Identification of clonal complexes of Mycobacterium bovis in Brazil

Sales, Érica Bravo; Alencar, Andrea Padilha de; Hodon, Mikael Arrais; Filho, Paulo Martins Soares; Filho, Antônio Francisco de Souza; Lage, Andrey Pereira; Heinemann, Marcos Bryan; Júnior, Antônio Augusto Fonseca

doi:10.1007/s00203-019-01674-4

Cited by 6 publications

(3 citation statements)

References 27 publications

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“…In this study, Eu1 was found in 13.5 % (10/74) of samples, which is similar to previous estimates of prevalence in Brazil that ranged between 14.4 % (13/90) and 16 % (6/36) [ 83, 84 ]. The prevalence of Eu2, found in 64/74 (86.5 %) of isolates in this study, was similar to the prevalence identified in the states of Minas Gerais, São Paulo, Mato Grosso, Mato Grosso do Sul, Goiás, Tocantins and Pará, where the prevalence was 81.1 % (73/90) [ 90 ].…”

Section: Discussionsupporting

confidence: 86%

Genomic and temporal analyses of Mycobacterium bovis in southern Brazil

et al. 2021

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Mycobacterium bovis is a causal agent of bovine tuberculosis (bTB), one of the most important diseases currently facing the cattle industry worldwide. Tracing the source of M. bovis infections of livestock is an important tool for understanding the epidemiology of bTB and defining control/eradication strategies. In this study, whole genome sequencing (WGS) of 74 M . bovis isolates sourced from naturally infected cattle in the State of Rio Grande do Sul (RS), southern Brazil, was used to evaluate the population structure of M. bovis in the region, identify potential transmission events and date the introduction of clonal complex (CC) European 2 (Eu2). In silico spoligotyping identified 11 distinct patterns including four new profiles and two CCs, European 1 (Eu1) and Eu2. The analyses revealed a high level of genetic diversity in the majority of herds and identified putative transmission clusters that suggested that within- and between-herd transmission is occurring in RS. In addition, a comparison with other published M. bovis isolates from Argentina, Brazil, Paraguay and Uruguay demonstrated some evidence for a possible cross-border transmission of CC Eu1 into RS from Uruguay or Argentina. An estimated date for the introduction of CC Eu2 into RS in the middle of the 19th century correlated with the historical introduction of cattle into RS to improve existing local breeds. These findings contribute to the understanding of the population structure of M. bovis in southern Brazil and highlight the potential of WGS in surveillance and helping to identify bTB transmission.

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Section: Discussionsupporting

confidence: 86%

Genomic and temporal analyses of Mycobacterium bovis in southern Brazil

et al. 2021

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“…Generally, in Latin America, it is common to nd both European Clonal Complex 1 and 2 (Eu1 and Eu2, respectively) well distributed as a consequence of the introduction of cattle from the United Kingdom and Iberian Peninsula during the colonial period (16). Conversely, there have been no reports of the African 1 and 2 complexes (Af1 and Af2, respectively) in the American continent to date (17)(18)(19)(20)(21)(22)(23). A fourth complex, proposed as European 3 (24) (also known as the "BCG-like" complex), was identi ed in the United States in isolates from the early 1990s (20), but no other reports exist from other regions of the Americas.…”

Section: Introductionmentioning

confidence: 99%

Whole-genome sequencing to investigate Mycobacterium bovis strains circulating in the Dominican Republic

Perea¹,

Ceres

Durán

et al. 2023

Preprint

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Background Bovine tuberculosis (bTB) by Mycobacterium bovis (M. bovis) is endemic in cattle in Latin America, and even though government programs exist for the control and eradication of the disease, efforts are hindered by the limited resources available for farmer compensation and program operation. Epidemiological surveillance and strain characterization are key for understanding disease dynamics, for which whole genome sequencing (WGS) is a powerful tool. Currently, there is limited information regarding bTB in the Caribbean, so the aim of this study was to use WGS to characterize M. bovis strains circulating in the Dominican Republic (DR). Results A total of 81 M. bovis isolates were obtained from cattle from different regions were sequenced and analyzed. Phylogenetic and ancestral reconstruction/temporal analyses were performed to investigate M. bovis genetic diversity, population structure, and potential dates of introduction into the Dominican Republic. Three main lineages were identified: DR1, DR2 (consisting of subgroups DR2a, DR2b, and DR2c), and DR3. Cluster analysis revealed at least four introductions of M. bovis during the 19th century and the first half of the 20th century. The most recent common ancestors to the DR lineages were linked to the United States, Mexico, Argentina, and Brazil, from where historical imports of cattle have occurred. Population structure analyses indicated that M. bovis is largely panmictic in the Dominican Republic, with no relationship between geographic location and genotype. Conclusion The current study provides important information regarding the origin of M. bovis strains circulating in the Dominican Republic, its genetic diversity and spread in the local cattle herds, providing a frame of reference for continuing M. bovis surveillance. Finally, this study significantly contributes to the worldwide M. bovis framework.

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“…So, plants can be considered as the source of potential chemical substances, only part of which has been exploited. These potentially useful chemicals can be used not only as a drug but also as an unrivaled model as the starting point for making pharmaceutical analogues, and as an interesting tool for understanding better biological phenomena [10][11][12][13]. Rosmarinus officinalis belongs to the Lamiacea family and is widely known as a medicinal herb among many countries.…”

Section: Graphical Abstract Introductionmentioning

confidence: 99%

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Jafari-Sales

Hossein-Nezhad

2020

J. Med. Chem. Sci.

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Treatment of bacterial infections with chemicals has led to drug resistance. Therefore, research to replace herbal treatments with less side effects is of a great importance. Therefore, this study aims at investigating the antimicrobial effects of methanolic extract of Rosmarinus officinalis on some gram positive and gram negative bacteria. In this research study, after collecting the plant and confirming its scientific name, R. officinalis extract was prepared using Soxhlet extractor method at the concentrations of 20-400 mg/mL and the antimicrobial effects of the extract using agar well diffusion and determination of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) from dilution method against standard bacteria of Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa. Methanolic extract of R . officinalis plant has an inhibitory effect on P. aeruginosa, E. coli and S. aureus so that it has the highest sensitivity to methanolic extracts of R. officinalis in P. aeruginosa with a 19.8 mm the zone of growth inhibition and the lowest sensitivity to S. aureus with the zone of growth inhibition 14.4 mm. The results of this study showed that R. officinalis extract has a significant effect on tested bacteria, and further research is required to identify, quantify, and purify its effective compounds.

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Identification of clonal complexes of Mycobacterium bovis in Brazil

Cited by 6 publications

References 27 publications

Genomic and temporal analyses of Mycobacterium bovis in southern Brazil

Genomic and temporal analyses of Mycobacterium bovis in southern Brazil

Whole-genome sequencing to investigate Mycobacterium bovis strains circulating in the Dominican Republic

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