Identification of Crude Drugs from Chinese Medicinal Plants of the Genus<i>Bupleurum</i>Using Ribosomal DNA ITS Sequences

Xie, Hui; Huo, Keke; Chao, Zhi; Pan, Sheng-Li

doi:10.1055/s-0028-1088334

Cited by 29 publications

(13 citation statements)

References 7 publications

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“…Preparations containing the roots of Bupleurum species have been prescribed for more than 2000 years in China where the first record about their use appeared in ShenNong's Herbal. [11] Inspired by the role in regulating metabolism and controlling Yin/Yang as mentioned in the old Chinese literature, Bupleurum was widely known in Korea and Japan for the treatment of fever, pain and inflammation associated with influenza and the common cold. [4,10] In addition, Bupleurum species were also used as analgesics in the treatment of distending pain in the hypochondriac region of the chest and against amenorrhoea.…”

Section: Introductionmentioning

confidence: 99%

Genus Bupleurum: a review of its phytochemistry, pharmacology and modes of action

Ashour

Wink

2011

Journal of Pharmacy and Pharmacology

229

161

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Objectives Radix Bupleuri represents one of the most successful and widely used herbal drugs in Asia for treatment of many diseases over the past 2000 years. Thorough studies have been carried out on many species of this genus and have generated immense data about the chemical composition and corresponding biological activity of extracts and isolated secondary metabolites. In this work, we review the chemistry and pharmacology of the genus Bupleurum and explore the relationships between the pharmacological effects and the chemical composition of these drugs. Key findings Early studies on the genus Bupleurum had focused only on the traditional uses of the plants in the treatment of inflammatory disorders and infectious diseases. After chemical profiling, several groups of secondary metabolites were characterized with relevant biological activity: triterpene saponins (saikosaponins), lignans, essential oils and polysaccharides. As a result, present interest is now focused on the bioactivity of the isolated triterpene saponins acting as immunomodulatory, anti-inflammatory and antiviral agents, as well as on the observed ant-iulcer activity of the polysaccharides and anti-proliferative activity of different lignans. Many saikosaponins exhibited very potent anti-inflammatory, hepatoprotective and immunomodulatory activities both in vivo and in vitro. Conclusions Further investigations and screenings are required to explore other Bupleurum species, to evaluate the clinical safety and possible interactions with other drugs or herbs. Standardization of Bupleuri extracts is crucial for them being integrated into conventional medicine due to large chemical and biological variations between different species and varieties.

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Section: Introductionmentioning

confidence: 99%

Genus Bupleurum: a review of its phytochemistry, pharmacology and modes of action

Ashour

Wink

2011

Journal of Pharmacy and Pharmacology

229

161

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“…To identify medicinal plants or to discriminate medicinal plants from other plants belonging to the same genus, ITS sequences and/or the difference in these sequences are commonly and efficiently utilized. Determination of ITS sequences and analysis of their variation have been applied to identify medicinal plants originated from the genera Bupleurum (Xie et al, 2009), Atractylodes (Shiba et al, 2006), and Phyllanthus Nucleotide position 23 36 46 53 54 70 79 88 96 147 155 173 180 188 196 S. ningpoensis (Lee et al, 2006). Presently, we determined and analyzed ITS sequences of S. ningpoensis and S. buergeriana, as well as S. koraiensis, which is occasionally prescribed in lieu of S. buergeriana in Korea.…”

Section: Discussionmentioning

confidence: 98%

“…To overcome the problem of these methods, various molecular biology techniques utilizing different genetic information of organism, are used for the discrimination of plant species (McClelland and Welsh, 1994). In particular, the analysis of internal transcribed sequence (ITS) has been used for this purpose (Lee et al, 2006;Shiba et al, 2006;Xie et al, 2009). Presently, we developed reliable DNA markers based on the ITS sequence to discriminate S. buergeriana and S. ningpoensis.…”

Section: Introductionmentioning

confidence: 99%

Discrimination of three Scrophularia plants utilizing ‘Scrophularia Radix’ by DNA markers based on internal transcribed spacer (ITS) sequences

et al. 2010

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“…이러한 ITS 염기서열은 약용식물의 감별을 위해서도 효율적으로 사용되고 있다 27) . 예를 들어, Atractylodes 속 28) , Bupleurum 속 29) , Phyllanthus 속 …”

Section: 목향 감별 Dna 마커 제작unclassified

Identification 4 kinds of Muxiang using Multiplex PCR

Doh¹,

Lee²,

Ju³

et al. 2014

The Korea Journal of Herbology

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Objectives : Aucklandiae Radix (Muxiang) one of important herbal medicines in oriental medicine, is defined as the dried root of Aucklandia lappa (Asteraceae). Owing to the similarities in the morphology and name, Inulae Radix (Tu-Muxiang) and Vladimiriae Radix (Chuan-Muxiang) as well as Aristolochiae Radix (Qing-Muxiang) originated from other medicinal plants are often used as substitutes and/or adulterants of Aucklandiae Radix. Therefore, a reliable authentication of these herbal medicines is necessarily for the public health and prevention of misuse. Methods : 32 samples of medicinal plants supplying Aucklandiae Radix, Inulae Radix, Vladimiriae Radix, and Aristolochiae Radix were collected in Korea and China. The ITS (Internal transcribed spacer) nucleotide sequences of samples were determined. The PCR primers to amply DNA marker of each herbal medicine were designed basing on the specific ITS regions showing differences in the sequences among medicinal plants. Results : Primer set Al R/IS F designed in this work amplified 220 bp PCR product only in samples of Aucklandiae Radix. In contrast, primer set Ih F/IS R, Vs R/IS F, and AcR F1/Ac R amplified 250 bp product, 356 bp prouct, and 516 bp product respectively to identify Inulae Radix, Vladimiriae Radix, and Aristolochiae Radix. Conclusions : The primers designed basing on the nucleotide sequences of ITS regions appearing differenced in the sequences among medicinal plants amplified the DNA markers for the identification of Aucklandiae Radix, Inulae Radix, Vladimiriae Radix, and Aristolochiae Radix. These herbal medicines were more efficiently identified by multiplex PCR method using all primers in a single PCR process.

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Identification of Crude Drugs from Chinese Medicinal Plants of the GenusBupleurumUsing Ribosomal DNA ITS Sequences

Cited by 29 publications

References 7 publications

Genus Bupleurum: a review of its phytochemistry, pharmacology and modes of action

Genus Bupleurum: a review of its phytochemistry, pharmacology and modes of action

Discrimination of three Scrophularia plants utilizing ‘Scrophularia Radix’ by DNA markers based on internal transcribed spacer (ITS) sequences

Identification 4 kinds of Muxiang using Multiplex PCR

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