Identification of CTX-M-14 Extended-Spectrum β-Lactamase in Clinical Isolates of
 Shigella sonnei
 ,
 Escherichia coli
 , and
 Klebsiella pneumoniae
 in Korea

Pai, Hyunjoo; Choi, Eunhwa; Lee, Hoan-Jong; Hong, Jeum Kyu; Jacoby, George A.

doi:10.1128/jcm.39.10.3747-3749.2001

Cited by 126 publications

(103 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This observation is in good agreement with the previous publication of Bradford et al (10) reporting the location of genes encoding CTX-M-5 on small non-self-transferable plasmids. It may also explain the relatively low proportion of CTX-M-5-producing isolates compared to those bearing CTX-M-3 and Neither CTX-M-8-nor CTX-M-9-related enzymes were found in this study, although in recent years ESBLs of the latter genetic group were found in other European countries (Spain [9,11,35], France [18,36], and the United Kingdom [Alobwede et al, letter]) as well as in Asian countries (Japan [41], China [14], Taiwan [22,44], Korea [28], and Vietnam [13]) and Brazil (8).…”

Section: Resultsmentioning

confidence: 63%

Prevalence and Molecular Epidemiology of CTX-MExtended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in RussianHospitals

Edelstein

Pimkin

Palagin

et al. 2003

Antimicrob Agents Chemother

329

181

View full text Add to dashboard Cite

A total of 904 consecutive nosocomial isolates of Escherichia coli and Klebsiella pneumoniae collected from 28 Russian hospitals were screened for production of extended-spectrum ␤-lactamases (ESBLs). The ESBL phenotype was detected in 78 (15.8%) E. coli and 248 (60.8%) K. pneumoniae isolates. One hundred fifteen isolates carried the genes for CTX-M-type ␤-lactamases, which, as shown by PCR-restriction fragment length polymorphism analysis, were distributed into the two genetic groups of CTX-M-1 (93%)-and CTX-M-2 (7%)-related enzymes. Isolates producing the enzymes of the first group were found in 20 hospitals from geographically distant regions of the country and were characterized by considerable diversity of genetic types, as was demonstrated by enterobacterial repetitive consensus PCR typing. Within this group the CTX-M-3 and the CTX-M-15 ␤-lactamases were identified. In contrast, the enzymes of the CTX-M-2 group (namely, CTX-M-5) were detected only in eight clonally related E. coli isolates from a single hospital. Notably, the levels of resistance to ceftazidime were remarkably variable among the CTX-M producers. This study provides further evidence of the global dissemination of CTX-M type ESBLs and emphasizes the need for their epidemiological monitoring.During the past decade extended-spectrum ␤-lactamases (ESBLs) of the CTX-M type emerged in many countries of the world. The first organisms producing ␤-lactamases of this type were identified both as single and epidemic clinical isolates in very distant geographic regions (Germany and France and Argentina) in the early 1990s (3, 5, 7). More recently, a rapid increase in the proportion of multiple CTX-M variants to the TEM-and SHV-derived ESBLs has been reported in many hospitals in Spain (9,12,15,35) (14), and Korea (28). Furthermore, CTX-M ␤-lactamases, mainly types CTX-M-2 and CTX-M-3, were found to be widespread or even predominant ESBL types in several countries, including Argentina (31, 34; M. Galas, F. Pasteran, R. Melano, A. Petroni, G. Lopez, A. Corso, A. Rossi, et al., Abstr. 38th Intersci. Conf. Antimicrob. Agents Chemother., abstr. E-109, 1998), Japan (41), and Poland (1).Currently the CTX-M family includes more than 20 ␤-lactamases, which may be grouped on the basis of sequence similarity into four distinct clusters (subtypes) epitomized by CTX-M-1, CTX-M-2, CTX-M-8, and CTX-M-9 (26, 37; http: //www.lahey.org/studies/webt.htm). Within each cluster there is a high degree of bla CTX-M gene sequence identity (Ͼ95%), although members of different subtypes share only 70 to 77% similarity at the nucleotide level. The recent finding of Kluyvera ascorbata species-specific ␤-lactamases (KluA) which share 98.6 to 100% identity with CTX-M-2 and CTX-M-5 and analysis of DNA sequences adjacent to the KluA-and CTX-Mcoding genes provide strong evidence of the direct evolution of the CTX-M-2 cluster from the chromosomal enzyme of K. ascorbata (20,36). Likewise, the chromosomal KLUG-1 enzyme of Kluyvera georgiana was found to be the most-probable progenitor of ...

