Identification of chicken cathelicidin-2 core elements involved in antibacterial and immunomodulatory activities

Dijk, Albert van; Molhoek, E. Margo; Veldhuizen, Edwin J.A.; Bokhoven, Johanna L.M. Tjeerdsma-van; Wagendorp, Eveline; Bikker, Floris J.; Haagsman, Henk P.

doi:10.1016/j.molimm.2009.05.019

Cited by 75 publications

(89 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, this peptide is non-haemolytic against erythrocytes [3]. In this study, we extended the earlier reported broad antibacterial activity with more bacteria, including bacteria often related to bioterrorist Substitutions of C1-15 Phe to Trp residues resulted in a peptide containing a large number of Arg and Trp residues.…”

Section: Discussionmentioning

confidence: 55%

“…Some HDPs are found to counteract the development of septic shock by neutralising endotoxin [10]. Although CATH-2 is able to inhibit the LPSinduced pro-inflammatory cytokine responses, the N-terminal part, C1-15, showed only moderate LPS-neutralising activities [3]. PheTrp substitutions in C1-15, resulting in peptide F2,5,12W, significantly increased the LPS-neutralising capacity.…”

Section: Discussionmentioning

confidence: 99%

“…The antimicrobial activity of C1-15 variants was evaluated against Gram-positive The antimicrobial activity of C1-15 and C1-15 variants was determined using colony counting assays as described previously [3]. M a n u s c r i p t 6…”

Section: Antibacterial Activitymentioning

confidence: 99%

“…Human PBMCs were isolated from buffy coats (Sanquin, Rotterdam, The Netherlands) as reported previously [3] and were seeded at 0.5  10 6 cells/well into a Corning Costar 96-well flat-bottom culture plate (Corning Inc., Corning, NY) in RPMI-1640 (Lonza BioWhittaker, Basel, Switzerland) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (FBS Gold; PAA, Pasching, Austria), 100 U/mL penicillin and 100 g/mL streptomycin (Lonza BioWhittaker).…”

Section: Isolation and Culture Of Peripheral Blood Mononuclear Cellsmentioning

confidence: 99%

“…We previously demonstrated that the N-terminal fragment of the HDP chicken cathelicidin-2 (CATH-2), namely the peptide C1-15, possesses broad-spectrum antibacterial activity while being non-haemolytic against erythrocytes and is therefore a promising lead for the development of novel antimicrobial drugs with broad-spectrum activity [3].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Chicken cathelicidin-2-derived peptides with enhanced immunomodulatory and antibacterial activities against biological warfare agents

Molhoek

Dijk

Veldhuizen

et al. 2010

International Journal of Antimicrobial Agents

Self Cite

View full text Add to dashboard Cite

Host defence peptides (HDPs) are considered to be excellent candidates for the development of novel therapeutic agents. Recently, it was demonstrated that the peptide C1-15, an N-terminal segment of chicken HDP cathelicidin-2, exhibits potent antibacterial activity while lacking cytotoxicity towards eukaryotic cells. In the present study, we report that C1-15 is active against bacteria such as Bacillus anthracis and

show abstract

Section: Discussionmentioning

confidence: 55%

Section: Discussionmentioning

confidence: 99%

Section: Antibacterial Activitymentioning

confidence: 99%

Section: Isolation and Culture Of Peripheral Blood Mononuclear Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Chicken cathelicidin-2-derived peptides with enhanced immunomodulatory and antibacterial activities against biological warfare agents

Molhoek

Dijk

Veldhuizen

et al. 2010

International Journal of Antimicrobial Agents

Self Cite

View full text Add to dashboard Cite

show abstract

Antiviral activity of selected cathelicidins against infectious bronchitis virus

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

The effect of structural modification of antimicrobial peptides on their antimicrobial activity, hemolytic activity, and plasma stability

Wang¹,

Zou²,

Na³

et al. 2021

Journal of Peptide Science

View full text Add to dashboard Cite

In this article, a series of modifications were made on an antimicrobial peptide F2,5,12W, including altering the amino acid sequence, introducing cysteine and other typical amino acids, developing peptide dimers via disulfide bonds, and conjugating with mPEG, in order to enhance the antimicrobial activity, plasma stability, and reduce the hemolytic activity of peptides. The results showed that mPEG conjugation could significantly improve the plasma stability and reduce the hemolytic activity of peptides, while the antimicrobial activity decreased meanwhile. However, altering the sequence of the peptide without changing its amino acid composition had little impact on its antimicrobial activity and plasma stability. The introduction of cysteine enhanced the plasma stability of peptides conspicuously, but at the same time, the increased hydrophobicity of peptides increased their hemolysis. The antimicrobial mechanism and cytotoxicity of the peptides with relatively high antimicrobial activity were also studied. In general, this study provided some ideas for the rational design and structure optimization of antimicrobial peptides.

show abstract

Identification of chicken cathelicidin-2 core elements involved in antibacterial and immunomodulatory activities

Cited by 75 publications

References 51 publications

Chicken cathelicidin-2-derived peptides with enhanced immunomodulatory and antibacterial activities against biological warfare agents

Chicken cathelicidin-2-derived peptides with enhanced immunomodulatory and antibacterial activities against biological warfare agents

Antiviral activity of selected cathelicidins against infectious bronchitis virus

The effect of structural modification of antimicrobial peptides on their antimicrobial activity, hemolytic activity, and plasma stability

Contact Info

Product

Resources

About