Identification of Circular RNAs in the Anterior Pituitary in Rats Treated with GnRH

Guo, Haixiang; Yuan, Bao; Su, Mengting; Zheng, Yi; Zhang, Jinyu; Han, Dong-Xu; Wang, Hao-Qi; Huang, Yongping; Jiang, Hao; Zhang, Jiabao

doi:10.3390/ani11092557

Cited by 3 publications

(5 citation statements)

References 78 publications

(88 reference statements)

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“…Our previous research revealed significant differences in methylation levels in the adenohypophyses of rats after GnRH treatment [ 24 ]. In addition, 14 circRNAs were found to be significantly differentially expressed in the rat adenohypophysis [ 22 ]. However, the overall regulatory effects of GnRH on the expression of other ncRNAs and mRNAs in the rat adenohypophysis have not been reported.…”

Section: Discussionmentioning

confidence: 99%

“…The animals used in the experiments were 8-week-old male Sprague Dawley (SD) rats, provided by the laboratory Animal Center of Jilin University. The grouping strategy used for this study has been described previously [ 22 ]. Briefly, 12 SD rats were equally divided into four groups: two control groups and two experimental groups.…”

Section: Methodsmentioning

confidence: 99%

“…The rat is a common experimental animal model for research on the effect of GnRH on the pituitary gland. Various studies have used rat experimental animal models to explore the expression changes of gonadotropins in the adenohypophysis after a GnRH treatment [ 22 , 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

“…Related studies have reported that GnRH treatment can alter microRNA (miRNA) expression profiles in mouse LβT2 gonadotropic cells and porcine anterior pituitary cells [ 9 , 27 ]. Our previous study also revealed that after GnRH treatment, the levels of circRNAs in the rat adenohypophysis are changed, and 14 circRNAs are significantly differentially expressed [ 22 ]. NcRNAs in the pituitary can play important roles.…”

Section: Introductionmentioning

confidence: 99%

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Sequencing of the Pituitary Transcriptome after GnRH Treatment Uncovers the Involvement of lncRNA-m23b/miR-23b-3p/CAMK2D in FSH Synthesis and Secretion

Zhao

Song

Zheng

et al. 2023

Genes

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The pituitary gland is a key participant in the hypothalamic–pituitary–gonadal axis, as it secretes a variety of hormones and plays an important role in mammalian reproduction. Gonadotrophin-releasing hormone(GnRH) signaling molecules can bind to GnRH receptors on the surfaces of adenohypophysis gonadotropin cells and regulate the expression of follicle-stimulating hormone(FSH) and luteinizing hormone(LH) through various pathways. An increasing number of studies have shown that noncoding RNAs mediate the regulation of GnRH signaling molecules in the adenohypophysis. However, the expression changes and underlying mechanisms of genes and noncoding RNAs in the adenohypophysis under the action of GnRH remain unclear. In the present study, we performed RNA sequencing (RNA-seq) of the rat adenohypophysis before and after GnRH treatment to identify differentially expressed mRNAs, lncRNAs, and miRNAs. We found 385 mRNAs, 704 lncRNAs, and 20 miRNAs that were significantly differentially expressed in the rat adenohypophysis. Then, we used a software to predict the regulatory roles of lncRNAs as molecular sponges that compete with mRNAs to bind miRNAs, and construct a GnRH-mediated ceRNA regulatory network. Finally, we enriched the differentially expressed mRNAs, lncRNA target genes, and ceRNA regulatory networks to analyze their potential roles. Based on the sequencing results, we verified that GnRH could affect FSH synthesis and secretion by promoting the competitive binding of lncRNA-m23b to miR-23b-3p to regulate the expression of Calcium/Calmodulin Dependent Protein Kinase II Delta(CAMK2D). Our findings provide strong data to support exploration of the physiological processes in the rat adenohypophysis under the action of GnRH. Furthermore, our profile of lncRNA expression in the rat adenohypophysis provides a theoretical basis for research on the roles of lncRNAs in the adenohypophysis.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sequencing of the Pituitary Transcriptome after GnRH Treatment Uncovers the Involvement of lncRNA-m23b/miR-23b-3p/CAMK2D in FSH Synthesis and Secretion

