Identification of chemical constituents of Selaginella doederleinii Hieron

Abstract

Abstract.To determine the chemical constituents of Selaginella doederleinii Hieron. The compounds were isolated and purified from the Ethyl Acetate Fraction from Ethanol Extract of Selaginella doederleinii Hieron by multiple chromatographic techniques including macroporous resin, polyamide, silica gel, Sephadex LH-20 and pre-HPLC. The structures were identified on the basis of thierphysico-chemical properties and spectroscopic data analyses

Aerial Parts of Selaginella doederleinii Hieron as an Anticancer Agent against Luminal A Breast Cancer (T47D) Cell Line

“…Other bioactive compounds in the EA fraction of S. doederleinii also showed anticancer activities, such as ferulic acid and vanillic acid. 19,35,36 Therefore, this study revealed that the EA fraction of S. doederleinii could specifically kill the T47D cancer cells without causing any toxicity to TIG-1 normal cells. The EA fraction of S. doederleinii was further examined using various assays.…”

“…Preparation of S. doederleinii extract and fractions S. doederleinii powder (500 grams) was extracted using the methods described by Brad et al (2017) and Djati et al (2021) with modifications. Briefly, the powder was macerated with 95% ethanol (1:10 ratio) for 24 h. 19,20 The mixture was filtrated using Whatman filter papers (Whatman Ltd., England) and evaporated using a rotatory evaporator at 50°C in vacuo. These maceration procedures were conducted twice.…”

Kandungan Senyawa Kimia Dan Bioaktivitas Dari Selaginella Doederleinii Hieron

“…Dua puluh senyawa berhasil diperoleh dari ekstrak tersebut. Struktur senyawa yang diperoleh antara lain : myristic acid, β- Citronellol,palmitic acid,stearic acid,physcion,emodin,chrysophanol,apigenin,amentoflavone,robustaflavone 7,4',heveaflavone,podocarpusflavone A,robustaflavone 4',adenosine, ferulic acid, syringate dan vanillic acid (Brad et al 2017). Ekstrak etanol 75% dari Selaginella mengandung senyawa doederflavones A, doederflavones B, unciflavones E, unciflavone D, unciflavone F, 6-(2-hydroxy-5-carboxyphenyl)-apigenin, apigenin, kaempferol, isoschaftoside, schaftoside, chromone, dan 2, 6, 8-trimethylchromone (Sivaraman et al 2013).…”