Identification of Colletotrichum acutatum from rubber using random amplified polymorphic DNAs and ribosomal DNA polymorphisms

Saha, Tushar Kanti; Kumar, Arun; Ravindran, Minimol; Jacob, C. K.; Roy, Bindu; Nazeer, M. A.

doi:10.1017/s0953756201005342

Cited by 28 publications

(33 citation statements)

References 20 publications

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“…26% similarity to the first and second groups. Saha et al (2002), through the restriction of fragments from the ITS region, evalu-(2002), through the restriction of fragments from the ITS region, evaluated the Colletotrichum species that caused lesions in Hevea brasiliensis. It was possible to observe the grouping of species that caused larger lesions.…”

Section: Resultsmentioning

confidence: 99%

Pathogenicity for onion and genetic diversity of isolates of the pathogenic fungus Colletotrichum gloeosporioides (Phyllachoraceae) from the State of Pernambuco, Brazil

Mx¹,

Lr²,

Ac³

et al. 2011

Genet. Mol. Res.

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ABSTRACT. Onion anthracnose, caused by Colletotrichum gloeosporioides, is one of the main diseases of onions in the State of Pernambuco. We examined the pathogenicity of 15 C. gloeosporioides strains and analyzed their genetic variability using RAPDs and internal transcribed spacers (ITS) of the rDNA region. Ten of the strains were obtained from substrates and hosts other than onion, including chayote (Sechium edule), guava (Psidium guajava), pomegranate (Punica granatum), water from the Capibaribe River, maracock (Passiflora sp), coconut (Cocus nucifera), surinam cherry (Eugenia uniflora), and marine soil; five isolates came from onions collected from four different regions of the State of Pernambuco and one region of the State of Amazonas. Pathogenicity tests were carried out using onion leaves and bulbs. All strains were capable of causing disease in leaves, causing a variable degree of lesions on the leaves; four strains caused the most severe damage. In the onion bulb tests, only three of the above strains caused lesions. Seven primers of arbitrary sequences were used in the RAPD analysis, generating polymorphic bands that allowed the separation of the strains into three distinct groups. The amplification products generated with the primers ITS1 and ITS4 also showed polymorphism when digested with three restriction enzymes, DraI, HaeIII and MspI. Only the latter two demonstrated genetic variations among the strains. These two types of molecular markers were able to differentiate the strain from the State of Amazonas from those of the State of Pernambuco. However, there was no relationship between groups of strains, based on molecular markers, and degree of pathogenicity for onion leaves and bulbs.

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Section: Resultsmentioning

confidence: 99%

Pathogenicity for onion and genetic diversity of isolates of the pathogenic fungus Colletotrichum gloeosporioides (Phyllachoraceae) from the State of Pernambuco, Brazil

Mx¹,

Lr²,

Ac³

et al. 2011

Genet. Mol. Res.

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“…Among these molecular techniques, DNA fragment analysis [e.g. randomly amplified polymorphic DNA (RAPD), and arbitrarily primed (AP)-PCR] has been extensively used to investigate relationships among isolates of many fungal genera including Colletotrichum spp [8,9]. Similarly, nucleotide sequence information for the 5.8S gene and the internal transcribed spacer [ITS] region of ribosomal DNA (rDNA) has been used to design Colletotrichum species-specific primers for diagnostic purposes and for phylogenetic analysis [5,10].…”

Section: Introductionmentioning

confidence: 99%

PCR-Based Detection and Characterization of the Fungal Pathogens Colletotrichum gloeosporioides and Colletotrichum capsici Causing Anthracnose in Papaya (Carica papaya L.) in the Yucatan Peninsula

et al. 2008

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Colletotrichum gloeosporioides is the common causal agent of anthracnose in papaya (Carica papaya L.) fruits, and infection by this fungal pathogen results in severe post-harvest losses. In the Yucatán peninsula (Mexico) a different Colletotrichum species was isolated from papaya fruits with atypical anthracnose lesions. The DNAs from a variety of Colletotrichum isolates producing typical and atypical lesions, respectively, were amplified by PCR with C.gloeosporioides-specific primers. All isolates from typical anthracnose lesions yielded a 450 bp PCR product, but DNAs from isolates with atypical lesions failed to produce an amplification product. For further characterization, the rDNA 5.8S-ITS region was amplified by PCR and processed for sequencing and RFLP analysis, respectively, to verify the identity of the papaya anthracnose pathogens. The results revealed unequivocally the existence of two Colletotrichum species causing anthracnose lesions on papaya fruits: C. gloeosporioides and C. capsici. PCR-RFLP using the restriction endonuclease MspI reliably reproduced restriction patterns specific for C. capsici or C. gloeosporioides. The generation of RFLP patterns by MspI (or AluI or RsaI) is a rapid, accurate, and unequivocal method for the detection and differentiation of these two Colletotrichum species.

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“…2A). Several authors working with primers ITS1 and ITS2 observed that the majority of isolates identified as C. gloeosporioides, showed a single fragment of approximately 590-600 bp (Abang, 2002;Martínez-Culebraz et al, 2000;Saha, 2002). The digestion of the fragments of the ITS with DraI produced a monomorphic fragment of approximately 400-bp, for all Colletotrichum sp.…”

Section: Polymorphism Of Fragments Digestion Of the Its Rdna Regions mentioning

confidence: 99%

[No Title Available]

Maciel

Medeiros

et al. 2010

Braz. arch. biol. technol.

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Identification of Colletotrichum acutatum from rubber using random amplified polymorphic DNAs and ribosomal DNA polymorphisms

Cited by 28 publications

References 20 publications

Pathogenicity for onion and genetic diversity of isolates of the pathogenic fungus Colletotrichum gloeosporioides (Phyllachoraceae) from the State of Pernambuco, Brazil

Pathogenicity for onion and genetic diversity of isolates of the pathogenic fungus Colletotrichum gloeosporioides (Phyllachoraceae) from the State of Pernambuco, Brazil

PCR-Based Detection and Characterization of the Fungal Pathogens Colletotrichum gloeosporioides and Colletotrichum capsici Causing Anthracnose in Papaya (Carica papaya L.) in the Yucatan Peninsula

[No Title Available]

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