show abstract

Section: Resultsmentioning

confidence: 63%

Prevalence and Molecular Epidemiology of CTX-MExtended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in RussianHospitals

Edelstein

Pimkin

Palagin

et al. 2003

Antimicrob Agents Chemother

329

181

View full text Add to dashboard Cite

show abstract

“…Table 1 lists the bacterial strains used in this study and the ␤-lactamases that they produce. The enzymes produced by previously unpublished strains were identified by isoelectric focusing, PCR amplification, cloning, and sequencing, as described previously (1,19,28,36). Plasmids were transferred to porin-deficient K. pneumoniae strain C2 (24) by conjugation (16).…”

Section: Methodsmentioning

confidence: 99%

Identification of Extended-Spectrum, AmpC, and Carbapenem- Hydrolyzing β-Lactamases in Escherichia coli and Klebsiella pneumoniae by Disk Tests

2006

Self Cite

View full text Add to dashboard Cite

Antibiotic disks with and without clavulanic acid, 3-aminophenylboronic acid, or EDTA were tested with a set of 55 Klebsiella pneumoniae and Escherichia coli strains producing well-characterized extended-spectrum, AmpC, or carbapenem-hydrolyzing β-lactamases. A relatively simple scheme was devised for distinguishing β-lactamase types in clinical isolates with or without intact outer membrane porins.

show abstract

“…국내에서는 처음으로 2001년 CTX-M-14를 생성하는 E. coli와 K. pneumoniae가 보고되었으며 [21], 2003년 전국 13개 병원에서 분리된 E. coli의 9%가 ESBL을 생성한다고 보고하 였다 [23].…”

Section: 서 론unclassified

“…국내에서도 2002년 전국 13개 병원을 대상으로 조사한 ESBL 출현 현황에서 E. coli와 K. pneumoniae 가 CTX-M (CTX-M-3, CTX-M-14, CTX-M-15) 유전자를 보유 하고 있다고 하였다 [2,21].…”

Section: 서 론unclassified

Prevalence of Extended-spectrum β-Lactamase and Quinolone Resistance Genes in Escherichia coli Clinical Isolates and their Antibiotic Resistance

Lee¹,

Hwang²,

Baik³

et al. 2013

Journal of Life Science

View full text Add to dashboard Cite

The aim of this study was to investigate the prevalence of Extended-spectrum β-lactamase (ESBL) gene and quinolone resistance determinant (qnr) and the pattern of antibiotic resistance in the ESBL-producing Escherichia coli clinical isolates. The 42 ESBL-producing strains from total 274 isolates were detected using a double disk synergy test. They were isolated from various specimens, such as urine (28 strains), sputum (6 strains), pus (3 strains), wound (2 strains), blood (2 strains), and tissue (1 strain). Using the PCR with the specific primers ESBL, ESBL and qnr gene types were determined.Thirty-five strains possessed one or two ESBL genes. CTX-M-1 type was the most abundant followed by CTX-M-9 type and TEM, but SHV, CTX-M-2, and CTX-M-8 gene types were not detected. qnr gene types were detected from ten isolates in the order of qnrB4, qnrB1, and qnrS. Coexistence of ESBL and qnr genes was found. ESBL-producing isolates showed high resistance against some antibiotics, such as cefotaxmie (80.0%), levofloxacin (82.9%), and ampicillin (100%). Neither a synergy effect from the coexistence of ESBL and qnr genes on antibiotic resistance nor a correlation between the production of qnr gene and quinolone resistance were found.Key words : Extended-spectrum β-lactamase, quinolone resistance, Escherichia coli, antibiotic resistance *Corresponding author *Tel：+82-61-750-3613, Fax：+82-61-750-5469 *E-mail : scnu@sunchon.ac.kr This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

show abstract

Identification of CTX-M-14 Extended-Spectrum β-Lactamase in Clinical Isolates of Shigella sonnei , Escherichia coli , and Klebsiella pneumoniae in Korea

Cited by 126 publications

References 10 publications

Prevalence and Molecular Epidemiology of CTX-MExtended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in RussianHospitals

Prevalence and Molecular Epidemiology of CTX-MExtended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in RussianHospitals

Identification of Extended-Spectrum, AmpC, and Carbapenem- Hydrolyzing β-Lactamases in Escherichia coli and Klebsiella pneumoniae by Disk Tests

Prevalence of Extended-spectrum β-Lactamase and Quinolone Resistance Genes in Escherichia coli Clinical Isolates and their Antibiotic Resistance

Contact Info

Product

Resources

About