Identification of Cytotoxic T lymphocyte (CTL) Epitope and design of an immunogenic multi-epitope of Bovine Ephemeral Fever Virus (BEFV) Glycoprotein G for Vaccine Development

Molazadeh, Shima; Bakhshesh, Mehran; Keivanfar, Hadi; Brujeni, Gholamreza Nikbakht

doi:10.1016/j.rvsc.2021.12.023

Cited by 6 publications

(2 citation statements)

References 33 publications

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“…However, ORFV's zoonotic nature and robust immune response in both humans and ruminants and its interaction with HLA may suggest the possibility of putative epitopes ( Rohde et al., 2012 ; Bergqvist et al., 2017 ; Demiraslan et al., 2017 ; Wang et al., 2019 ). We referred to studies on zoonosis and other animal diseases that used known MHC phenotypes for epitope screening ( Palanisamy and Lennerstrand, 2017 ; Joshi et al., 2021 ; Mollazadeh et al., 2022 ; Soltan et al., 2022 ). These candidate epitopes could be considered for vaccine development involving host cell immunity.…”

Section: Discussionmentioning

confidence: 99%

Genetic Analysis of Orf Virus (ORFV) Strains Isolated from Goats in China: Insights into Epidemiological Characteristics and Evolutionary Patterns

Tian

Zhu

et al. 2023

Virus Research

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Section: Discussionmentioning

confidence: 99%

Genetic Analysis of Orf Virus (ORFV) Strains Isolated from Goats in China: Insights into Epidemiological Characteristics and Evolutionary Patterns

Tian

Zhu

et al. 2023

Virus Research

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“…Since no HTL epitopes were assigned due to the lack of a complete characterization of bovine MHC II molecules, only CTL and B cell epitopes were used to construct a multi-epitope vaccine (Mollazadeh et al 2022 ). Immunogenic, non-allergenic, antigenic, and non-toxic epitopes were selected among these epitopes because they should elicit an immune response without endangering the host.…”

Section: Resultsmentioning

confidence: 99%

In silico analysis of virulence factors of Streptococcus uberis for a chimeric vaccine design

Yılmaz Çolak

2024

In Silico Pharmacol.

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Streptococcus uberis is one of the causative agents of bovine mastitis, which has detrimental effects on animal health and the dairy industry. Despite decades of research, the requirement for effective vaccines against the disease remains unmet. The goal of this study was to create a multi-epitope vaccine using five virulence factors of S. uberis through the reverse vaccinology approach, which has been employed due to its high efficiency and applicability. Plasminogen activator A (PauA), glyceraldehyde-3-phosphate dehydrogenase C (GapC), C5a peptidase, S. uberis adhesion molecule (SUAM), and sortase A (SrtA) were selected for the T cytotoxic (CTL) and B cell epitope analyses as they were extensively studied in S. uberis or other pathogens. Eighteen CTL and ten B cell epitopes that were antigenic, non-toxic, and non-allergenic were selected in order to design a chimeric vaccine candidate that in silico analysis revealed to be potentially immunogenic, non-allergenic, and stable. Molecular docking analysis of the vaccine candidate with Toll-like receptor (TLR) 2 and TLR 4 revealed stable interactions between the candidate and the immune receptors. Meanwhile, the stability of the docked complexes was confirmed using normal mode analysis. Additionally, in silico immune simulation of the vaccine candidate demonstrated the stimulation of primary immune responses, indicating that the chimeric protein can hold promise as a viable vaccine candidate for preventing S. uberis mastitis. Moreover, the current study can provide a background for designing epitope-based vaccines based on the explored epitopes.

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Hydrogels based on schizophyllan

Hou,

2024

Polysaccharide Hydrogels for Drug Delivery and Regenerative Medicine

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Identification of Cytotoxic T lymphocyte (CTL) Epitope and design of an immunogenic multi-epitope of Bovine Ephemeral Fever Virus (BEFV) Glycoprotein G for Vaccine Development

Cited by 6 publications

References 33 publications

Genetic Analysis of Orf Virus (ORFV) Strains Isolated from Goats in China: Insights into Epidemiological Characteristics and Evolutionary Patterns

Genetic Analysis of Orf Virus (ORFV) Strains Isolated from Goats in China: Insights into Epidemiological Characteristics and Evolutionary Patterns

In silico analysis of virulence factors of Streptococcus uberis for a chimeric vaccine design

Hydrogels based on schizophyllan

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