Identification of Catechin Oligomers from Apple (Malus pumila cv. Fuji) in Matrix‐assisted Laser Desorption/Ionization Time‐of‐flight Mass Spectrometry and Fast‐atom Bombardment Mass Spectrometry

Ohnishi‐Kameyama, Mayumi; Yanagida, Akio; Kanda, Tomomasa; Nagata, Takayuki

doi:10.1002/(sici)1097-0231(19970115)11:1<31::aid-rcm784>3.3.co;2-k

Cited by 52 publications

(93 citation statements)

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“…Boyer and Liu (6) summarized various health benefits of apple phytochemicals; however, that review only showed functions of ripe apple and monomeric polyphenols. Unripe apples contain significantly higher levels of procyanidins than ripe apples (7); however, these procyanidins have not been used in foods yet. The procyanidins in unripe apple are 2-14mer oligomeric (−)-epicatechins, and they constitute over 60% of the total polyphenols in apples (7).…”

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confidence: 99%

“…Unripe apples contain significantly higher levels of procyanidins than ripe apples (7); however, these procyanidins have not been used in foods yet. The procyanidins in unripe apple are 2-14mer oligomeric (−)-epicatechins, and they constitute over 60% of the total polyphenols in apples (7). Scheme 1 shows structures of procyanidins in apple polyphenol (AP) from unripe apple.…”

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confidence: 99%

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Dose‐dependent hypocholesterolemic actions of dietary apple polyphenol in rats fed cholesterol

Osada

Suzuki

Kawakami

et al. 2006

Lipids

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The dose-dependent hypocholesterolemic and antiatherogenic effects of dietary apple polyphenol (AP) from unripe apple, which contains approximately 85% catechin oligomers (procyanidins), were examined in male Sprague-Dawley rats (4 wk of age) given a purified diet containing 0.5% cholesterol. Dietary AP at 0.5 and 1.0% levels significantly decreased the liver cholesterol level compared with that in the control (AP-free diet-fed) group. Dietary AP also significantly lowered the serum cholesterol level compared with that in the control group. However, the HDL cholesterol level was significantly higher in the 1.0% AP-fed group than in the control group. Accordingly, the ratio of HDL-cholesterol/total cholesterol was significantly higher in the 0.5% AP-fed group and 1.0% AP-fed group than in the control group. Moreover, the atherogenic indices in the 0.5 and 1.0% AP-fed groups were significantly lower than those in the control group. The activity of hepatic cholesterol 7alpha-hydroxylase tended to be increased by dietary AP in a dose-dependent manner. In accord with this observation, dietary AP increased the excretion of acidic steroids in feces. Dietary AP also significantly promoted the fecal excretion of neutral steroids in a dose-dependent manner. These observations suggest that dietary AP at a 0.5 or 1.0% level exerts hypocholesterolemic and antiatherogenic effects through the promotion of cholesterol catabolism and inhibition of intestinal absorption of cholesterol.

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confidence: 99%

mentioning

confidence: 99%

Dose‐dependent hypocholesterolemic actions of dietary apple polyphenol in rats fed cholesterol

Osada

Suzuki

Kawakami

et al. 2006

Lipids

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“…MALDI-TOFMS has been shown to be a valuable tool for the rapid analysis of plant-derived, small-molecular-weight compounds [19][20][21][22][23][24]. However, its use as an in vivo quantitation method has been limited.…”

Section: Discussionmentioning

confidence: 99%

“…THAP and DHB matrices have both been utilized for the MALDI analysis of small plant-derived compounds [19][20][21][22][23][24]. However, the critical factors in choosing a matrix for the analysis of small-molecular-weight compounds are the number and intensity of matrix peaks in the mass range of the compounds to be analyzed.…”

Section: Discussionmentioning

confidence: 99%

“…However, recent papers suggest that MALDI-TOFMS may be adapted for the study of molecules with masses of less than 500 Da, if it can be demonstrated that no matrix peaks occur in the mass region of interest. Accordingly, many researchers have utilized this technology for the identiWcation of many diVerent lowmass plant compounds such as anthocyanins [19], isoXavones [20], oligosaccharides [21], glucose [22], tannins [23], and Xavonol glycosides [24].…”

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Detection and quantitation of curcumin in mouse lung cell cultures by matrix-assisted laser desorption ionization time of flight mass spectrometry

May

Tourkina

Hoffman

et al. 2005

Analytical Biochemistry

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Effect of cationization reagents on the matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrum of Chinese gallotannins

Xiang¹,

Lin²,

Lin³

et al. 2007

J of Applied Polymer Sci

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In this study, Chinese gallotannins were characterized by MALDI-TOF MS, and effects of cationization reagents on the quality of spectra were investigated. The trideca- and tetradeca-galloyl glucoses were observed in Chinese gallotannins, which could not be detected in earlier studies. When Cs+ was used as the cationization reagent, Chinese gallotannins gave a relatively simple MALDI-TOF spectrum, three series of quasimolecular ions [M + Cs](+), [M + 2Cs-H](+), and [M + 3Cs-2H](+) and a series of metastable ion peaks with minimum abundance were detected. Selection of Na+ as the cationization reagent, additional three series of ion peaks including two patterns from the fragmentation and complex 2M adducts [2M + Na](+) can be distinguished. In the case of no deionization or addition of cationization reagent to the analyte/ matrix, naturally abundant Na+ and K+ as the cationization reagent, [M + Na](+) and [M + K](+) molecular ions both appeared in the complicated spectrum. Therefore, we conclude that cationization reagents affect the MALDI-TOF MS spectrum of Chinese gallotannins significantly. (c) 2007 Wiley Periodicals, fnc

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Identification of Catechin Oligomers from Apple (Malus pumila cv. Fuji) in Matrix‐assisted Laser Desorption/Ionization Time‐of‐flight Mass Spectrometry and Fast‐atom Bombardment Mass Spectrometry

Cited by 52 publications

References 0 publications

Dose‐dependent hypocholesterolemic actions of dietary apple polyphenol in rats fed cholesterol

Dose‐dependent hypocholesterolemic actions of dietary apple polyphenol in rats fed cholesterol

Detection and quantitation of curcumin in mouse lung cell cultures by matrix-assisted laser desorption ionization time of flight mass spectrometry

Effect of cationization reagents on the matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrum of Chinese gallotannins

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