Identification of coagulase‐negative staphylococci from farm animals

Devriese, Luc; Schleifer, Karl‐Heinz; Adegoke, G. O.

doi:10.1111/j.1365-2672.1985.tb01428.x

Cited by 126 publications

(82 citation statements)

References 29 publications

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“…known staphylococcal taxa according to published identification schemes (Devriese et al, 1985;De la Fuente et al, 1985;Kloos & Schleifer, 1986;Freney et al, 1988;Varaldo et al, 1988;Igimi et al, 1989). The biochemical tests used were those previously described (De Buyser et al, 1989).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of a ribosomal RNA gene probe for the identification of species and subspecies within the genus Staphylococcus

Ml¹,

Morvan²,

Aubert³

et al. 1992

Journal of General Microbiology

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To evaluate a 16s rRNA gene probe for the identification of staphylococcal species and subspecies, we have augmented previous studies involving 12 staphylococcal species by analysing the remaining 16 species currently classified in the genus Staphylococcus. HindIII-and EcoRI-restricted DNA of isolates from validly described species of Staphylococcus was probed with radiolabelled plasmid pBA2 containing 16s rDNA from Bacillus subtilis. The Dice coefficient was used to assess similarity between the 74 HindIII-and the 81 EcoRI-hybridization patterns obtained from a total of 271 isolates belonging to 31 staphylococcal taxa (28 species, of which three include two subspecies). The use of HimlIII yielded a better discrimination of the stagbylococci than the use of EcoRI. All of the isolates belonging to the same species or subspecies, except S. hyicus isolates, were recovered as homogeneous clusters using their Hind111 hybridization patterns. The phenotypically close taxa were clearly distinguished. Thus, the method presented in this study constitutes a powerful tool for the identification of taxa within the genus StaphyfococcuA

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Section: Methodsmentioning

confidence: 99%

Evaluation of a ribosomal RNA gene probe for the identification of species and subspecies within the genus Staphylococcus

Ml¹,

Morvan²,

Aubert³

et al. 1992

Journal of General Microbiology

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“…The organization of Staphylococcus spp. into biotypes (2) and further studies performed in the 1970s and 1980s led to the introduction of new species and subspecies of coagulase-negative staphylococci (CoNS) (3)(4)(5)(6)(7).…”

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confidence: 99%

Identification of Coagulase-Negative Staphylococci from Bovine Intramammary Infection by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

et al. 2014

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bCoagulase-negative staphylococci (CoNS) are among the main pathogens causing bovine intramammary infection (IMI) in many countries. However, one of the limitations related to the specific diagnosis of CoNS is the lack of an accurate, rapid, and convenient method that can differentiate the bacterial species comprising this group. The aim of this study was to evaluate the ability of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) to accurately identify CoNS species in dairy cow IMI. In addition, the study aimed to determine the frequency of CoNS species causing bovine IMI. A total of 108 bacterial isolates were diagnosed as CoNS by microbiological cultures from two milk samples collected from 21 dairy herds; the first sample was collected at the cow level (i.e., 1,242 composite samples from all quarters), while the second sample was collected at the mammary quarter level (i.e., 1,140 mammary samples collected from 285 cows). After CoNS isolation was confirmed by microbiological culture for both samples, all CoNS isolates (n ‫؍‬ 108) were genotypically differentiated by PCR restriction fragment length polymorphism (RFLP) analysis of a partial groEL gene sequence and subjected to the MALDI-TOF MS identification procedure. MALDI-TOF MS correctly identified 103 (95.4%) of the CoNS isolates identified by PCR-RFLP at the species level. Eleven CoNS species isolated from bovine IMI were identified by PCR-RFLP, and the most prevalent species was Staphylococcus chromogenes (n ‫؍‬ 80; 74.1%). In conclusion, MALDI-TOF MS may be a reliable alternative method for differentiating CoNS species causing bovine IMI.

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“…Members of the Staphylococcus sciuri group, S. sciuri, S. lentus, S. vitulinus, and S. pulvereri (15), are principally animal species (3,11,17,21), but they may colonize humans, and their isolation from various human clinical specimens has been reported (2,11,13,16,17,22). Moreover, S. sciuri has been associated with serious infections in humans, such as endocarditis (8), peritonitis (24), septic shock (10), and wound infections (20).…”

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confidence: 99%

Isolation of Members of the Staphylococcus sciuri Group from Urine and Their Relationship to Urinary Tract Infections

Stepanović

Ježek²,

Vuković

et al. 2003

J Clin Microbiol

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During a 3-year study period, 32,741 urine samples were analyzed for the presence of members of the Staphylococcus sciuri group (S. sciuri, S. lentus, and S. vitulinus), and 13 isolates were identified. They presented 0.79% of the total number of coagulase-negative staphylococci isolated. One case of symptomatic urinary tract infection and five possible cases of asymptomatic bacteriuria caused by these bacteria were established. It is noteworthy, however, that over 50% of the isolates originated from hospitalized patients.Coagulase-negative staphylococci (CoNS), with the exception of Staphylococcus saprophyticus, are generally considered to be bacteria of doubtful pathogenicity in the urinary tract (1,14). However, a few recent studies proposed that significance of some CoNS species as uropathogens may have been somewhat underestimated (6, 7).Members of the Staphylococcus sciuri group, S. sciuri, S. lentus, S. vitulinus, and S. pulvereri (15), are principally animal species (3,11,17,21), but they may colonize humans, and their isolation from various human clinical specimens has been reported (2,11,13,16,17,22). Moreover, S. sciuri has been associated with serious infections in humans, such as endocarditis (8), peritonitis (24), septic shock (10), and wound infections (20). Several investigators have reported isolation of S. sciuri (4, 6, 16) and S. lentus (6) from urine. However, they did not specify whether the strains isolated were recovered in pure or mixed culture, the colony counts were not reported, and the relationship between the isolates obtained from urine and urinary tract infections (UTIs) was not discussed. The objectives of the present study were to determine the frequency of isolation of the members of S. sciuri group from urine and to evaluate the significance of these isolates as potential ethiological agents of UTIs.Study population. Urine samples were collected from 32,741 hospitalized patients as well as outpatients examined at three hospitals in the region of Příbram The medical records of patients with urine cultures positive for the members of the S. sciuri group were reviewed for symptoms and signs of UTI (dysuria, urinary frequency, urinary urgency, and costovertebral tenderness), underlying medical disorders, and antimicrobial treatment. S. sciuri group isolation and identification.Approximately 95% of samples were cultured on a Uricult dipslide (Orion Diagnostica, Helsinki, Finland), and the remaining specimens were inoculated on Columbia agar supplemented with 5% sheep blood and MacConkey agar. The plates were examined after overnight incubation at 35°C. Any colony that resembled staphylococci was subcultured and further tested. Preliminary identification of an isolate as a member of the S. sciuri group was based upon microscopical characteristics, positive catalase reaction, positive oxidase test, and resistance to novobiocin. Identification to the species level was done with a STAPHYtest 16 kit (Pliva-Lachema, Brno, Czech Republic) and additional biochemical characterization accordi...

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Identification of coagulase‐negative staphylococci from farm animals

Cited by 126 publications

References 29 publications

Evaluation of a ribosomal RNA gene probe for the identification of species and subspecies within the genus Staphylococcus

Evaluation of a ribosomal RNA gene probe for the identification of species and subspecies within the genus Staphylococcus

Identification of Coagulase-Negative Staphylococci from Bovine Intramammary Infection by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Isolation of Members of the Staphylococcus sciuri Group from Urine and Their Relationship to Urinary Tract Infections

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