Identification of compounds from Palicourea rigida leaves with topical anti-inflammatory potential using experimental models

Abstract

Palicourea rigida Kunth is traditionally used for the treatment of skin diseases, kidney pains and ovarian inflammation. Based on these traditional uses, this study evaluated the topical anti-inflammatory activity of the ethanol extract from P. rigida leaves (EEPR) and identified bioactive compounds. Ear edema was induced in Swiss mice by the topical application of Croton oil, arachidonic acid, phenol and capsaicin. Histopathological analysis and myeloperoxidase and N-acetyl-β-D-glucosaminidase activities were determined. EEPR was characterized by UHPLC-UV-MS HPLC and the isolated compound was identified through H andC nuclear magnetic resonance and mass fragmentation. Interaction profiles between quercetin 3-O-β-D-glucoside and cyclooxygenase-1 and -2 were established by molecular docking. EEPR significantly inhibited ear edema induced by Croton oil (p < 0.001), arachidonic acid (p < 0.01), phenol (p < 0.001) and capsaicin (p < 0.01 or p < 0.001). Histopathological analysis showed a reduction of edema, inflammatory cell infiltration and vasodilation. Additionally, the myeloperoxidase and N-acetyl-β-D-glucosaminidase activities were decreased (p < 0.001). From spectroscopic data, quercetin 3-O-β-D-glucoside was the identified compound. This compound can to interact with cyclooxygenase-1 and -2 through van der Waals interactions and dipole-dipole and hydrogen bonding's, demonstrating inhibition of these enzymes. The results indicate that EEPR is a source of active compounds with topical anti-inflammatory activity, justifying the traditional use of P. rigida and showing that this species has a therapeutic potential to treat skin inflammatory processes.

Antileukemic, Antioxidant, Anti-Inflammatory and Healing Activities Induced by a Polyphenol-Enriched Fraction Extracted from Leaves of Myrtus communis L.

“…Although only a few studies of the pharmacological potential of the isorhamnetin-7-O-pentose have been reported, the flavonoid shows a great therapeutic potential for type 2 diabetes by down-regulating the protein tyrosine phosphatase-1B (PTP1B) expression [ 65 ]. The quercetin-3-O-glucoside demonstrated anti-inflammatory and antileukemic activities by inhibiting cyclooxygenase-1/2 pathway and inducing apoptosis, respectively [ 66 , 67 ]. The luteolin 7-O-glucoside is a glycosyloxyflavone that acts as an antioxidant and an anti-inflammatory agent through a regulation of inflammatory mediators and oxidative stress [ 68 , 69 , 70 ].…”

POTENCIAL DE USO DA Averrhoa carambola l. PARA DIABETES

“…A ação anti-inflamatória tópica do extrato etanólico, fração hexânica e do flavonoide 3-O-β-D-glicosídeo foi demonstrada em estudo com a planta P. rigida (Alves, et al, 2016;Pinheiro et al, 2017), além de sua potencial ação em células de melanoma humano (SK Mel 37) atribuído pela presença do alcaloide vallesiachotamina (Silva et al, 2006). Análise fitoquímica e biológica da espécie P. hoffmannseggiana revelou a presença de vários compostos, principalmente alcaloides, naftoquinonas e iridoides os quais demonstraram comprovada atividade antidepressiva, citotóxica, anti-inflamatória e antimalárica (Ohashi & Oliveira 2020).…”

Insights into the Bioprospecting of the Endophytic Fungi of the Medicinal Plant Palicourea rigida Kunth (Rubiaceae): Detailed Biological Activities

“…In this perspective, Palicourea rigida Kunth (Rubiaceae) is a medicinal plant native to the savanna and used in folk medicine for the treatment of urinary disorders and disorders of the female reproductive system [25,26]. It is also used as a diuretic, hypotensive, healer, and treatment for stomach and kidney pain and for coughs [27]. However, the endophytic mycobiota of this species have not been reported yet, including their bioactive properties.…”