Identification of Culturable and Nonculturable Microorganisms, Lipopolysaccharides, and Lipoteichoic Acids From Root Canals of Teeth With Endodontic Failure

Gomes, Brenda P. F. A.; Francisco, Priscila Amanda; Godoi, Ederaldo Pietrafesa; Endo, Marcos Sérgio; Barbosa-Ribeiro, Marlos; Delboni, Maraisa Greggio; Pecorari, Vanessa Gallego Arias

doi:10.1016/j.joen.2021.04.011

Cited by 23 publications

(19 citation statements)

References 56 publications

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“…Pereira et al (2017) used real-time PCR to test ten important microorganisms collected from the root apex and the surrounding apical lesion. Fusobacterium nucleatum was the most prevalent and significant species in the root canal and periradicular lesions (Pereira et al, 2017;Gomes et al, 2021). Meanwhile, Noguchi et al (2005) found that F. nucleatum was frequently detected in extraradicular biofilm attached to the surface of root ends with persistent infection.…”

Section: Discussionmentioning

confidence: 99%

“…(2017) used real-time PCR to test ten important microorganisms collected from the root apex and the surrounding apical lesion. Fusobacterium nucleatum was the most prevalent and significant species in the root canal and periradicular lesions ( Pereira et al., 2017 ; Gomes et al., 2021 ). Meanwhile, Noguchi et al.…”

Section: Discussionmentioning

confidence: 99%

“…In the present study, Porphyromonas , Eubacterium , Treponema , Phocaeicola , Tannerella , and Prevotella were more associated with infections with the sinus tract. Porphyromonas , a genus of small anaerobic gram-negative nonmotile cocci, play an essential role in endodontic infections in light of its prominent virulence factors ( Barbosa-Ribeiro et al., 2021 ; Bordagaray et al., 2021 ; Gomes et al., 2021 ) The sinus tract may be related to the simultaneous occurrences of both Porphyromonas endodontalis and Tannerella forsythia , P. gingivalis , and P. endodontalis , or Parvimonas micra and P. endodontalis ( Qi et al., 2016 ). Bacterial communities of primary intraradicular infections with or without sinus tracts were compared, and a higher abundance of P. gingivalis and F. nucleatum sp.…”

Section: Discussionmentioning

confidence: 99%

“…The samples without sinus tracts were characterized by Microbacterium and Enterococcus . Enterococcus , a genus of gram-positive, facultatively anaerobic bacteria, was much more likely to be found in cases of persistent infections than in primary infections due to its ability to invade the dentinal tubules and highly resistant to antimicrobial strategies ( Sun et al., 2009 ; Murad et al., 2014 ; Keskin et al., 2017 ; Bouillaguet et al., 2018 ; Sun et al., 2018 ; Barbosa-Ribeiro et al., 2021 ; Gomes et al., 2021 ; Zhang et al., 2021 ). In the present study, Enterococcus was consistently detected in all of the samples but with a low mean proportion of OTUs.…”

Section: Discussionmentioning

confidence: 99%

“…By using transmission electron microscopy, complex multispecies biofilm was present in root canals of persistent apical periodontitis (Carr et al, 2009). Fusobacterium, Corynebacterium, Porphyromonas, Streptococcus, and Stenotrophomonas were the most abundant genera detected in intraradicular samples of persistent infection (Henriques et al, 2016;Takahama et al, 2018;Gomes et al, 2021;Hou et al, 2021). Moreover, Pseudomonas spp., Burkholderia spp., and Enterococcus faecalis were also found highly prevalent in root canals with persistent endodontic infections (Siqueira et al, 2011;Barbosa-Ribeiro et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Microbial Communities in the Extraradicular and Intraradicular Infections Associated With Persistent Apical Periodontitis

Sun

Yang

Nie

et al. 2022

Front. Cell. Infect. Microbiol.

