Identification of carotenoids in ovarian tissue in women

Czeczuga-Semeniuk, Ewa; Wołczyński, Sławomir

doi:10.3892/or.14.5.1385

Cited by 13 publications

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“…According to literature, β-carotene and lycopene are the most abundant carotenoids in the human tissues [21,22]. However, we did not confirm the presence of lycopene in the studied ovarian tissues [17]. This may result from the fact that lycopene acts as an intermediate link in the formation of carotenoids, such as β-carotene, or its role as a free radical scavenger [23].…”

Section: Discussioncontrasting

confidence: 55%

“…The presence of carotenoid pigments in physiological and pathological ovarian tissues has been previously demonstrated by our team [17,18]. Furthermore, among the ovarian epithelial neoplasms, the endometrioid carcinoma tissue has the highest total carotenoid content (β-carotene, β-cryptoxanthin, lutein, zeaxanthin, canthaxanthin, epoxy-lutein, neoxanthin, violaxanthin and mutatoxanthin) and the highest percentage of the provitamin A group carotenoids (e.g.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, among the ovarian epithelial neoplasms, the endometrioid carcinoma tissue has the highest total carotenoid content (β-carotene, β-cryptoxanthin, lutein, zeaxanthin, canthaxanthin, epoxy-lutein, neoxanthin, violaxanthin and mutatoxanthin) and the highest percentage of the provitamin A group carotenoids (e.g. β-carotene) [17]. Thus, it is likely that carotenoids may have an influence on proliferation, differentiation and apoptosis of the ovarian carcinoma cells.…”

Section: Discussionmentioning

confidence: 99%

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Vitamin A family compounds, estradiol, and docetaxel in proliferation, apoptosis and immunocytochemical profile of human ovary endometrioid cancer cell line CRL-11731.

Czeczuga-Semeniuk

Bielawski²,

Lemancewicz³

et al. 2010

Folia Histochem Cytobiol.

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Endometrioid carcinoma represents approximately 10% of cases of the malignant ovarian epithelial tumors. According to literature, the vitamin A (carotenoids and retinoids) plays an essential role in cell proliferation, differentiation and apoptosis in both normal and neoplastic ovarian tissues. Apart from that, the retinoids alter a cytotoxic effect of chemiotherapeutics, i.e. docetaxel, on ovarian cancer cell lines. Retinoids act on cancer cells throughout different mechanism than taxanes, so they may be the potential candidates for the new treatment strategies of ovarian cancer.The aim of the study was to determine the effects of vitamin A family compounds (retinol, β-carotene, lycopene, all-trans -, 9-cis -and 13-cis retinoic acid) on the growth and proliferation of CRL-11731 endometrioid ovary cancer cell line and on docetaxel and estradiol activity in this culture. The assay was based on [ 3 H] thymidine incorporation and the proliferative activity of PCNA-and Ki 67-positive cells. The apoptotic index and expression of the Bcl-2 and p53 antigens in CRL-11731 cells were also studied. Among vitamin A family compounds retinol and carotenoids, but not retinoids, inhibited the growth of cancer cells in dose dependent manner. Only the concentration of 100 μM of docetaxel inhibited incorporation [ 3 H] thymidine into CRL-11731 cancer cells. Retinol (33.4%±8.5), carotenoids (β-carotene 20 μM 4.7%±2.9, 50 μM 2.2%±0.9; lycopene 10 μM 7.6%±0.8, 20 μM 5.2%±2.5, 50 μM 2.9%±1.2), and 13-cis retinoic acid (19.7%±2.2) combined with docetaxel (100 μM) significantly decreased the percentage of proliferating cells (p<0.0001). The antiproliferative action of lycopene alone and in combination with docetaxel was also confirmed in immunohistochemical examination (decreased the percentage of PCNA and Ki67 positive cells). Also retinol (10 μM) and lycopene (20 and 50 μM) combined with estradiol (0.01 μM) statistically decreased the percentage of proliferating cells compared to the control (p<0.0001) and estradiol (p<0.01, p<0.0001) group (63.5%±14.8, 61.0%±20.6, 15.0%±5.5 respectively). In our experiments, the compounds tested induced an apoptotic effect. Docetaxel and estradiol increased the percentage of apoptotic cells (71% apoptotic cells after administration of 10 μM all-trans retinoic acid combined with 0.01 μM estradiol, p<0.0001). β-carotene, lycopene and all-trans retinoic acid alone and in combination with docetaxel were found to influence the expression of bcl-2 and p53 antigen in the cells examined.The results of our study justified an important role of vitamin A in the pathophysiology of the ovarian endometrioid cancer.