Zhao

Song

Zheng

et al. 2023

Genes

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“…Circ-ZNF609 regulates nasopharyngeal carcinoma cell growth via modulating miR-188 expression [50]. Some studies have identified circRNAs in the pituitary gland [51][52][53][54]. At present, there have been few reports of circRNA regulating GH.…”

Section: Discussionmentioning

confidence: 99%

Rno_circ_0001004 Acts as a miR-709 Molecular Sponge to Regulate the Growth Hormone Synthesis and Cell Proliferation

Xiong

Zhang

Wang

et al. 2022

IJMS

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(1) Background: As a novel type of non-coding RNA with a stable closed-loop structure, circular RNA (circRNA) can interact with microRNA (miRNA) and influence the expression of miRNA target genes. However, circRNA involved in pituitary growth hormone (GH) regulation is poorly understood. Our previous study revealed protein kinase C alpha (PRKCA) as the target gene of miR-709. Currently, the expression and function of rno_circRNA_0001004 in the rat pituitary gland is not clarified; (2) Methods: In this study, both bioinformatics analysis and dual-luciferase report assays showed a target relationship between rno_circRNA_0001004 and miR-709. Furthermore, the rno_circRNA_0001004 overexpression vector and si-circ_0001004 were constructed and transfected into GH3 cells; (3) Results: We found that rno_circRNA_0001004 expression was positively correlated with the PRKCA gene and GH expression levels, while it was negatively correlated with miR-709. In addition, overexpression of rno-circ_0001004 also promoted proliferation and relieved the inhibition of miR-709 in GH3 cells; (4) Conclusions: Our findings show that rno_circ_0001004 acts as a novel sponge for miR-709 to regulate GH synthesis and cell proliferation, and are the first case of discovery of the regulatory role of circRNA_0001004 in pituitary GH.

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Comprehensive Analysis of Differentially Expressed CircRNAs in the Ovaries of Low- and High-Fertility Sheep

Wang

Chen

Zhang

et al. 2023

Animals

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CircRNAs are essential in regulating follicle growth and development and the female reproductive system at multiple levels. However, the molecular mechanism by which circRNAs regulate reproduction in sheep is unclear and requires further exploration. In this study, RNA sequencing was performed to reveal the circRNA expression profiles in the ovaries of Cele black sheep and Hetian sheep during estrus. Analysis of the number of circRNAs in their host genes revealed that 5031 genes could produce 20,835 circRNAs. Among the differentially expressed circRNAs (DEcircRNA), 75 were upregulated, and 105 were downregulated. Functional enrichment analysis showed that the host genes of DEcircRNA were involved in several pathways, including the MAPK and Hippo signaling pathway. In addition, we constructed a subnetwork of competitive endogenous RNA (ceRNA) containing 4 mRNAs, 4 microRNAs (miRNAs), and 10 circRNAs, potentially related to follicle development. Functional circRNAs (e.g., novel_circ_0003851, novel_circ_0015526, novel_circ_0008117) were found to act as ceRNAs for follicle growth and development-related mRNAs (CUEDC1, KPNB1, ZFPM2) by sponging functional miRNAs (miR-29a, miR-29b, miR-17-5p). Finally, through an RNA pull-down assay, oar-miR-125b was selected and confirmed as the target miRNA of novel-circ-0041512. We analyzed the overall expression of circRNAs in sheep ovaries. Further, we explored the potential mechanisms underlying the circRNA functions, providing a theoretical basis for the genetic progress of reproductive traits in sheep.

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Identification of Circular RNAs in the Anterior Pituitary in Rats Treated with GnRH

Cited by 3 publications

References 78 publications

Sequencing of the Pituitary Transcriptome after GnRH Treatment Uncovers the Involvement of lncRNA-m23b/miR-23b-3p/CAMK2D in FSH Synthesis and Secretion

Sequencing of the Pituitary Transcriptome after GnRH Treatment Uncovers the Involvement of lncRNA-m23b/miR-23b-3p/CAMK2D in FSH Synthesis and Secretion

Rno_circ_0001004 Acts as a miR-709 Molecular Sponge to Regulate the Growth Hormone Synthesis and Cell Proliferation

Comprehensive Analysis of Differentially Expressed CircRNAs in the Ovaries of Low- and High-Fertility Sheep

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