based qPCR Assay for Rapid Detection of Predominant Candida glabrata Sequence Type in Korea

Bae

Lee

Jin

2019

BSL

View full text Add to dashboard Cite

Recent years have seen an increase in the incidence of candidiasis caused by non-albicans Candida (NAC) species. In fact, C. glabrata is now second only to C. albicans as the most common cause of invasive candidiasis. Therefore, the rapid genotyping specifically for C. glabrata is required for early diagnosis and treatment of candidiasis. A number of genotyping assays have been developed to differentiate C. glabrata sequence types (STs), but they have several limitations. In the previous study, multi-locus sequence typing (MLST) has performed with a total of 101 C. glabrata clinical isolates to analyze the prevalent C. glabrata STs in Korea. A total of 11 different C. glabrata STs were identified and, among them, ST-138 was the most commonly classified. Thus, a novel probe-based quantitative PCR (qPCR) assay was developed and evaluated for rapid and accurate identification of the predominant C. glabrata ST-138 in Korea. Two primer pairs and hybridization probe sets were designed for the amplification of internal transcribed spacer 1 (ITS1) region and TRP1 gene. Analytical sensitivity of the probe-based qPCR assay was 100 ng to 10 pg and 100 ng to 100 pg (per 1 μL), which target ITS1 region and TRP1 gene, respectively. This assay did not react with any other Candida species and bacteria except C. glabrata. Of the 101 clinical isolates, 99 cases (98%) were concordant with MLST results. This novel probebased qPCR assay proved to be rapid, sensitive, highly specific, reproducible, and cost-effective than other genotyping assay for C. glabrata ST-138 identification.

show abstract

Identification of Candida Species Using MP65 Gene and Evaluation of the Candida albicans MP65 Gene Expression in BALB/C Mice

Cited by 10 publications

References 28 publications

Identification of Cryptic Species of Four Candida Complexes in a Culture Collection

Identification of Cryptic Species of Four Candida Complexes in a Culture Collection

Gene Expression Analysis of Key Players Associated with Fluconazole Resistance in Candida albicans

Probe-based qPCR Assay for Rapid Detection of Predominant Candida glabrata Sequence Type in Korea

Contact Info

Product

Resources

About