Identification of candidate chemosensory genes in the antennal transcriptome of Tenebrio molitor (Coleoptera: Tenebrionidae)

Liu, Su; Rao, Xiang‐Jun; Li, Mao-Ye; Feng, Ming-Feng; He, Mengzhu; Li, Shiguang

doi:10.1016/j.cbd.2015.01.004

Cited by 49 publications

(51 citation statements)

References 58 publications

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“…Tenebrio molitor (Liu et al, 2015). These studies suggest that OBPs may have other non-olfactory functions.…”

Section: Discussionmentioning

confidence: 77%

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Antennal transcriptome and odorant binding protein expression profiles of an invasive mealybug and its parasitoid

Nie

et al. 2017

J Applied Entomology

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Olfaction is fundamental to insect survival due to its roles in host location and interspecific communications. Here, we analysed the transcriptomes of odorant binding proteins (OBPs) from the invasive mealybug Phenacoccus solenopsis and its endopara- AbamOBP30 were expressed at high levels in male antenna. This study for the first time identified the chemosensory genes and determined their expression patterns in different tissues of the two species. Our results provide important molecular information for the exploration of chemosensory mechanisms in these two species.

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“…Tenebrio molitor (Liu et al, 2015). These studies suggest that OBPs may have other non-olfactory functions.…”

Section: Discussionmentioning

confidence: 77%

“…High expression of OBPs in non‐olfactory tissues was also reported in other insect species including Sogatella furcifera (He & He, ), Sclerodermus sp. (Zhou, Min, et al., ) and Tenebrio molitor (Liu et al., ). These studies suggest that OBPs may have other non‐olfactory functions.…”

Section: Discussionmentioning

confidence: 99%

Antennal transcriptome and odorant binding protein expression profiles of an invasive mealybug and its parasitoid

Nie

et al. 2017

J Applied Entomology

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“…First, tBLASTn algorithm with E-value < 10 −5 was used to identify homologs of B. longissima unigenes using as queries published chemosensory gene sequences (ORs, IRs, SNMPs, OBPs and CSPs) from other coleopteran species ( T. castaneum 28, 37, 48 , Tomicus yunnanensis 54 , Megacyllene caryae 30 , Ips typographus and Dendroctonus ponderosae 29 , Agrilus planipennis 49 , Batocera horsfieldi 55 , Anomala corpulenta 44, 47 , Colaphellus bowringi 31 , Tenebrio molitor 46 , Dendroctonus valens 45 , Anoplophora glabripennis 43 , Phyllotreta striolata 42 , Ambrostoma quadriimpressum ) 41 . Then, the resulting candidates were used as queries further to identify additional genes (tBLASTx and BLASTp).…”

Section: Methodsmentioning

confidence: 99%

Antennal and abdominal transcriptomes reveal chemosensory gene families in the coconut hispine beetle, Brontispa longissima

Bin¹,

Qu²,

Li³

et al. 2017

Sci Rep

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Antennal and abdominal transcriptomes of males and females of the coconut hispine beetle Brontispa longissima were sequenced to identify and compare the expression patterns of genes involved in odorant reception and detection. Representative proteins from the chemosensory gene families likely essential for insect olfaction were identified. These include 48 odorant receptors (ORs), 19 ionotropic receptors (IRs), 4 sensory neuron membrane proteins (SNMPs), 34 odorant binding proteins (OBPs) and 16 chemosensory proteins (CSPs). Phylogenetic analysis revealed the evolutionary relationship of these proteins with homologs from Coleopterans or other insects, and led to the identification of putative aggregation pheromone receptors in B. longissima. Comparative expression analysis performed by calculating FPKM values were also validated using quantitative real time-PCR (qPCR). The results revealed that all ORs and antennal IRs, two IR co-receptors (BlonIR8a and BlonIR25a) and one SNMP (BlonSNMP1a) were predominantly expressed in antennae when compared to abdomens, and approximately half of the OBPs (19) and CSPs (7) were enriched in antennae. These findings for the first time reveal the identification of key molecular components in B. longissima olfaction and provide a valuable resource for future functional analyses of olfaction, and identification of potential targets to control this quarantine pest.

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“…During olfactory perception, the odorous molecules are collected by various sensors and converted into electrical signals for complex recognition. Generally, proteins associated with the olfactory reception include odorant‐binding protein (OBP), chemosensory protein, odorant receptor, ionotropic receptor, sensory neuron membrane protein, and so on (S. Liu et al, ).…”

Section: Introductionmentioning

confidence: 99%

Antennal transcriptome analysis of the maize weevil Sitophilus zeamais: Identification and tissue expression profiling of candidate odorant‐binding protein genes

Tang

Shen

Zhang

et al. 2019

Arch Insect Biochem Physiol

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Our bioassays reviewed that antennae played crucial roles in the responses of maize weevil (Sitophilus zeamais) to food and sex volatiles. In order to identify the maize weevil odorant-binding protein (OBP) genes, we analyzed its antennal transcriptome. In total, 21,587,928 high-quality clean reads were obtained from RNA-seq, 52,206 unigenes were assembled, and 25,744 unigenes showed significant similarity (E value < 10 −5 ) to known proteins in the NCBI nonredundant protein database. From those unigenes, we identified 41 candidate OBP proteins, which could be categorized into dimeric OBPs subfamily, minus-C OBPs subfamily, and classical OBPs subfamily. Phylogenic analysis indicated that most maize weevil OBPs were closely related to their orthologues in other beetles of the Superfamily Curculionoidea. We further investigated the expression profiles of those candidate OBP genes by quantitative real-time polymerase chain reaction. Twenty-six of forty-one maize weevil OBP genes were highly expressed in the antennae or other parts of the head. The rest were expressed in the legs, wings, or other tested tissues. The antennal transcriptomic data and candidate OBP genes Arch. Insect Biochem. Physiol. 2019;101:e21542.wileyonlinelibrary.com/journal/arch

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Identification of candidate chemosensory genes in the antennal transcriptome of Tenebrio molitor (Coleoptera: Tenebrionidae)

Cited by 49 publications

References 58 publications

Antennal transcriptome and odorant binding protein expression profiles of an invasive mealybug and its parasitoid

Antennal transcriptome and odorant binding protein expression profiles of an invasive mealybug and its parasitoid

Antennal and abdominal transcriptomes reveal chemosensory gene families in the coconut hispine beetle, Brontispa longissima

Antennal transcriptome analysis of the maize weevil Sitophilus zeamais: Identification and tissue expression profiling of candidate odorant‐binding protein genes

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