Identification of DAPK as a scaffold protein for the LIMK/cofilin complex in TNF-induced apoptosis

Biofabrication of 3D Alginate-Based Hydrogel for Cancer Research: Comparison of Cell Spreading, Viability, and Adhesion Characteristics of Colorectal HCT116 Tumor Cells

“…However, the disadvantage of 2D monolayer culture models is the lack of 3D microenvironment of intact tissue (3). This is of crucial importance as cell-cell, cell-matrix interactions and interstitial fluid influence differentiation and cellular functions within the 3D network (2). Therefore, in vitro 3D tumor models with human cancer cells have attracted scientists to precisely imitate the features of human cancer tissues (4)(5)(6).…”

“…To mimic the structural architecture and cellular functions of the extracellular matrix (ECM) in 3D model systems some major characteristics have to be considered: suitable mechanical characteristics and chemical composition, facilitation of cell growth and maintenance, and fostering the transport of nutrients, gas, and metabolic waste, as well as signal transduction (7,9,10). The main advantage of 3D cultures in comparison to 2D monolayers is their clearly defined geometry that can help to co-culture different cell types with diverse phenotypes that are often found in solid epithelial tumors (2). Furthermore, the interaction between epithelial or tumor cells with stromal cells is possible in 3D systems, and the more in vivo-like environment seems to be a better test system for evaluating drug treatment responses (2).…”

“…The main advantage of 3D cultures in comparison to 2D monolayers is their clearly defined geometry that can help to co-culture different cell types with diverse phenotypes that are often found in solid epithelial tumors (2). Furthermore, the interaction between epithelial or tumor cells with stromal cells is possible in 3D systems, and the more in vivo-like environment seems to be a better test system for evaluating drug treatment responses (2). Therefore, it is challenging to introduce 3D culture systems into This article has been peer-reviewed and accepted for publication, but has yet to undergo copyediting and proof correction.…”

“…Colorectal cancer is symptomatic only if progressing to advanced stages, thus the implementation of new model systems for understanding the tumorigenesis of this cancer type should help to design new cancer therapy protocols. Previously, 2 dimensional (2D) monolayers and organ cultures were the in vitro models of choice for cancer research (2).…”

Chronophin Dimerization Is Required for Proper Positioning of Its Substrate Specificity Loop

“…Chronophin is a homodimeric, C2a-capped HAD phosphatase (9 -11) known to target Ser 3 -phosphocofilin (12)(13)(14)(15)(16) and the Ser/Thr-phosphorylated steroid receptor co-activators 1 and 3 (17). In addition to its emerging protein phosphatase activities, chronophin (gene name, pyridoxal phosphatase (PDXP)) acts as a small molecule phosphatase dedicated to the metabolism of pyridoxal 5Ј-phosphate (PLP) (10,11).…”