Identification of clinically relevant Corynebacterium strains by Api Coryne, MALDI-TOF-mass spectrometry and molecular approaches

Alibí, Sana; Ferjani, Asma; Gaillot, Olivier; Marzouk, Manel; Courcol, René; Boukadida, Jalel

doi:10.1016/j.patbio.2015.07.007

Cited by 42 publications

(32 citation statements)

References 28 publications

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“…A review of all Corynebacterium species isolated from culture and identified by MALDI-TOF MS and 16S RNA sequencing from 1 June 2013 to 1 July 2015 is displayed in Table 1, demonstrating a total of 308 corynebacteria identified to the species level. Of note, half of these specimens were identified as Corynebacterium striatum, which is increasingly being recognized as a clinically significant corynebacterium (6). An additional 105 specimens were identified as Corynebacterium species but were unable to be identified to the species level by MALDI-TOF MS, which is not surprising as not all species are in the MALDI-TOF MS database.…”

Section: Discussionmentioning

confidence: 99%

The Brief Case: Recurrent Granulomatous Mastitis Due to Corynebacterium kroppenstedtii

et al. 2016

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A 34-year-old female from Mexico with a history of granulomatous mastitis of the left breast due to Corynebacterium kroppenstedtii presented to a surgery clinic with progressive pain, erythema, and swelling of the right breast. Fourteen months prior, she had noted a painful mass in her left breast that progressively worsened over 3 months and did not improve with a course of doxycycline. She was referred to an outpatient surgery clinic, where a breast ultrasound demonstrated a predominant 4-cm by 4-cm collection consistent with an abscess; 5 ml of serosanguinous fluid was aspirated. A Gram stain demonstrated 3ϩ polymorphonuclear leukocytes and 1ϩ Gram-positive rods; the aspirate grew C. kroppenstedtii on chocolate agar, as well as on anaerobic brain heart infusion agar and anaerobic Columbia agar with colistin and nalidixic acid (CNA). It was identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) (bioMérieux, Durham, North Carolina, USA). The patient continued to have breast tenderness and erythema over the next month despite multiple antibiotics (doxycycline, clindamycin, and amoxicillin-clavulanic acid) and another ultrasoundguided breast aspiration; thus, she underwent incision and drainage of the left breast, which resulted in operative findings notable for inflamed tissue with many pus-filled cavities. A Gram stain demonstrated 2ϩ polymorphonuclear leukocytes with no organisms; aerobic, anaerobic, and mycobacterial cultures demonstrated no growth. Surgical pathology demonstrated granulomatous inflammation with associated acute and chronic inflammation and fat necrosis; fungal and mycobacterial special stains were negative for microorganisms. An infectious disease specialist was consulted, and he felt that the abscesses persisted because they were never adequately drained and because the patient did not receive long-enough courses of antibiotic therapy. She was given a 4-week course of doxycycline and amoxicillin-clavulanic acid, and her abscesses resolved.However, 1 year later, she noted progressive pain, erythema, and swelling of the contralateral breast. A right breast ultrasound demonstrated multiple abscesses, and 7 ml of purulent fluid was aspirated. A Gram stain demonstrated 3ϩ polymorphonuclear leukocytes with no organisms; the aspirate grew Ͻ10 colonies of C. kroppenstedtii on CNA agar. Her abscesses did not improve despite multiple antibiotics (dicloxacillin, clindamycin, trimethoprim-sulfamethoxazole, doxycycline, amoxicillin-clavulanic acid, and ciprofloxacin), a course of prednisone, two ultrasound-guided breast aspirations, and two incision and drainage procedures over the next 4 months. C. kroppenstedtii was isolated from both breast aspirations on chocolate and CNA agar, and after the first incision and drainage procedure on CNA agar. Surgical pathology of the second incision and drainage procedure demonstrated fat necrosis with prominent histiocytic infiltration, acute and chronic inflammation, and reactive fibrosis; no granuloma formation w...