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Microorganisms in the complex root canal system and the extraradicular regions, including the periapical lesions and extraradicular biofilm may cause root canal treatment failures. However, few studies described the difference between the intraradicular and extraradicular infections from the same tooth associated with persistent apical periodontitis. This study aimed to characterize the microbiome present in the root canal, extraradicular biofilm, and periapical lesions associated with persistent apical periodontitis. The microbial communities in the root canal, extraradicular biofilm, and periapical lesions were investigated by Illumina high-throughput sequencing using Illumina Hiseq 2500 platform. The dominant phyla in the extraradicular and intraradicular infections associated with persistent apical periodontitis were Proteobacteria, Firmicutes, Bacteroidetes, and Actinobacteria, and the genera Fusobacterium, Morganella, Porphyromonas, Streptococcus, and Bifidobacterium dominated across all samples. Although extraradicular infection sites showed higher OTU richness and β-diversity compared to intraradicular samples, the occurrence of sinus tract rather than the sampling sites demarcated the microbial communities in the infections associated with persistent apical periodontitis. PERMANOVA analysis confirmed that the samples with or without sinus tracts contained significantly different microbial communities. Porphyromonas, Eubacterium, Treponema, and Phocaeicola were found in significantly higher levels with sinus tracts, whilst Microbacterium and Enterococcus were more abundant in samples without sinus tracts. In conclusion, diverse bacteria were detected in both intraradicular and extraradicular infections associated with persistent apical periodontitis, which might be influenced by the occurrence of the sinus tract. The results may provide new insight into the pathogenesis of persistent apical periodontitis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Microbial Communities in the Extraradicular and Intraradicular Infections Associated With Persistent Apical Periodontitis

Sun

Yang

Nie

et al. 2022

Front. Cell. Infect. Microbiol.

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Coaggregated E. faecalis with F. nucleatum regulated environmental stress responses and inflammatory effects

Zhou,

Yuan,

Yang

et al. 2024

Appl Microbiol Biotechnol

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To investigate the cell-cell interactions of intergeneric bacterial species, the study detected the survival of Enterococcus faecalis (Ef) under monospecies or coaggregation state with Fusobacterium nucleatum subsp. polymorphum (Fnp) in environmental stress. Ef and Fnp infected the human macrophages with different forms (Ef and Fnp monospecies, Ef-Fnp coaggregates, Ef + Fnp cocultures) for exploring the immunoregulatory effects and the relevant molecular mechanisms. Meanwhile, the transcriptomic profiles of coaggregated Ef and Fnp were analyzed. Ef was shown to coaggregate with Fnp strongly in CAB within 90 min by forming multiplexes clumps. Coaggregation with Fnp reinforced Ef resistance against unfavorable conditions including alkaline, hypertonic, nutrient-starvation, and antibiotic challenges. Compared with monospecies and coculture species, the coaggregation of Ef and Fnp significantly facilitates both species to invade dTHP-1 cells and aid Ef to survive within the cells. Compared with coculture species, dual-species interaction of Ef and Fnp significantly decreased the levels of pro-inflammatory cytokines IL-6, TNF-α, and chemokines MCP-1 secreted by dTHP-1 cells and lessened the phosphorylation of p38, JNK, and p65 signaling pathways. The transcriptome sequencing results showed that 111 genes were differentially expressed or Ef-Fnp coaggregated species compared to Ef monospecies; 651 genes were differentially expressed for Fnp when coaggregation with Ef. The analysis of KEGG pathway showed that Ef differentially expressed genes (DEGs) were enriched in quorum sensing and arginine biosynthesis pathway; Fnp DEGs were differentially concentrated in lipopolysaccharide (LPS) biosynthesis, biofilm formation, and lysine degradation pathway compared to monospecies. Key points • Coaggregated with Fnp aids Ef’s survival in environmental stress, especially in root canals after endodontic treatment. • The coaggregation of Ef and Fnp may weaken the pro-inflammatory response and facilitate Ef to evade killed by macrophages. • The coaggregation between Ef and Fnp altered interspecies transcriptional profiles.

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Microbiological profile of root canals indicated for endodontic retreatment due to secondary endodontic infections or for prosthetic reasons

et al. 2023

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Identification of Culturable and Nonculturable Microorganisms, Lipopolysaccharides, and Lipoteichoic Acids From Root Canals of Teeth With Endodontic Failure

Cited by 23 publications

References 56 publications

Microbial Communities in the Extraradicular and Intraradicular Infections Associated With Persistent Apical Periodontitis

Microbial Communities in the Extraradicular and Intraradicular Infections Associated With Persistent Apical Periodontitis

Coaggregated E. faecalis with F. nucleatum regulated environmental stress responses and inflammatory effects

Microbiological profile of root canals indicated for endodontic retreatment due to secondary endodontic infections or for prosthetic reasons

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