Identification of Cultivable Microorganisms from Root Canals with Apical Periodontitis Following Two-Visit Endodontic Treatment with Antibiotics/Steroid or Calcium Hydroxide Dressings

Chu, Frederick C.S.; Leung, W. Keung; Tsang, P.W.M.; Chow, Tak W; Samaranayake, LP

doi:10.1016/j.joen.2005.10.014

Cited by 56 publications

(51 citation statements)

References 27 publications

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“…The superior in vitro antimicrobial activity of the combination of Ca(OH) 2 with CMCP has long been recognized. Roach et al (4) reported that Ca(OH) 2 and Ca(OH) 2 associated with CMCP inhibited Enterococcus faecalis longer than the Ca(OH) 2 points and chlorhexidine.…”

Section: Discussionmentioning

confidence: 99%

“…Although chemomechanical preparation of infected root canals has been shown to be effective in reducing the root canal microbiota, viable microorganisms can be often isolated in the posttreatment period (1). Such residual microorganisms and their byproducts remaining in the apical portion of the root canal or in the dentinal tubules may be a cause of endodontic failure (2). Another important reason for the clinical failure of endodontic therapy is coronal leakage (3).…”

Section: Introductionmentioning

confidence: 99%

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Bacterial leakage in root canals filled with calcium hydroxide paste associated with different vehicles

et al. 2008

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This in vitro study evaluated, using a bacterial leakage model, whether intracanal medication with calcium hydroxide [Ca(OH) 2 ]-based pastes prepared with different vehicles, has inhibitory effect on corono-apical leakage of bacteria. Forty instrumented human canines were dressed with Ca(OH) 2 p.a. associated with: G1= distilled water; G2= polyethylene glycol (PG); G3= PG + CMCP; and G4= glycerin. Five teeth with intact crowns served as negative controls and 5 instrumented teeth without temporary dressing served as positive controls. All teeth were mounted in a 2-chamber apparatus and then exposed to human saliva for 63 days. Leakage was recorded when turbidity was observed in the lower chamber. Fifty percent of the samples of G1 and G2, 10% of G3 and 80% of G4 were fully contaminated after 9 weeks. Statistically significant differences were observed with paired comparisons G3 and G4 (p=0.0069), with G3 achieving better seal against bacterial leakage than G4.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bacterial leakage in root canals filled with calcium hydroxide paste associated with different vehicles

et al. 2008

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“…Infected root canals are colonized by a consortium of bacterial species (Chu et al, 2005(Chu et al, , 2006. 16S rDNA sequencing has shown that the infected canals contain a more diverse flora than previously recognized (Vickerman et al, 2007), but surprisingly symptomatic root canal lesions harbor a less diverse flora than do asymptomatic lesions.…”

Section: Endodontic Infectionsmentioning

confidence: 98%

Exploring the oral bacterial flora: current status and future directions

et al. 2010

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Oral Diseases (2010) 16, 136–145 Objective: The oral cavity forms an indispensable part of the human microbiome, for its unique and diverse microflora distributed within various niches. While majority of these organisms exhibit commensalism, shifts in bacterial community dynamics cause pathological changes within oral cavity and distant sites. The aim of this review was to appraise the current and emerging methods of detecting bacteria of the oral cavity paying particular attention to the cultivation independent methods. Design: Literature pertaining to cultivation based and cultivation independent methods of oral bacterial identification was reviewed. Methods: The specific advantages and disadvantages of cultivation based, microscopic, immunological and metagenomic identification methods were appraised. Results: Because of their fastidious and exacting growth requirements, cultivation based studies grossly underestimate the extent of bacterial diversity in these polymicrobial infections. Culture independent methods deemed more sensitive in identifying difficult to culture and novel bacterial species. Conclusion: Apart from characterizing potentially novel bacterial species, the nucleic acid sequence data analyzed using various bioinformatics protocols have revealed that there are in excess of 700 bacterial species inhabiting the mouth. Moreover, the latest pyrosequencing based methods have further broadened the extent of bacterial diversity in oral niches.

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“…In addition to limited antimicrobial activity, tetracyclines another commercial product for intracanal use ) [17]. It contains two antibiotics, neomycin and polymyxin B sulphate, but the effect against endodontic flora is not better than with calcium hydroxide (Tang et al 2004, Chu et al 2006 [18,19]. The use of topical antibiotics in root canal treatment has also been proposed to prevent or reduce postoperative symptoms.…”

Section: A Antibiotics During Root Canal Treatmentmentioning

confidence: 99%

“…The paradigm of this model of treatment is the prevention of bacterial endocarditis, indicated in risk patients in the context of any invasive procedure within the oral cavity and following the guidelines of the American Health Association (AHA) [22].…”

Section: Iiib Prophylaxis Of Focal Infectionmentioning

confidence: 99%