Identification of Coal Tar Dyes by Reversed-Phase TLC/FABMS with a Sample Condensation Technique

Ozeki, Naoko; Oka, Hisao; Ikai, Yoshitomo; Ohno, Tsutomu; Hayakawa, Junko; Sato, Takeo; Ito, Motome; Suzuki, Ryoji

doi:10.3358/shokueishi.34.512

Cited by 6 publications

(3 citation statements)

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“…Currently, liquid chromatography (LC) coupled with electrospray ionization mass spectrometry (ESI-MS) has been successfully employed to analyze another Isatis species plant I. tinctoria [17]. For the detection of indigo and indirubin, the most common methodologies are TLC, HPLC-UV [18][19][20], and fast atom bombardment mass spectrometry (FAB-MS) [21]. Szostek et al and Puchalska et al recently used LC-MS to investigate the natural dyes (including indigo and indirubin) in Coptic textiles and historical art objects [22,23], but the matrix effect is quite different from that in TCMs.…”

Section: Introductionmentioning

confidence: 99%

LC-APCI-MS method for detection and analysis of tryptanthrin, indigo, and indirubin in Daqingye and Banlangen

Liau

Jong

Lee

et al. 2007

Journal of Pharmaceutical and Biomedical Analysis

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A rapid, selective, and sensitive LC-APCI-MS method is developed in this study for detecting and analyzing tryptanthrin, indigo, and indirubin in daqingye and banlangen, which are, respectively, the leaves and roots of Isatis indigotica and Strobilanthes cusia in traditional Chinese medicine. The detection of the three active components is linear in concentrations ranging from 100 to 1500 ng/mL, the squared correlation coefficient is higher than 0.996, the precision as measured by the relative standard deviation is no larger than 9.5%, and the recovery is greater than 86.6%. The analysis of the 21 banlangen samples led to considerably different conclusions on the contents of tryptanthrin, indigo, and indirubin in fresh leaves versus those in dried leaves. These results should shed some light on future plant selection and breeding. Compared with the traditional TLC and HPLC-UV methods, the new LC-APCI-MS approach has proven to be an optimal tool for detecting and analyzing the three marker compounds in the Chinese herbal medicines of daqingye and banlangen.

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Section: Introductionmentioning

confidence: 99%

LC-APCI-MS method for detection and analysis of tryptanthrin, indigo, and indirubin in Daqingye and Banlangen

Liau

Jong

Lee

et al. 2007

Journal of Pharmaceutical and Biomedical Analysis

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“…For the detection of indirubin and indigo, TLC, HPLC-UV [21], and fast atom bombardment mass spectrometry methods [22] have been described. Puchalska et al [1] and Szostek et al [23] recently used LC-MS to determine and to investigate the natural dyes (including indirubin) in textiles and paintings.…”

Section: Introductionmentioning

confidence: 99%

Quantitative and selective assay of 5-methylindirubine, an inhibitor of cyclin-dependent kinases, in murine plasma using coupled liquid chromatography and electrospray tandem mass spectrometry

Vainchtein

Rosing

Maier³

et al. 2007

Journal of Chromatography B

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“…In particular, reversed-phase TLC, which shows good reproducibility for Rf value, is utilized for routine test of coal tar dyes in foods. [8][9][10][11][12] However, the information obtained from TLC only demonstrates color tone and Rf value. It is very dangerous to identify the coal tar dyes by these factors.…”

Section: Introductionmentioning

confidence: 99%

Identification of Oil-Soluble Coal Tar Dyes in Cosmetics Using Reversed-Phase TLC/Scanning Densitometry

Ohno

Mikami

Matsumoto

2003

JOURNAL OF HEALTH SCIENCE

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A simple and rapid analytical method of oil-soluble coal tar dyes in cosmetics was established using reversed-phase TLC/scanning densitometry. Eleven kinds of oil-soluble coal tar dyes were able to be separated completely on reversed-phase TLC plates by the complementary use of 2 solvent systems. The solvent systems were A; n-hexane/2-butanone solution (5 : 1, v/v), solvent system B; acetonitrile/methanol solution (5 : 1, v/v). Then we measured visible absorption spectra of spots developed on the reversed-phase TLC plates by scanning densitometer to identify these coal tar dyes. The proposed method was successfully applied to the identification of oil-soluble coal tar dyes in commercial cosmetics.

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Identification of Coal Tar Dyes by Reversed-Phase TLC/FABMS with a Sample Condensation Technique

Cited by 6 publications

References 0 publications

LC-APCI-MS method for detection and analysis of tryptanthrin, indigo, and indirubin in Daqingye and Banlangen

LC-APCI-MS method for detection and analysis of tryptanthrin, indigo, and indirubin in Daqingye and Banlangen

Quantitative and selective assay of 5-methylindirubine, an inhibitor of cyclin-dependent kinases, in murine plasma using coupled liquid chromatography and electrospray tandem mass spectrometry

Identification of Oil-Soluble Coal Tar Dyes in Cosmetics Using Reversed-Phase TLC/Scanning Densitometry

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