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Section: Discussioncontrasting

confidence: 55%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Vitamin A family compounds, estradiol, and docetaxel in proliferation, apoptosis and immunocytochemical profile of human ovary endometrioid cancer cell line CRL-11731.

Czeczuga-Semeniuk

Bielawski²,

Lemancewicz³

et al. 2010

Folia Histochem Cytobiol.

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“…All other carotenoids tested had a K D above 1 μM. StARD1 is not present in the retina, but it is abundantly expressed in another carotenoid-rich tissue, the corpus luteum of mammalian ovary [38–41]. In contrast to StARD3, StARD1 exhibited a relatively low selectivity and affinity ( K D >1 μM) towards the various carotenoids tested.…”

Section: Resultsmentioning

confidence: 99%

Surface plasmon resonance (SPR) studies on the interactions of carotenoids and their binding proteins

Vachali¹,

Li²,

Nelson³

et al. 2012

Archives of Biochemistry and Biophysics

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The xanthophyll carotenoids lutein and zeaxanthin constitute the major carotenoids of the macular pigment in the human retina where they are thought to act in part to prevent light induced oxidative damage associated with age-related macular degeneration (AMD). The highly selective uptake of these pigments is mediated by specific carotenoid-binding proteins (GSTP1 and StARD3) recently identified in our laboratory. Carotenoids are hydrophobic in nature, so we first systematically optimized carotenoid preparations that are nano-dispersed in aqueous buffers, and then we used a new-generation surface plasmon resonance (SPR) protocol called FastStep™, which is significantly faster than conventional SPR assays. We have explored carotenoid-binding interactions of five proteins: human serum albumin (HSA), β-lactoglobulin (LG), steroidogenic acute regulatory domain proteins (StARD1, StARD3) and glutathione S- transferase Pi isoform (GSTP1). HSA and LG showed relatively weak interaction with carotenoids (KD > 1 μM). GSTP1 evidenced high affinity and specificity towards zeaxanthin and meso-zeaxanthin with KD values 0.14 ± 0.02 μM and 0.17 ± 0.02 μM, respectively. StARD3 expressed a relative high specificity towards lutein with a KD value of 0.59 ± 0.03 μM, whereas StARD1 exhibited a relatively low selectivity and affinity (KD > 1 μM) towards the various carotenoids tested.

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“…Epidemiologic and clinical studies have revealed that vitamin A and its derivatives (carotenoids and retinoids) can reduce the risk of many tumors, such as those of the ovary, 5 prostate, 6 skin, 7 and lungs 8 …”

Section: Introductionmentioning

confidence: 99%

Chemotherapeutic/chemopreventive role of retinoids in chemically induced skin carcinogenesis in albino mice

Bukhari¹,

Qureshi²,

Niazi³

et al. 2007

Int J Dermatology

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This study demonstrates that retinoids are effective as chemopreventive agents in premalignant lesions of the skin, but have a very weak chemotherapeutic role in malignant neoplasms. If retinoids are given at an early stage, they can cause regression of premalignant lesions of the skin. They are best administered both orally and parenterally. These agents should be recommended as they reduce the potential effects of carcinogenesis.

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Identification of carotenoids in ovarian tissue in women

Cited by 13 publications

References 0 publications

Vitamin A family compounds, estradiol, and docetaxel in proliferation, apoptosis and immunocytochemical profile of human ovary endometrioid cancer cell line CRL-11731.

Vitamin A family compounds, estradiol, and docetaxel in proliferation, apoptosis and immunocytochemical profile of human ovary endometrioid cancer cell line CRL-11731.

Surface plasmon resonance (SPR) studies on the interactions of carotenoids and their binding proteins

Chemotherapeutic/chemopreventive role of retinoids in chemically induced skin carcinogenesis in albino mice

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