Identification of chondrocyte proliferation following laser irradiation, thermal injury, and mechanical trauma

Wong, Brian J. F.; Pandhoh, Nidhi; Truong, Mai Thy; Diaz, Sergio; Chao, K.; Hou, Stephen; Gardiner, David M.

doi:10.1002/lsm.20180

Cited by 25 publications

(23 citation statements)

References 13 publications

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“…∼100 μs), generates mechanical deformations conveyed at cellular level through the ECM integrins-cytoskeleton network. In other words, laser-induced modifications of the ECM component may in turn give rise to mechanical stress on the cellular component (Zati et al 2004;Ninomiya et al 2003;Wong et al 2005;Rossi et al 2010).…”

Section: Discussionmentioning

confidence: 94%

An in Vitro Study on Tissue Repair: Impact of Unloading on Cells Involved in the Remodelling Phase

Monici

Cialdai

Romano

et al. 2011

Microgravity Sci. Technol.

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The number of astronauts involved in longlasting missions and extra-vehicular activities is going to increase in the future. Consequently, the chance of injury due to traumatic events or unexpected emergency surgery will also increase and medical evacuation times to earth will be prolonged. Hence, the need to address requirements for surgery and trauma care in non terrestrial environments will be a priority. Tissue repair in weightlessness should therefore be regarded as a major issue not enough studied to date. Wound healing is a complex multi-step process, crucial to the survival of the organism. It starts with an inflammatory phase followed by a remodelling phase. During repair, the extracellular matrix (ECM) is sequentially remodelled by the concerted action of different cell types, in order to rebuild a functional tissue. The available literature concerning wound healing with mechanical unloading presents controversial results. However, many studies indicate impairment of the healing processes. Here we present a study on the behaviour of cells involved in the remodelling phase of repair, e.g. fibroblasts and endothelial cells, in response to microgravity (μg). In particular, their adhesion/migration, cytoskeleton organization, production of ECM molecules and receptors have been investigated. Cell response to pulsed Nd:YAG laser irradiation has also been investigated in order to evaluate the possibility to use laser irradiation for counteracting the effect of μg on wound healing. In μg, we observed alterations in production/assembling of ECM molecules. Increased fibronectin (FN) and laminin (LM) could be the cause for impaired ECM rebuilding and altered cell adhesion/migration. Treatment with Nd:YAG laser pulses induced organized fibrillogenesis and favoured endothelial cell spreading and monolayer formation. These findings open the way for a better understanding of tissue repair mechanisms in space and future clinical applications on earth.

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Section: Discussionmentioning

confidence: 94%

An in Vitro Study on Tissue Repair: Impact of Unloading on Cells Involved in the Remodelling Phase

Monici

Cialdai

Romano

et al. 2011

Microgravity Sci. Technol.

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“…The specimens were trimmed and the perichondrium was removed from the surface of the cartilage by gentle scraping using tissue paper [16]. Specimens were stored in saline solution until irradiation, which occurred within 1 hour of harvest.…”

Section: Methodsmentioning

confidence: 99%

Analysis of Nd:YAG laser‐mediated thermal damage in rabbit nasal septal cartilage

Protsenko

Zemek

et al. 2007

Lasers Surg Med

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“…The advantage of the laser effect on chondrocytes proliferation compared to other thermal, mechanical, and chemical effects was demonstrated in Ref. 52. No evidence of chondrocyte DNA replication was observed in tissues heated using nonlaser methods, grown in TGF-β-contained media, or mechanically traumatized.…”

Section: Targets For Laser Effect Possible Types Of Cartilage Responmentioning

confidence: 97%

Laser-induced regeneration of cartilage

et al. 2011

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Abstract. Laser radiation provides a means to control the fields of temperature and thermo mechanical stress, mass transfer, and modification of fine structure of the cartilage matrix. The aim of this outlook paper is to review physical and biological aspects of laser-induced regeneration of cartilage and to discuss the possibilities and prospects of its clinical applications. The problems and the pathways of tissue regeneration, the types and features of cartilage will be introduced first. Then we will review various actual and prospective approaches for cartilage repair; consider possible mechanisms of laser-induced regeneration. Finally, we present the results in laser regeneration of joints and spine disks cartilages and discuss some future applications of lasers in regenerative medicine. C 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).

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Identification of chondrocyte proliferation following laser irradiation, thermal injury, and mechanical trauma

Cited by 25 publications

References 13 publications

An in Vitro Study on Tissue Repair: Impact of Unloading on Cells Involved in the Remodelling Phase

An in Vitro Study on Tissue Repair: Impact of Unloading on Cells Involved in the Remodelling Phase

Analysis of Nd:YAG laser‐mediated thermal damage in rabbit nasal septal cartilage

Laser-induced regeneration of cartilage

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