Identification of coryneform bacteria and related taxa by Fourier-transform infrared (FT-IR) spectroscopy.

Oberreuter, Helene; Seiler, H.; Scherer, Siegfried

doi:10.1099/00207713-52-1-91

Cited by 128 publications

(113 citation statements)

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“…The reference work of Helm and colleagues (14) prompted a large amount of research, reviewed by Mariey and colleagues (26). Since 2001, more groups and genera have been studied: Lactobacillus (3,36,37), Carnobacterium (21), Bacillus (1), Brucella (27), Acinetobacter (52), yeasts (20,41), enterococci (7,10,19), coryneforms (24,34), and actinomycetes (54). In all of them, the technique has shown qualities, such as high discriminatory power and reproducibility, allowing the differentiation of taxonomic entities at the species or subspecies level on the basis of changes in the IR absorption patterns of the microbial cell.…”

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confidence: 99%

Discrimination of Enterobacterial Repetitive Intergenic Consensus PCR Types of Campylobacter coli and Campylobacter jejuni by Fourier Transform Infrared Spectroscopy

Mouwen

Weijtens

Capita

et al. 2005

Appl Environ Microbiol

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Fourier transform infrared spectroscopy (FT-IR) has been used together with pattern recognition methodology to study isolates belonging to the species Campylobacter coli and Campylobacter jejuni and to compare FT-IR typing schemes with established genomic profiles based on enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). Seventeen isolates were cultivated under standardized conditions for 2, 3, and 4 days to study variability and improve reproducibility. ERIC-PCR profiles and FT-IR spectra were obtained from strains belonging to the species Campylobacter coli and C. jejuni, normalized, and explored by hierarchical clustering and stepwise discriminant analysis. Strains could be differentiated by using mainly the firstderivative FT-IR spectral range, 1,200 to 900 cm ؊1 (described as the carbohydrate region). The reproducibility index varied depending on the ages of the cultures and on the spectral ranges investigated. Classification obtained by FT-IR spectroscopy provided valuable taxonomic information and was mostly in agreement with data from the genotypic method, ERIC-PCR. The classification functions obtained from the discriminant analysis allowed the identification of 98.72% of isolates from the validation set. FT-IR can serve as a valuable tool in the classification, identification, and typing of thermophilic Campylobacter isolates, and a number of types can be differentiated by means of FT-IR spectroscopy.

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confidence: 99%

Discrimination of Enterobacterial Repetitive Intergenic Consensus PCR Types of Campylobacter coli and Campylobacter jejuni by Fourier Transform Infrared Spectroscopy

Mouwen

Weijtens

Capita

et al. 2005

Appl Environ Microbiol

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“…Complete 16S rRNA gene sequences were amplified by PCR (Büchl et al, 2008) using universal primers and cycling conditions specified by Oberreuter et al (2002). Amplification products were purified with QIAquick PCR Purification Kit (Qiagen) and cycle sequencing PCR was performed by Sequiserve (Vaterstetten, Germany).…”

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confidence: 99%

“…To reduce the difficulties arising from unavoidable baseline shifts and to improve the resolution of complex bands, the first derivation of the digitized original spectra was used for data evaluation. Isolates were identified using spectral reference libraries of coryneform bacteria (Oberreuter et al, 2002) and lactic acid bacteria (M. Wenning and others, unpublished data). As expected, the majority of bacteria forming the consortia belonged to the class Actinobacteria.…”

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confidence: 99%

Bavariicoccus seileri gen. nov., sp. nov., isolated from the surface and smear water of German red smear soft cheese