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Section: Discussionmentioning

confidence: 99%

The Brief Case: Recurrent Granulomatous Mastitis Due to Corynebacterium kroppenstedtii

et al. 2016

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“…26 Moreover, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), mainly based on Bruker systems, has been established for the identification of clinical Corynebacterium species and has since successfully complemented classical biochemical approaches. [27][28][29][30][31] Using MALDI-TOF MS as described previously, the two coryneform isolates investigated by the present authors gave significant scores for C. kroppenstedtii of 1.990 and 1.780, values considered accurate enough for species identification. 27,31 Determination of the 16S rRNA gene sequences and of the hypervariable region of the rpoB gene are the reference methods for taxonomic identification of corynebacteria.…”

Section: Microbiological Identification Of C Kroppenstedtiimentioning

confidence: 84%

A microbiological and clinical review on Corynebacterium kroppenstedtii

Tauch

Fernández-Natal

Soriano³

2016

International Journal of Infectious Diseases

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The genus Corynebacterium represents a taxon of Gram-positive bacteria with a high G+C content in the genomic DNA. Corynebacterium kroppenstedtii is an unusual member of this taxon as it lacks the characteristic mycolic acids in the cell envelope. Genome sequence analysis of the C. kroppenstedtii type strain has revealed a lipophilic (lipid-requiring) lifestyle and a remarkable repertoire of carbohydrate uptake and utilization systems. Clinical isolates of C. kroppenstedtii have been obtained almost exclusively from female patients and mainly from breast abscesses and cases of granulomatous mastitis. However, the role of C. kroppenstedtii in breast pathologies remains unclear. This review provides a comprehensive overview of the taxonomy, microbiology, and microbiological identification of C. kroppenstedtii, including polyphasic phenotypic approaches, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and the use of 16S rRNA gene sequencing. A clinical review presents reported cases, various antimicrobial treatments, antibiotic susceptibility assays, and antibiotic resistance genes detected during genome sequencing. C. kroppenstedtii must be considered a potential opportunistic human pathogen and should be identified accurately in clinical laboratories.

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“…; Alibi et al . ). Several studies showed that treatment with formic acid resulted in higher level identification compared to direct colony testing (Theel et al .…”

Section: Automated Identification Systemsmentioning

confidence: 97%

Contemporary microbiology and identification ofCorynebacteriaspp. causing infections in human

Zasada

Mosiej

2018

Lett Appl Microbiol

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Significance and Impact of the Study: The number of opportunistic infections caused by Corynebacteria is increasing due to increase in number of immunocompromised patients. New Corynebacterium species and new human infections, caused by this group of bacteria, has been described recently. However, identification of Corynebacteria is still a challenge despite application of sophisticated laboratory methods. In the study we present possibilities and limitations of various commercial systems for identification of Corynebacteria. AbstractThe Corynebacterium is a genus of bacteria of growing clinical importance. Progress in medicine results in growing population of immunocompromised patients and growing number of infections caused by opportunistic pathogens. A new infections caused by new Corynebacterium species and species previously regarded as commensal micro-organisms have been described. Parallel with changes in Corynebacteria infections, the microbiological laboratory diagnostic possibilities are changing. But identification of this group of bacteria to the species level remains difficult. In the paper, we present various manual, semiautomated and automated assays used in clinical laboratories for Corynebacterium identification, such as API Coryne, RapID CB Plus, BBL Crystal Gram Positive ID System, MICRONAUT-RPO, VITEK 2, BD Phoenix System, Sherlock Microbial ID System, MicroSeq Microbial Identification System, Biolog Microbial Identification Systems, MALDI-TOF MS systems, polymerase chain reaction (PCR)-based and sequencing-based assays. The presented assays are based on various properties, like biochemical tests, specific DNA sequences, composition of cellular fatty acids, protein profiles and have specific limitations.

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Identification of clinically relevant Corynebacterium strains by Api Coryne, MALDI-TOF-mass spectrometry and molecular approaches

Cited by 42 publications

References 28 publications

The Brief Case: Recurrent Granulomatous Mastitis Due to Corynebacterium kroppenstedtii

The Brief Case: Recurrent Granulomatous Mastitis Due to Corynebacterium kroppenstedtii

A microbiological and clinical review on Corynebacterium kroppenstedtii

Contemporary microbiology and identification ofCorynebacteriaspp. causing infections in human

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