Identification of Clostridium spp. derived from a sheep and cattle slaughterhouse by matrix-assisted laser desorption and ionization-time of flight mass spectrometry (MALDI-TOF MS) and 16S rDNA sequencing

Bakhtiary, Farzaneh; Sayevand, Hamid Reza; Remely, Marlene; Hippe, Berit; Indra, Alexander; Hosseini, Hedayat; Haslberger, Alexander G.

doi:10.1007/s13197-018-3255-2

Cited by 10 publications

(5 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our study, based on the 16S rRNA sequence analysis of clostridial strains isolated from silage, raw milk, and Kashar cheese, C. sporogenes was determined to be the most common species among all of our isolates. The findings of this study are consistent with the literature data indicating a high C. sporogenes prevalence, according to some researchers (Garde et al, 2011;Bakhtiary et al, 2018;Oliveira et al, 2018).…”

Section: Identification Results Of Clostridium Sppsupporting

confidence: 93%

Determination of Endospore Structure and Volatile Compounds of Clostridial Species During Sporulation

Ertürkmen

Öner

2023

Gıda

View full text Add to dashboard Cite

u çalışmada, çeşitli örneklerden izole edilen ve peynirde geç şişme kusuruna neden olan clostridial türlerin sporulasyon süresince endospor yapısal ve uçucu bileşikleri araştırılmıştır. Gram boyama, katalaz ve oksidaz testi, anaerobik gelişim testleri ile morfolojik olarak tanımlanan türler 16S rRNA gen sekans analizi ile doğrulanmıştır. Sporulasyon süresince, yüksek gaz üretim kapasitesine sahip suşlar Taramalı Elektron Mikroskobu (SEM) ve gaz kromatografisi-kütle spektrometresi (GC-MS) ile analiz edilmiştir. Sporulasyon sonunda bütanol, pentanol, ethanol, hexanol, proponol, oktanol gibi alkol bileşikleri; sülfit, ester ve asit grubu bileşikler öne çıkan bileşenlerdendir. Toplam bağıl yüzde alanı >%50 sülfit grubu bileşiğe sahip olan Clostridium sporogenes suşlarının hücre duvarı kalıntıları ve endospor yapısal bileşenleri; bütanol üretim düzeyi yüksek olan Clostridium butyricum suşlarına göre daha yoğun şekilde gözlemlenmiştir. Bu çalışmadan elde edilen bilgiler geç şişme etmeni clostridial türlerin endospor yapısal ve uçucu bileşiklerinin tespitine yönelik çalışmalara kolaylık sağlayacaktır.

show abstract

Section: Identification Results Of Clostridium Sppsupporting

confidence: 93%

Determination of Endospore Structure and Volatile Compounds of Clostridial Species During Sporulation

Ertürkmen

Öner

2023

Gıda

View full text Add to dashboard Cite

show abstract

“…Then, 100 µL was cultured on Brazier’s agar without added selective agents (D-cycloserine and cefoxitin) and on 7% sheep blood agar, incubated at 37 °C for 48 h/72 h in an anaerobic atmosphere using the GENbag anaer (bioMérieux, Marcy l’Etoile, France). The bacterial identification was performed using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) (Microflex™; Bruker Daltonic, Bremen, Germany) as previously described [ 63 ] and confirmed by 16S rRNA sequencing.…”

Section: Methodsmentioning

confidence: 99%

Prevalence and Antimicrobial Resistance of Paeniclostridium sordellii in Hospital Settings

et al. 2021

View full text Add to dashboard Cite

(1) Background: The purpose of this study was to determine the prevalence of clostridia strains in a hospital environment in Algeria and to evaluate their antimicrobial susceptibility to antibiotics and biocides. (2) Methods: Five hundred surface samples were collected from surfaces in the intensive care unit and surgical wards in the University Hospital of Tlemcen, Algeria. Bacterial identification was carried out using MALDI-TOF-MS, and then the minimum inhibitory concentrations (MICs) of various antimicrobial agents were determined by the E-test method. P. sordellii toxins were searched by enzymatic and PCR assays. Seven products intended for daily disinfection in the hospitals were tested against Clostridium spp. spore collections. (3) Results: Among 100 isolates, 90 P. sordellii were identified, and all strains were devoid of lethal and hemorrhagic toxin genes. Beta-lactam, linezolid, vancomycin, tigecycline, rifampicin, and chloramphenicol all proved effective against isolated strains. Among all strains tested, the spores of P. sordellii exhibited remarkable resistance to the tested biocides compared to other Clostridium species. The (chlorine-based 0.6%, 30 min), (glutaraldehyde solution 2.5%, 30 min), and (hydrogen peroxide/peracetic acid 3%, 15 min) products achieved the required reduction in spores. (4) Conclusions: Our hospital’s current cleaning and disinfection methods need to be optimized to effectively remove spores from caregivers’ hands, equipment, and surfaces.

show abstract

“…Even with a working culture, lack of a comprehensive list of bacterial species in currently available commercial kits, which allow for phenotypic differentiation of the species, may further hamper the correct identification of Cl. estertheticum [53].…”

Section: Isolation and Conventional Culturing Of Clostridium Estermentioning

confidence: 99%

“…Most recently, Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) was used to identify Cl. estertheticum isolated from sheep and cattle carcasses at different slaughtering stages, and the results verified using 16S rDNA gene sequencing [53].…”

Section: Molecular and Non-molecular Based Identification Of Clostmentioning

confidence: 99%

See 1 more Smart Citation

Relevant Aspects of Clostridium estertheticum as a Specific Spoilage Organism of Vacuum-Packed Meat

Wambui

Stephan

2019

Microorganisms

View full text Add to dashboard Cite

Clostridium estertheticum is a psychrotolerant, gram-positive, motile, anaerobic, spore-forming, rod-shaped bacteria that causes blown pack spoilage (BPS). Spoilage occurs in vacuum-packed meat without temperature abuse. Having been reported in the last 30 years in several countries, BPS by Cl. estertheticum is a major issue around the world and presents a huge economic impact on the meat industry. Despite being an important spoilage microorganism, studies on Cl. estertheticum are challenged by numerous aspects. These include, lack or poor growth in laboratory media, long culturing periods, and unpredictable isolation on the media. These factors hamper the detection of Cl. estertheticum before occurrence of BPS, which further undermines efforts to prevent the occurrence of BPS. Nevertheless, considerable developments have taken place with regard to culture-independent methods. Although information on Cl. estertheticum is available, it is limited and remains highly fragmented. Therefore, this review collates the available information and discusses relevant aspects of Cl. estertheticum as a specific spoilage organism of BPS in vacuum-packed meat.

show abstract

Identification of Clostridium spp. derived from a sheep and cattle slaughterhouse by matrix-assisted laser desorption and ionization-time of flight mass spectrometry (MALDI-TOF MS) and 16S rDNA sequencing

Cited by 10 publications

References 46 publications

Determination of Endospore Structure and Volatile Compounds of Clostridial Species During Sporulation

Determination of Endospore Structure and Volatile Compounds of Clostridial Species During Sporulation

Prevalence and Antimicrobial Resistance of Paeniclostridium sordellii in Hospital Settings

Relevant Aspects of Clostridium estertheticum as a Specific Spoilage Organism of Vacuum-Packed Meat

Contact Info

Product

Resources

About