Schmidt

Mayr

Wenning

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Bavariicoccus seileri gen. nov., sp. nov., isolated from the surface and smear water of German red smear soft cheese The phylogenetic position and physiological characters of six hitherto-unknown lactic acid bacterial isolates, which form part of the surface microbiota of German red smear soft cheese, are reported. The coccoid cells are aerotolerant, Gram-positive, catalase-negative and non-motile. The cell-wall peptidoglycan contains alanine, glutamic acid, lysine and aspartic acid and is of the A4a type (L-Lys-D-Asp). The sequences of the 16S rRNA, groEL and rpoB genes of the six isolates are identical and reveal that these isolates represent an independent lineage within the radiation of the family Enterococcaceae in the phylum Firmicutes. Their closest phylogenetic neighbour is the lactic acid bacterium Atopobacter phocae M1590/94/2 T , with which they share 94.9 % 16S rRNA gene sequence similarity; representatives of other genera such as Granulicatella, Carnobacterium and Trichococcus are more distantly related. DNA-DNA hybridization studies reveal that the six isolates are members of a single species, and this is confirmed by similarities in biochemical characteristics. The six isolates were assigned four different groups by Fourier-transform infrared and randomly amplified polymorphic DNA typing. Therefore, it is formally proposed that these isolates should be classified in a single novel species of a novel genus and be named Bavariicoccus seileri gen. nov., sp. nov. The type strain of Bavariicoccus seileri is WCC 4188 T (5DSM 19936 T 5CCUG 55508 T ).Red smear cheeses are mainly produced in Austria, Germany, France, Scandinavia and Switzerland. For the ripening process, the microbial consortia of mature cheeses are washed off the surfaces with a brine solution, which is then used for inoculation of freshly produced cheeses. By virtue of the traditional manufacturing process, a variety of different smear cheeses exist which often comprise quite complex, species-rich microbial consortia (Brennan et al., 2004;Feurer et al., 2004;Maoz et al., 2003; Mounier et al., 2005;Wenning et al., 2006). Some recently described species have been isolated from these consortia, such as We have studied the species composition of South German red smear microbial consortia by aerobic cultivation on PC agar supplemented with 3 % NaCl (Maoz et al., 2003) and quantitative analysis of the species composition by Fouriertransform infrared (FT-IR) spectroscopy. FT-IR sample preparation, spectrum recording and data evaluation were performed as described by Kümmerle et al. (1998) using an IFS-28B spectrometer and OPUS version 5.5 (Bruker). To reduce the difficulties arising from unavoidable baseline shifts and to improve the resolution of complex bands, the first derivation of the digitized original spectra was used for data evaluation. Isolates were identified using spectral Abbreviation: FT-IR, Fourier-transform infrared; RAPD, randomly amplified polymorphic DNA. 3Present address:

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“…Antibiotic susceptibilities were determined by the disk diffusion method on Mueller-Hinton medium (Difco Laboratories), according to the methods outlined by the Clinical and Laboratory Standards Institute, formerly the National Committee for Clinical Laboratory Standard [25]. The antibiotics used are: ampicillin 10 μg teicoplanin 30 μg and vancomycin 30 μg all from oxoid limited England.…”

Section: Antibiotics Susceptibility Testingmentioning

confidence: 99%

Fourier Transform Infrared Spectroscopy Typing an <i>Enterococcus</i> sp.

Taha¹,

Eideh²,

Saed³

et al. 2014

JMP

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Enterococcus species are one of the leading causes of nosocomial infections, which are difficult to treat specially with the rise of its Vancomycin resistant. Studies of Enterococcus isolates are essential for epidemiological investigation. Typing Enterococci is often based on the traditional phenotypic as well as genotypic methods. In this study Fourier-transform infrared (FTIR) spectroscopy is used as a novel phenotypic approach to the typing of Enterococci.

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Identification of coryneform bacteria and related taxa by Fourier-transform infrared (FT-IR) spectroscopy.

Cited by 128 publications

References 45 publications

Discrimination of Enterobacterial Repetitive Intergenic Consensus PCR Types of Campylobacter coli and Campylobacter jejuni by Fourier Transform Infrared Spectroscopy

Discrimination of Enterobacterial Repetitive Intergenic Consensus PCR Types of Campylobacter coli and Campylobacter jejuni by Fourier Transform Infrared Spectroscopy

Bavariicoccus seileri gen. nov., sp. nov., isolated from the surface and smear water of German red smear soft cheese

Fourier Transform Infrared Spectroscopy Typing an <i>Enterococcus</i> sp.

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Identification of coryneform bacteria and related taxa by Fourier-transform infrared (FT-IR) spectroscopy.

Cited by 128 publications

References 45 publications

Discrimination of Enterobacterial Repetitive Intergenic Consensus PCR Types of Campylobacter coli and Campylobacter jejuni by Fourier Transform Infrared Spectroscopy

Discrimination of Enterobacterial Repetitive Intergenic Consensus PCR Types of Campylobacter coli and Campylobacter jejuni by Fourier Transform Infrared Spectroscopy

Bavariicoccus seileri gen. nov., sp. nov., isolated from the surface and smear water of German red smear soft cheese

Fourier Transform Infrared Spectroscopy Typing an &lt;i&gt;Enterococcus&lt;/i&gt; sp.

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Fourier Transform Infrared Spectroscopy Typing an <i>Enterococcus</i> sp.