Identification of dauricine and its metabolites in rat urine by liquid chromatography–tandem mass spectrometry

Han, Fengmei; Peng, Zhihong; Song, Wei; Zhang, H.M.; Zhu, Mingming; Chen, Y.

doi:10.1016/j.jchromb.2007.03.036

Cited by 23 publications

(17 citation statements)

References 14 publications

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“…administration. Our results supported the notion that LC-MS/MS method was sensitive, simple, and accurate to measure dauricine [13,14]. In addition, from the current study, we obtained the complete pharmacokinetic data of dauricine following administration via three different routes.…”

Section: Discussionsupporting

confidence: 85%

“…and i.p., we chose rat as a model for the current study. Consistent with previous studies [13,14], our results indicated that LC-MS/MS method is sensitive, simple and accurate. To our best knowledge, we, for the first time, present the pharmacokinetic data of dauricine following administration in three different routes (i.v., p.o., and i.p.…”

Section: Introductionsupporting

confidence: 92%

“…Chromatographic methods for dauricine detection have been previously reported [13,14], however, the bioavailability of dauricine after administration in different routes has not been investigated. Therefore, the primary goal of this study was to investigate dauricine bioavailability following p.o.…”

Section: Discussionmentioning

confidence: 99%

“…For example, using LC-MS/MS, Han et al analyzed dauricine in rat urine [13]; and Liu et al examined dauricine in human plasma [14]. However, those studies characterized dauricine only after oral administration.…”

Section: Introductionmentioning

confidence: 99%

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Using LC-MS/MS to study dauricine pharmacokinetics and determine its bioavailability following administration in different routes

Gao¹,

Jiang²,

Wang³

2013

Acta Chromatographica

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Summary.Dauricine has a variety of pharmacological properties including anti-inflammatory, anti-arrhythmic, and antihypertensive effects as well as reversing multidrug resistance (MDR) of cancer cells. While its therapeutic application is increasing, its bioavailability of different administration routes has not been studied. In the present study, we developed and validated a liquid chromatography/electrospray ionization mass spectrometry method (LC-MS/MS). Using this method, we quantified dauricine in rat plasma after administration via intravenous (i.v.) injection, per oral (p.o.), and intraperitoneal injection (i.p.). Our results indicated that this method detected plasma dauricine with a good linearity in the range of 1.95-1000.00 ng/mL (r = 0.9997). The extraction method showed an average intra-and inter-day recovery of 98.21-104.35% and 98.0-103.58%, respectively. Dauricine showed a fast absorption and widespread distribution after administration in all three tested routes. After intravenous administration (2.5, 5.0, 10.0 mg/kg), the pharmacokinetics of dauricine exhibited a first-order kinetics. In addition, dauricine showed a slow elimination with a long half-life (t 1/2z ) and double peaks phenomenon following p.o. and i.p. administration. Furthermore, using area under the plasma concentration-time curve (AUC), we calculated absolute bioavailability, which was over twofold higher when administered via i.p. than via p.o. administration. The newly obtained information from our study will provide important reference for dauricine dose and administration route in designing dauricine therapy for applicable diseases.

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Section: Discussionsupporting

confidence: 85%

Section: Introductionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Using LC-MS/MS to study dauricine pharmacokinetics and determine its bioavailability following administration in different routes

Gao¹,

Jiang²,

Wang³

2013

Acta Chromatographica

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“…HPLC-MS n is a powerful modern tool for the identification of substances in biofluids (Ge et al, 2007;Han et al, 2007;Lam and Ramanathan, 2002;Rudewicz and Straub, 1986;Wang et al, 2005;Yang et al, 2006;Yu et al, 1999;Zhang et al, 2003Zhang et al, , 2004. This approach has the advantages of sensitivity and specificity, and is considered less time-consuming and labor-intensive than other methods, such as HPLC and GC-MS.…”

Section: Introductionmentioning

confidence: 99%

Identification of major alkaloids and steroidal saponins in rat serum by HPLC‐diode array detection‐MS/MS following oral administration of Huangbai‐Zhimu herb‐pair Extract

Fan

Tang

et al. 2008

Biomedical Chromatography

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Huangbai-Zhimu herb-pair (HBZMHP) is a widely used Chinese traditional medicine formula in treating various diseases; however, its active components have remained unknown. In this paper, serum chemistry and combined high-performance liquid chromatography (HPLC), diode-array detection and mass-spectrometry (MS) techniques were used to study the constituents of HBZMHP extract absorbed into rat serum after oral administration. A total of nine characteristic HPLC peaks in the TIC chromatograms were identified as magnoflorine (1), menisperine (2), palmatine (3), berberine (4), timosaponin N or timosaponin E1 (5), timosaponin D (6), timosaponin BIII, anemarsaponin C or xilingsaponin B (7) timosaponin BII (8) and timosaponin AIII (9). All of the identified peaks were constituents of HBZMHP extract. The results narrow the range of active compounds to be found in HBZMHP extract, and pave the way for the follow-up action mechanism research.

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Profiling and Characterization of Bioactive Substances of Herbal Medicine and their Metabolites Using Mass Spectrometry

Chen

2014

Handbook of Metabolic Pathways of Xenobiotics

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Identification of dauricine and its metabolites in rat urine by liquid chromatography–tandem mass spectrometry

Cited by 23 publications

References 14 publications

Using LC-MS/MS to study dauricine pharmacokinetics and determine its bioavailability following administration in different routes

Using LC-MS/MS to study dauricine pharmacokinetics and determine its bioavailability following administration in different routes

Identification of major alkaloids and steroidal saponins in rat serum by HPLC‐diode array detection‐MS/MS following oral administration of Huangbai‐Zhimu herb‐pair Extract

Profiling and Characterization of Bioactive Substances of Herbal Medicine and their Metabolites Using Mass Spectrometry

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