Identification of Cell Surface Binding Sites for PC-Cell-Derived Growth Factor, PCDGF, (Epithelin/Granulin Precursor) on Epithelial Cells and Fibroblasts

Xia, Xianmin; Serrero, Ginette

doi:10.1006/bbrc.1998.8498

Cited by 37 publications

(31 citation statements)

References 23 publications

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“…However, several groups, including ours, have reported that the intact precursor was biologically active to stimulate the proliferation of fibroblast cells as well as epithelial cells (19,25,26). Cell surface binding sites for 125 I-PCDGF with an apparent molecular mass of 120 kDa have been characterized by Scatchard analysis and by affinity labeling of iodinated PCDGF in several cell lines of mesenchymal and epithelial origins (27). Study of teratoma-derived cell lines with increasing tumorigenicity has shown that PCDGF expression increased with tumorigenicity of the cells.…”

mentioning

confidence: 93%

Inhibition of PC cell-derived growth factor (PCDGF, epithelin/granulin precursor) expression by antisense PCDGF cDNA transfection inhibits tumorigenicity of the human breast carcinoma cell line MDA-MB-468

Serrero

2000

Proc. Natl. Acad. Sci. U.S.A.

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PC-cell derived growth factor (PCDGF) is an 88-kDa growth factor originally purified from the highly tumorigenic teratoma PC cell line and corresponds to the epithelin͞granulin precursor. In teratoma cells, PCDGF expression was shown to be essential for tumorigenicity. We have reported that PCDGF was expressed in estrogen receptorpositive (ER ؉ ) human mammary epithelial cells in an estrogen-dependent fashion. In this study,

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mentioning

confidence: 93%

Inhibition of PC cell-derived growth factor (PCDGF, epithelin/granulin precursor) expression by antisense PCDGF cDNA transfection inhibits tumorigenicity of the human breast carcinoma cell line MDA-MB-468

Serrero

2000

Proc. Natl. Acad. Sci. U.S.A.

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“…Taking into account the biological properties of proepithelin, it has been hypothesized that proepithelin could act through a ''classic'' membrane receptor(s), as for the other known growth factors. Thus far, a functional receptor has not been identified, although proepithelin (20) and epithelins (21) bind specifically to membrane proteins. Data from competitive binding experiments indicate that a long list of known growth factors and cytokines are unable to displace radiolabeled epithelin binding to its putative receptor (21), suggesting that the receptor for proepithelin is not a known tyrosine kinase receptor.…”

Section: Introductionmentioning

confidence: 99%

Proepithelin Promotes Migration and Invasion of 5637 Bladder Cancer Cells through the Activation of ERK1/2 and the Formation of a Paxillin/FAK/ERK Complex

Monami¹,

González²,

Hellman³

et al. 2006

Cancer Research

133

168

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The growth factor proepithelin (also known as progranulin, acrogranin, PC-derived growth factor, or granulin-epithelin precursor) is a secreted glycoprotein that functions as an important regulator of cell growth, migration, and transformation. Proepithelin is overexpressed in a great variety of cancer cell lines and clinical specimens of breast, ovarian, and renal cancer as well as glioblastomas. In this study, we have investigated the effects of proepithelin on bladder cancer cells using human recombinant proepithelin purified to homogeneity from 293-EBNA cells. Although proepithelin did not appreciably affect cell growth, it did promote migration of 5637 bladder cancer cells and stimulate in vitro wound closure and invasion. These effects required the activation of the mitogen-activated protein kinase pathway and paxillin, which upon proepithelin stimulation formed a complex with focal adhesion kinase and active extracellular signal-regulated kinase. Our results provide the first evidence for a role of proepithelin in stimulating migration and invasion of bladder cancer cells, and support the hypothesis that this growth factor may play a critical role in the establishment of the invasive phenotype. (Cancer Res 2006; 66(14): 7103-10)

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“…Neutrophils do express granulin, a growth factor that has structural and functional parallels to the family of epidermal growth factor-related peptides; 6-kD granulin peptides are stored in intracellular vesicles (Bateman et al 1990). Lung epithelial cells express receptors for the granulin/epithelin family of growth factors, suggesting a functional role for neutrophil-derived granulin in the repair of airway epithelium after resolution of acute inflammation (Xia and Serrero 1998).…”

Section: Discussionmentioning

confidence: 99%

Expression of the β6 Integrin Subunit Is Associated with Sites of Neutrophil Influx in Lung Epithelium

Miller

Barnett

Sheppard

et al. 2001

J Histochem Cytochem.

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S U M M A R YInhalation of ozone by Rhesus monkeys results in epithelial injury and granulocyte influx in both conducting airways and respiratory bronchioles. We have reported that ozone-induced neutrophil recruitment and subsequent epithelial repair can be inhibited in vivo with a CD18 antibody. The antibody-mediated effect is abrogated by local instillation of C5a (a CD18-independent neutrophil chemoattractant), thereby demonstrating a role for neutrophils in lung epithelial repair processes. As an extension of this study, we examined the effect of ozone and neutrophil influx on epithelial expression of the ␤ 6 integrin, an adhesion molecule associated with proliferation and repair. Expression of ␤ 6 integrin was determined by immunohistochemistry for ozone-exposed monkeys treated with either control immunoglobulins or a CD18 antibody. The tracheal epithelium of ozoneexposed monkeys treated with control immunglobulins expressed the ␤ 6 integrin. In contrast, the tracheal epithelium of ozone-exposed monkeys treated with CD18 antibody exhibited very low to undetectable expression of ␤ 6 integrin. In association with C5a instillation and neutrophil influx, ␤ 6 integrin was also observed in respiratory bronchiolar epithelium from both control and ozone-exposed animals. These findings cumulatively suggest that lung epithelial cell expression of ␤ 6 integrin is associated with sites of neutrophil recruitment.

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Identification of Cell Surface Binding Sites for PC-Cell-Derived Growth Factor, PCDGF, (Epithelin/Granulin Precursor) on Epithelial Cells and Fibroblasts

Cited by 37 publications

References 23 publications

Inhibition of PC cell-derived growth factor (PCDGF, epithelin/granulin precursor) expression by antisense PCDGF cDNA transfection inhibits tumorigenicity of the human breast carcinoma cell line MDA-MB-468

Inhibition of PC cell-derived growth factor (PCDGF, epithelin/granulin precursor) expression by antisense PCDGF cDNA transfection inhibits tumorigenicity of the human breast carcinoma cell line MDA-MB-468

Proepithelin Promotes Migration and Invasion of 5637 Bladder Cancer Cells through the Activation of ERK1/2 and the Formation of a Paxillin/FAK/ERK Complex

Expression of the β6 Integrin Subunit Is Associated with Sites of Neutrophil Influx in Lung Epithelium

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