Identification of chicken, duck, pigeon and pig meat by species-specific markers of mitochondrial origin

Haunshi, Santosh; Basumatary, Rantu; Girish, P. S.; Doley, Sunil; Bardoloi, R.K.; Kumar, Ashok

doi:10.1016/j.meatsci.2009.06.026

Cited by 71 publications

(41 citation statements)

References 18 publications

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“…To guarantee the food authenticity, the development of analytical techniques to enable authorities and producers to check if the products are correctly described and labeled is necessary. The polymerase chain reaction is the most widely used molecular technique for the identification of the species origin in food, especially in meat products (11)(12)(13)(14)(15). In contrast, application of PCR-based techniques for the authentication of dairy products has been very limited.…”

Section: Discussionmentioning

confidence: 99%

Fraud Identification of Cow’s Milk in Buffalo’s Milk and It’s Products Using the Polymerase Chain Reaction

Zarei

Maktabi

Nasiri

2016

Jundishapur J Helath Sci

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Background: In the dairy industry, protection against species substitution or admixture is important for several reasons, including frequent human adverse reactions toward some species milk proteins, and trade and government regulations. Objectives: The objective of the present study was to assess the purity of buffalo milk and it's products offered as "pure buffalo" in the market. Methods: Using species-specific primers, a duplex polymerase chain reaction (PCR) assay was performed to detect the fraudulent addition of cow's milk to buffalo's milk and it's products. Results:The limit of detection of cow's milk in buffalo's milk, yogurt and cheese is 1, 2 and 4%, respectively. Undeclared presence of cow's milk was detected in 70% of the milk samples, 64% of the yogurt and 52% of the cheese samples. In 10% of the yogurt samples and 14% of the cheese samples, no apparent buffalo-related amplification product was observed, suggesting that cow's milk was entirely substituted for buffalo's milk in these samples. Conclusions: To avoid unfair competition and to assure consumers of accurate labeling, using this method is recommended for regulatory agencies.

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Section: Discussionmentioning

confidence: 99%

Fraud Identification of Cow’s Milk in Buffalo’s Milk and It’s Products Using the Polymerase Chain Reaction

Zarei

Maktabi

Nasiri

2016

Jundishapur J Helath Sci

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“…Later on, the same authors used the same target gene and sequencing as a tool for further development of PCR-RFLP methods for meat species differentiation (Girish et al, 2005(Girish et al, , 2007. In fact, sequencing of PCR products has been frequently used as an intermediate step during the development of several methods of PCR-RFLP (Fajardo et al, 2006;Rojas et al, 2009a), species-specific PCR (Haunshi et al, 2009;Santos et al, 2012), and real-time PCR (Laube et al, 2007;Santos et al, 2012;Kane and Hellberg, 2016).…”

Section: Pcr-sequencingmentioning

confidence: 99%

Advances in Authenticity Testing for Meat Speciation

Amaral

Meira

Oliveira

et al. 2016

Advances in Food Authenticity Testing

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“…Compared to other meat species, there are fewer poultry-specific assays for food authentication and quality control (Stamoulis et al 2010). Most poultry DNA-based assays utilize avian mitochondrial genes and do not have a nuclear DNA quantification component (Dalmasso et al 2004;Girish et al 2007;Stamoulis et al 2010;Kocher et al 1989;Herman 2004;Haunshi et al 2009;Girish et al 2011). The integration of species determination and nuclear DNA quantification into a single assay will streamline downstream genotyping analysis, and improve the quality of the DNA profiles while conserving reagents (Evans et al 2007;Lindquist et al 2011;Kanthaswamy et al 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Of the few poultry assays developed, most rely on mitochondrial DNA (mtDNA) genes as markers for detection (Girish et al 2007;Haunshi et al 2009;Herman 2001;Soares et al 2010). Since mtDNA occurs in high copy numbers in each cell and is able to withstand degradation and environmental challenges, it is well suited for use in food authentication assays.…”

Section: Introductionmentioning

confidence: 99%

A nuclear DNA-based species determination and DNA quantification assay for common poultry species

Satkoski

Premasuthan

et al. 2012

J Food Sci Technol

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DNA testing for food authentication and quality control requires sensitive species-specific quantification of nuclear DNA from complex and unknown biological sources. We have developed a multiplex assay based on TaqMan® realtime quantitative PCR (qPCR) for species-specific detection and quantification of chicken (Gallus gallus), duck (Anas platyrhynchos), and turkey (Meleagris gallopavo) nuclear DNA. The multiplex assay is able to accurately detect very low quantities of species-specific DNA from single or multispecies sample mixtures; its minimum effective quantification range is 5 to 50 pg of starting DNA material. In addition to its use in food fraudulence cases, we have validated the assay using simulated forensic sample conditions to demonstrate its utility in forensic investigations. Despite treatment with potent inhibitors such as hematin and humic acid, and degradation of template DNA by DNase, the assay was still able to robustly detect and quantify DNA from each of the three poultry species in mixed samples. The efficient species determination and accurate DNA quantification will help reduce fraudulent food labeling and facilitate downstream DNA analysis for genetic identification and traceability. ). The integration of species determination and nuclear DNA quantification into a single assay will streamline downstream genotyping analysis, and improve the quality of the DNA profiles while conserving reagents (Evans et al. 2007;Lindquist et al. 2011;Kanthaswamy et al. 2012). Many of the DNA-based food authentication assays are multiplexed end-point PCR assays (Dalmasso et al. 2004;Zha et al. 2010;Ghovvati et al. 2009;Rodriguez et al. 2003). A majority of these protocols require subsequent steps such as restriction enzyme digestion or gel electrophoresis for species identification after PCR. Endpoint PCR assays with a quantification component have been developed, but amplicon quantities are typically determined using imaging software analysis of fluorescence intensities of electrophoresis gel bands (Soares et al. 2010).Like end-point PCR, real-time quantitative PCR (qPCR) technology allows multiplexing, and is amenable to detecting multiple DNA targets that allow the simultaneous identification of DNA originating from multiple species. Moreover, the real-time DNA quantification feature of qPCR technology facilitates the efficient and accurate quantification of template DNA. While simplifying data collection and analysis, qPCR's ability to amplify, identify, and quantify in a single step effectively minimizes turnaround time and exposure to contamination and errors ).Electronic supplementary material The online version of this article

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Identification of chicken, duck, pigeon and pig meat by species-specific markers of mitochondrial origin

Cited by 71 publications

References 18 publications

Fraud Identification of Cow’s Milk in Buffalo’s Milk and It’s Products Using the Polymerase Chain Reaction

Fraud Identification of Cow’s Milk in Buffalo’s Milk and It’s Products Using the Polymerase Chain Reaction

Advances in Authenticity Testing for Meat Speciation

A nuclear DNA-based species determination and DNA quantification assay for common poultry species

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