Identification of cucurbit chlorotic yellows virus P4.9 as a possible movement protein

Ying, Wei; Shi, Yong; Han, X. Y.; Chen, Siyu; Li, Honglian; Chen, Linlin; Sun, Bing‐Jian; Shi, Yan

doi:10.1186/s12985-019-1192-y

Cited by 8 publications

(5 citation statements)

References 20 publications

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“…The plasmodesmata (PD) is the critical macromolecule transport corridor in plant cell walls. Because of its size exclusion limit (SEL), PD is a bottleneck during plant virus infections [42]. Many studies have demonstrated that plant virus infections can induce callose accumulation around PDs, leading to a restriction of virus intercellular trafficking.…”

Section: Discussionmentioning

confidence: 99%

Discovery and Genomic Function of a Novel Rice Dwarf-Associated Bunya-like Virus

Wang

et al. 2022

Viruses

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Bunyaviruses cause diseases in vertebrates, arthropods, and plants. Here, we used high-throughput RNA-seq to identify a bunya-like virus in rice plants showing the dwarfing symptom, which was tentatively named rice dwarf-associated bunya-like virus (RDaBV). The RDaBV genome consists of L, M, and S segments. The L segment has 6562 nt, and encodes an RdRp with a conserved Bunya_RdRp super family domain. The M segment has 1667 nt and encodes a nonstructural protein (NS). The complementary strand of the 1120 nt S segment encodes a nucleocapsid protein (N), while its viral strand encodes a small nonstructural protein (NSs). The amino acid (aa) sequence identities of RdRp, NS, and N between RDaBV and viruses from the family Discoviridae were the highest. Surprisingly, the RDaBV NSs protein did not match any viral proteins. Phylogenetic analysis based on RdRp indicated that RDaBV is evolutionarily close to viruses in the family Discoviridae. The PVX-expressed system indicated that RDaBV N and NS may be symptom determinants of RDaBV. Our movement complementation and callose staining experiment results confirmed that RDaBV NSs is a viral movement protein in plants, while an agro-infiltration experiment found that RDaBV NS is an RNA silencing suppressor. Thus, we determined that RDaBV is a novel rice-infecting bunya-like virus.

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Section: Discussionmentioning

confidence: 99%

Discovery and Genomic Function of a Novel Rice Dwarf-Associated Bunya-like Virus

Wang

et al. 2022

Viruses

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“…The changed adenine and guanine have been proved that utilizing for RNA synthesis. CCYV is a single strain RNA virus with a bipartite genome [ 42 ]. Our recent work proved that the titers of CCYV within host plant increased from 10 to 30 dpi [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

Widely targeted analysis of metabolomic changes of Cucumis sativus induced by cucurbit chlorotic yellows virus

Zhang

Yan

et al. 2022

BMC Plant Biol

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Background Plant metabolites play vital roles in regulating the behavior of herbivore insects. Virus infection can universally alter plant metabolites to manipulate the orientation and feeding behaviors of insect vector, to favor the transmission of virus. Thus, determining the differentially accumulated metabolites of plant upon virus infection could provide insights into understanding how the triple interactions among plant, virus and insect vector happens. Our previous studies have found that vector whitefly Bemisia tabaci (Gennadius, Hemiptera: Aleyrodidae) showed different orientation behavior and performance on CCYV-infected and healthy cucumber plants. Cucurbit chlorotic yellows virus (CCYV) is exclusively transmitted by B. tabaci in a semi-persistent mode. In this study, we take the CCYV, B. tabaci and cucumber as a research system to explore the functions of phyto-metabolites in the triple interactions. Results A total of 612 metabolites changed upon CCYV infection were monitored. Metabolites mainly enriched in flavonoids, lipids, nucleotides and their derivatives. At 7 days post CCYV inoculation (dpi), the contents of lipids, terpenoids and flavonoids remarkably decreased, while amino acids, nucleotides and their derivatives notably up-accumulated. At 15 dpi, the accumulation of flavonoids were still significantly reduced upon CCYV infection, while lipids, amino acids, nucleotides and derivatives were remarkably enhanced. Most of significantly increased metabolites were lipids (lysophosphatidylethanolamine, LPE; lysophosphatidylcholine, LPC and their isomers). Also, the number of significantly changed metabolites increased with the infection period. However, only a few organic acids and phenolic acids showed difference between CCYV-infected and healthy cucumber plants. Conclusions CCYV infection repressed the defensive flavonoids, terpeneoids metabolism but triggered the lipids, amino acids and nucleotides metabolism with the inoculation period. This result suggests that CCYV-infection makes cucumber plants more susceptible for whiteflies attack and CCYV infection. The reduction of defensive comounds and the increase of amino acids may be partially responsible for enhancing feeding preference of whiteflies to CCYV-infected hosts. CCYV may hijacked lipid metabolism for virus replication and assembly.

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“…CCYV is regulated by a protein P4.9 [29]. As a result of systemic infection, plants usually develop interveinal yellowing symptoms typical of CCYV [30].…”

Section: Characterization Of Nanoparticlesmentioning

confidence: 99%

“…In general, the cell-to-cell movement of plant viruses depends on the movement protein (MP) that modifies the size exclusion limit of the plasmodesmata (PD) to facilitate viral genome transport to adjacent, uninfected cells [27,28]. Similarly, other research has shown that the systemic movement of CCYV is regulated by a protein P4.9 [29]. As a result of systemic infection, plants usually develop interveinal yellowing symptoms typical of CCYV [30].…”

Section: Introductionmentioning

confidence: 99%

Manufactured Nano-Objects Confer Viral Protection against Cucurbit Chlorotic Yellows Virus (CCYV) Infecting Nicotiana benthamiana

et al. 2022

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Nanotechnology has emerged as a new tool to combat phytopathogens in agricultural crops. Cucurbit chlorotic yellows virus (CCYV) mainly infects Solanaceae crops and causes significant crop losses. Nanomaterials (NMs) may have efficacy against plant viruses, but the mechanisms underlying complex nanomaterials-plant-virus interactions remain elusive. We challenged Nicotiana benthamiana plants with GFP-tagged CCYV and observed morphological, physiological, and molecular changes in response to 21-d foliar exposure to nanoscale Fe and Zn and C60 fullerenes at 100 mg/L concentration for 21 days. We observed that in response to C60 (100 mg/L) treatment, plants displayed a normal phenotype while the viral infection was not seen until 5 days post-inoculation. On the contrary, Fe and Zn were unable to suppress viral progression. The mRNA transcriptional analysis for GFP and viral coat protein revealed that the transcripts of both genes were 5-fold reduced in response to C60 treatment. Evaluation of the chloroplast ultrastructure showed that NMs treatment maintained the normal chloroplast structure in the plants as compared to untreated plants. C60 upregulated the defense-related phytohormones (abscisic acid and salicylic acid) by 42–43%. Our results demonstrate the protective function of carbon-based NMs, with suppression of CCYV symptoms via inhibition of viral replication and systemic movement.

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Identification of cucurbit chlorotic yellows virus P4.9 as a possible movement protein

Cited by 8 publications

References 20 publications

Discovery and Genomic Function of a Novel Rice Dwarf-Associated Bunya-like Virus

Discovery and Genomic Function of a Novel Rice Dwarf-Associated Bunya-like Virus

Widely targeted analysis of metabolomic changes of Cucumis sativus induced by cucurbit chlorotic yellows virus

Manufactured Nano-Objects Confer Viral Protection against Cucurbit Chlorotic Yellows Virus (CCYV) Infecting Nicotiana benthamiana

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